2014
DOI: 10.1074/jbc.m114.584185
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Type II Fatty Acid Synthesis Is Essential for the Replication of Chlamydia trachomatis

Abstract: Background: Chlamydia trachomatis has a phospholipid composition that resembles its eukaryotic host, but it contains branched-chain fatty acids of chlamydial origin. Results: The inhibition of the enoyl-acyl carrier protein reductase (FabI) in chlamydial fatty acid synthesis blocks C. trachomatis replication. Conclusion: Bacterial FASII is required for C. trachomatis proliferation. Significance: FabI is a therapeutic target against C. trachomatis.

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Cited by 43 publications
(69 citation statements)
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“…A NdeI restriction site was engineered at the 5Ј-end of the gene with the start codon in the NdeI site, whereas a His 6 tag, stop codon, and an EcoRI restriction site were sequentially engineered at the 3Ј-end of the gene. The genes were cloned into the pPJ131 plasmid (a modified version of the pBluescript plasmid with the multiple cloning site from pET28a) and the pET21a plasmid via the NdeI and EcoRI (New England Biolabs) restriction sites (24).…”
Section: Methodsmentioning
confidence: 99%
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“…A NdeI restriction site was engineered at the 5Ј-end of the gene with the start codon in the NdeI site, whereas a His 6 tag, stop codon, and an EcoRI restriction site were sequentially engineered at the 3Ј-end of the gene. The genes were cloned into the pPJ131 plasmid (a modified version of the pBluescript plasmid with the multiple cloning site from pET28a) and the pET21a plasmid via the NdeI and EcoRI (New England Biolabs) restriction sites (24).…”
Section: Methodsmentioning
confidence: 99%
“…Cells were pelleted and washed twice with 20 mM Tris, pH 8.2, and finally resuspended in 20 mM Tris, pH 8.2 (20 ml/liter of culture). Cells were lysed via a cell disruptor and the C-terminal His 6 -tagged NgFabI was purified via standard nickel chelation chromatography (24).…”
Section: Methodsmentioning
confidence: 99%
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“…Therefore, viewing AasC as an opportunistic pathway with a role in increasing the overall cellular yield of C. trachomatis by sparing energy for other biosynthetic pathways is consistent with the bacterial paradigm. Endogenous C. trachomatis fatty acid synthesis is essential for the replication of C. trachomatis on the basis of the blockade of growth by a FASII inhibitor (9). However, this fact alone cannot be used to conclude that C. trachomatis does not support its growth with host fatty acids.…”
Section: Discussionmentioning
confidence: 99%
“…Autonomous phospholipid synthesis is shown. The fatty acid synthesis module (FASII) supplies acyl-ACP to the PlsE and PlsC acyltransferases of phospholipid biosynthesis and to the lipid A acyltransferases that initiate lipo-oligosaccharide (LOS) biosynthesis, as described previously (6,9). The new enzymes characterized in this work are shown in light blue.…”
mentioning
confidence: 95%