Type III collagen is essential for growth acceleration of human osteoblastic cells by ascorbic acid 2-phosphate, a long-acting vitamin C derivative

Role of Hepatocyte Growth Factor in Promoting the Growth of Human Corneal Endothelial Cells Stimulated byl-Ascorbic Acid 2-Phosphate

“…We examined extracellular matrix from HCEC sheets by x-z projection imaging and confirmed that Asc-2P did not promote accumulation of these collagens (Kimoto M, unpublished observation, 2010). These findings also differ from previous reports on various mesenchymal cells, including fibroblasts, 13 osteoblasts, 12,15 keratocytes, 35 and mesenchymal stem cells, 16 which showed that Asc-2P dramatically increases collagen production. Although we cannot explain this discrepancy, the following possibilities can be considered.…”

“…[12][13] We previously reported that Asc-2P significantly increased the growth and replicative lifespan of cultured HCEC. 14 The mechanisms by which Asc-2P stimulates cell growth are not well understood, but upregulation of collagen synthesis may be involved, because Asc plays a role as a cofactor in collagen synthesis 13 and it promotes both growth and collagen synthesis by various mesenchymal cells, such as fibroblasts, 13 osteoblasts, 12,15 and mesenchymal stem cells. 16 Another possible mechanism of growth stimulation by Asc-2P is its antioxidant activity.…”

Vitamin C in Disease Prevention and Cure: An Overview

“…In an experimental study AA has been shown to have involvement in synthesis and release of type IV collagen into the culture medium [21]. Further, it has also been reported that AA 2-phosphate, a long-acting vitamin C derivative, stimulates both cell growth and the expression of mRNA for type III collagen in human osteoblast-like MG-63 cells and in normal human osteoblasts, as well as in human bone marrow mesenchymal stem cells, but not the expression of type I collagen in these cells [22]. However, in another study Kishimoto et al [23] have observed that AA induced the expression of type 1 and type 4 collagen and SVCT2 in cultured human skin fibroblasts.…”

Wound healing and antioxidant properties: do they coexist in plants?

“…These deficiency impair the proline and lysine hydroxylation which result in a synthesis of low resistance collagen and consequently in cicatrization deficiency. [12] Research into the role of antioxidants from plants in wound-healing has been widely published. [10,13,14] This review aims to appraise published literature and evaluate whether wound-healing and antioxidant properties coexist in plants.…”