Type II myosin gene in Fusarium graminearum is required for septation, development, mycotoxin biosynthesis and pathogenicity

Song, Bo; Li, Heping; Zhang, Jingbo; Wang, Jianhua; Gong, An-Dong; Song, Xiu-Shi; Chen, Thomas; Liao, Yu-Cai

doi:10.1016/j.fgb.2013.02.010

Cited by 23 publications

(23 citation statements)

References 50 publications

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“…The consequences include failure to form septa and failure to conidiate. Both of these effects are consistent with earlier experimental work with myosin II deletion mutants in filamentous fungi (Cánovas et al, 2011;Song et al, 2013;Taheri-Talesh et al, 2012). Although the exact function of the CAR in septation has not yet been clearly established, its capacity to constrict in conjunction with membrane ingrowth and crosswall synthesis is an absolute requirement for septum formation in vegetative hyphae and in conidiation.…”

Section: Discussionsupporting

confidence: 89%

“…Despite this lack of diversity, fungal myosins play essential roles in a wide range of functions, ranging from cytokinesis to endocytosis and vesicle motility (Steinberg, 2000). Deletion of myosin II genes in A. nidulans (Taheri-Talesh et al, 2012), Fusarium solani (Song et al, 2013), and Penicillium marneffei (Cánovas et al, 2011) effectively blocks both septation and conidiation. Type II myosin, like actin, goes through a stepwise process of coalescence at septation sites (Delgado-Álvarez et al, 2014;Taheri-Talesh et al, 2012), appearing first as cytoplasmic strings before condensing into a compact cortical ring.…”

Section: Introductionmentioning

confidence: 99%

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A mutation in the converter subdomain of Aspergillus nidulans MyoB blocks constriction of the actomyosin ring in cytokinesis

Hill¹,

Jackson‐Hayes²,

Wang³

et al. 2015

Fungal Genetics and Biology

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

A mutation in the converter subdomain of Aspergillus nidulans MyoB blocks constriction of the actomyosin ring in cytokinesis

Hill¹,

Jackson‐Hayes²,

Wang³

et al. 2015

Fungal Genetics and Biology

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“…myoB is required for normal septation, as deletion of myoB resulted in significant lateral or central joining defects. MyoB also localized transiently to the septa and in rings in the hyphae at presumed sites of septation, consistent with MyoB's known role as part of the actin-myosin ring to form septa (5,21,(36)(37)(38). In contrast to the ⌬myoB strain in A. nidulans, A. fumigatus myoB is dispensable for radial growth, thus displaying diverse functional contributions within the same genus (5).…”

Section: Discussionmentioning

confidence: 85%

Distinct Roles of Myosins in Aspergillus fumigatus Hyphal Growth and Pathogenesis

et al. 2016

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cMyosins are a family of actin-based motor proteins found in many organisms and are categorized into classes based on their structures. Class II and V myosins are known to be important for critical cellular processes, including cytokinesis, endocytosis, exocytosis, and organelle trafficking, in the model fungi Saccharomyces cerevisiae and Aspergillus nidulans. However, the roles of myosins in the growth and virulence of the pathogen Aspergillus fumigatus are unknown. We constructed single-and doubledeletion strains of the class II and class V myosins in A. fumigatus and found that while the class II myosin (myoB) is dispensable for growth, the class V myosin (myoE) is required for proper hyphal extension; deletion of myoE resulted in hyperbranching and loss of hyphal polarity. Both myoB and myoE are necessary for proper septation, conidiation, and conidial germination, but only myoB is required for conidial viability. Infection with the ⌬myoE strain in the invertebrate Galleria mellonella model and also in a persistently immunosuppressed murine model of invasive aspergillosis resulted in hypovirulence, while analysis of bronchoalveolar lavage fluid revealed that tumor necrosis factor alpha (TNF-␣) release and cellular infiltration were similar compared to those of the wild-type strain. The ⌬myoE strain showed fungal growth in the murine lung, while the ⌬myoB strain exhibited little fungal burden, most likely due to the reduced conidial viability. These results show, for the first time, the important role these cytoskeletal components play in the growth of and disease caused by a known pathogen, prompting future studies to understand their regulation and potential targeting for novel antifungal therapies.

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“…Actin plays a crucial role in cytokinesis in fungi (Berepiki et al ., ) and the Fgcdc14 mutant was defective in the formation of an actin ring during septation, which may be directly related to its defects in septation. Interestingly, the Fgcdc14 mutant phenotypes are similar to those of associated with loss of type II myosin Myo2 (Song et al ., ), a molecular motor that generates force for cytokinesis by interacting with actin filaments in the contractile ring (CR). In S. cerevisiae , some myo1 mutant cells appeared to form chains or were aberrantly large and/or multinucleate (Bi et al ., ).…”

Section: Discussionmentioning

confidence: 99%

FgCDC14 regulates cytokinesis, morphogenesis, and pathogenesis in Fusarium graminearum

Melesse

Zhang

et al. 2015

Molecular Microbiology

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SummaryMembers of Cdc14 phosphatases are common in animals and fungi, but absent in plants. Although its orthologs are conserved in plant pathogenic fungi, their functions during infection are not clear. In this study, we showed that the CDC14 ortholog is important for pathogenesis and morphogenesis in Fusarium graminearum. FgCDC14 is required for normal cell division and septum formation and FgCdc14 possesses phosphatase activity with specificity for a subset of Cdk-type phosphorylation sites. The Fgcdc14 mutant was reduced in growth, conidiation, and ascospore formation. It was defective in ascosporogenesis and pathogenesis. Septation in Fgcdc14 was reduced and hyphal compartments contained multiple nuclei, indicating defects in the coordination between nuclear division and cytokinesis. Interestingly, foot cells of mutant conidia often differentiated into conidiogenous cells, resulting in the production of inter-connected conidia. In the interphase, FgCdc14-GFP localized to the nucleus and spindlepole-body. Taken together, our results indicate that Cdc14 phosphatase functions in cell division and septum formation in F. graminearum, likely by counteracting Cdk phosphorylation, and is required for plant infection.

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Type II myosin gene in Fusarium graminearum is required for septation, development, mycotoxin biosynthesis and pathogenicity

Cited by 23 publications

References 50 publications

A mutation in the converter subdomain of Aspergillus nidulans MyoB blocks constriction of the actomyosin ring in cytokinesis

A mutation in the converter subdomain of Aspergillus nidulans MyoB blocks constriction of the actomyosin ring in cytokinesis

Distinct Roles of Myosins in Aspergillus fumigatus Hyphal Growth and Pathogenesis

FgCDC14 regulates cytokinesis, morphogenesis, and pathogenesis in Fusarium graminearum

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