A mutation in the converter subdomain of Aspergillus nidulans MyoB blocks constriction of the actomyosin ring in cytokinesis

Hill, Terry W.; Jackson‐Hayes, Loretta; Wang, Xiao; Hoge, Brianna L.

doi:10.1016/j.fgb.2015.01.010

Cited by 6 publications

(2 citation statements)

References 59 publications

(66 reference statements)

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“…myoB is required for normal septation, as deletion of myoB resulted in significant lateral or central joining defects. MyoB also localized transiently to the septa and in rings in the hyphae at presumed sites of septation, consistent with MyoB's known role as part of the actin-myosin ring to form septa (5,21,(36)(37)(38). In contrast to the ⌬myoB strain in A. nidulans, A. fumigatus myoB is dispensable for radial growth, thus displaying diverse functional contributions within the same genus (5).…”

Section: Discussionmentioning

confidence: 86%

Distinct Roles of Myosins in Aspergillus fumigatus Hyphal Growth and Pathogenesis

et al. 2016

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cMyosins are a family of actin-based motor proteins found in many organisms and are categorized into classes based on their structures. Class II and V myosins are known to be important for critical cellular processes, including cytokinesis, endocytosis, exocytosis, and organelle trafficking, in the model fungi Saccharomyces cerevisiae and Aspergillus nidulans. However, the roles of myosins in the growth and virulence of the pathogen Aspergillus fumigatus are unknown. We constructed single-and doubledeletion strains of the class II and class V myosins in A. fumigatus and found that while the class II myosin (myoB) is dispensable for growth, the class V myosin (myoE) is required for proper hyphal extension; deletion of myoE resulted in hyperbranching and loss of hyphal polarity. Both myoB and myoE are necessary for proper septation, conidiation, and conidial germination, but only myoB is required for conidial viability. Infection with the ⌬myoE strain in the invertebrate Galleria mellonella model and also in a persistently immunosuppressed murine model of invasive aspergillosis resulted in hypovirulence, while analysis of bronchoalveolar lavage fluid revealed that tumor necrosis factor alpha (TNF-␣) release and cellular infiltration were similar compared to those of the wild-type strain. The ⌬myoE strain showed fungal growth in the murine lung, while the ⌬myoB strain exhibited little fungal burden, most likely due to the reduced conidial viability. These results show, for the first time, the important role these cytoskeletal components play in the growth of and disease caused by a known pathogen, prompting future studies to understand their regulation and potential targeting for novel antifungal therapies.

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Section: Discussionmentioning

confidence: 86%

Distinct Roles of Myosins in Aspergillus fumigatus Hyphal Growth and Pathogenesis

et al. 2016

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“…Lifeact is a 17 amino acid peptide derived from the actin-binding protein Abp140 of the budding yeast Saccharomyces cerevisiae (Riedl et al, 2008). It has previously been used in the Aspergilli as a marker for actin, but the F-actin dynamics it revealed have not been fully characterized (Katayama et al, 2012; Hill et al, in press). Here, Lifeact labeling of actin patches, cables, and rings, along with live cell imaging techniques adapted for filamentous fungi, were used to characterize actin dynamics in growing A. nidulans cells as well as during the developmental events of conidial and hyphal anastomosis (cell fusion).…”

Section: Introductionmentioning

confidence: 99%

Live Cell Imaging of Actin Dynamics in the Filamentous FungusAspergillus nidulans

et al. 2016

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Hyphal cells of filamentous fungi grow at their tips in a method analogous to pollen tube and root hair elongation. This process, generally referred to as tip growth, requires precise regulation of the actin cytoskeleton, and characterizing the various actin structures in these cell types is currently an active area of research. Here, the actin marker Lifeact was used to document actin dynamics in the filamentous fungus Aspergillus nidulans. Contractile double rings were observed at septa, and annular clusters of puncta were seen subtending growing hyphal tips, corresponding to the well-characterized subapical endocytic collar. However, Lifeact also revealed two additional structures. One, an apical array, was dynamic on the face opposite the tip, while a subapical web was dynamic on the apical face and was located several microns behind the growth site. Each was observed turning into the other over time, implying that they could represent different localizations of the same structure, although hyphae with a subapical web grew faster than those exhibiting an apical array. The subapical web has not been documented in any filamentous fungus to date, and is separate from the networks of F-actin seen in other tip-growing organisms surrounding septa or stationary along the plasmalemma.

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Septum Formation and Cytokinesis in Ascomycete Fungi

Seiler

Heilig

2019

Biology of the Fungal Cell

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A mutation in the converter subdomain of Aspergillus nidulans MyoB blocks constriction of the actomyosin ring in cytokinesis

Cited by 6 publications

References 59 publications

Distinct Roles of Myosins in Aspergillus fumigatus Hyphal Growth and Pathogenesis

Distinct Roles of Myosins in Aspergillus fumigatus Hyphal Growth and Pathogenesis

Live Cell Imaging of Actin Dynamics in the Filamentous FungusAspergillus nidulans

Septum Formation and Cytokinesis in Ascomycete Fungi

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