Type I collagen promotes the migration and myogenic differentiation of C2C12 myoblasts<i>via</i>the release of interleukin-6 mediated by FAK/NF-κB p65 activation

Liu, Xiaoling; Gao, Yanfang; Long, Xinyu; Hayashi, Toshihiko; Mizuno, Kazunori; Fujisaki, Hitomi; Ogura, Takayuki; Wang, Dan Ohtan; Ikejima, Takashi

doi:10.1039/c9fo01346f

Cited by 50 publications

(36 citation statements)

References 51 publications

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“…A similar nature of research is reported by the same author, but with a different research group [63], about the effects of collagen Type I on cell migration and differentiation through the culturing of mouse C2C12 myoblasts for skeletal muscle regeneration. The collagen I increases the production of interleukin-6 using nuclear factor κB (NF-κB) p65 that results in the myogenic differentiation of C2C12 cells in the cultured assay [63].…”

Section: Biosynthesis and Culturing For Type Isupporting

confidence: 59%

Developments for Collagen Hydrolysate in Biological, Biochemical, and Biomedical Domains: A Comprehensive Review

et al. 2021

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The collagen hydrolysate, a proteinic biopeptide, is used for various key functionalities in humans and animals. Numerous reviews explained either individually or a few of following aspects: types, processes, properties, and applications. In the recent developments, various biological, biochemical, and biomedical functionalities are achieved in five aspects: process, type, species, disease, receptors. The receptors are rarely addressed in the past which are an essential stimulus to activate various biomedical and biological activities in the metabolic system of humans and animals. Furthermore, a systematic segregation of the recent developments regarding the five main aspects is not yet reported. This review presents various biological, biochemical, and biomedical functionalities achieved for each of the beforementioned five aspects using a systematic approach. The review proposes a novel three-level hierarchy that aims to associate a specific functionality to a particular aspect and its subcategory. The hierarchy also highlights various key research novelties in a categorical manner that will contribute to future research.

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Section: Biosynthesis and Culturing For Type Isupporting

confidence: 59%

Developments for Collagen Hydrolysate in Biological, Biochemical, and Biomedical Domains: A Comprehensive Review

et al. 2021

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“…There was also no difference in the growth of C2C12 on different coating substrates, suggesting that pregel solutions from different layers of the bladder did not affect the growth of C2C12 cells. Myogenesis of the C2C12 was confirmed using the following criteria: Upon initiation of differentiation, the cells elongate, adhere, and fuse into multinucleated myotubes that express muscle-specific markers such as myosin heavy chain (MyHC) and myogenic regulatory factors such as myogenin in the terminal differentiation state [25]. As can be seen from the immunocytochemistry study (Figure 7A,B), the C2C12 fused into multinucleated cells on bladder ECM-coated substrates as efficiently as on commercial collagen type I.…”

Section: Effect On C2c12 Myogenic Differentiationmentioning

confidence: 99%

Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine Bladders

et al. 2021

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Porcine urinary bladder is one of the most used organs to fabricate extracellular matrix (ECM) hydrogel. Although there are two different ECM types inside a bladder, i.e., urinary bladder matrix (UBM) and a subtype ECM (sECM), most studies have only employed UBM for hydrogel fabrication, and overlooked the potential use of sECM. In another aspect, the delamination of UBM from bladders is a time-consuming process; consequently, the use of the whole bladder (WB) will likely increase production yield. Therefore, the objective of this study was to fabricate hydrogels from sECM and WB and compare them to UBM. The results indicated that different layers of the bladder shared almost the same biochemical composition. In terms of gelation kinetics, rheology and morphology, although hydrogels from UBM and sECM exhibited some discrepancies, those from UBM and WB interestingly possessed almost the same characteristics. In in vitro studies, all the hydrogels possessed nearly the same biochemical effects towards L929 viability and C2C12 differentiation. These results could preliminarily indicate that the use of sECM should no longer be ignored, and WB could be a promising substitution for UBM hydrogels, eliminating the need for time-consuming delamination processes, as well as increasing the possibility of mass production.

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“…It was shown that high level of TNFα may cause apoptosis of myoblasts and myocytes, thus causing muscle atrophy [52]. Previous studies have shown that collagen I influences the migration and differentiation of myoblasts through regulating release of IL-6 [9]. Gelatin has also been shown to regulate production and secretion of IL-6 and TNFα in differentiated U937 lymphoma cells [18].…”

Section: Discussionmentioning

confidence: 99%

“…Fibrillar collagen I, the main component of ECM, distributes widely in endomysium, perimysium and epimysium, not only exerting biomechanical support but also influences the biological function of muscle cells [6,7,8]. Although it is known in vitro that collagen I promotes myogenesis of C2C12 myoblasts [9], muscle regeneration in vivo is known to be accompanied by a temporary and local breakdown of collagen, producing a mixture of collagen polypeptides without triple-helicity [10].…”

Section: Introductionmentioning

confidence: 99%

“…It is The copyright holder for this preprint this version posted May 26, 2021. ; https://doi.org/10.1101/2021.05. 26.445744 doi: bioRxiv preprint vitro that collagen I promotes myogenesis of C2C12 myoblasts [9], muscle regeneration in vivo is known to be accompanied by a temporary and local breakdown of collagen, producing a mixture of collagen polypeptides without triple-helicity [10].…”

Section: Introductionmentioning

confidence: 99%

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Bi-phasic effect of gelatin in myogenesis and skeletal muscle regeneration

Liu

Chang

et al. 2021

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Skeletal muscle regeneration requires extracellular matrix (ECM) remodeling including an acute and transient breakdown of collagen that produces gelatin. However, the physiological function of such a remodeling process on muscle tissue repair is unclear. Here we elaborate on a bi-phasic effect of gelatin in skeletal muscle regeneration, mediated by hormetic effects of reactive oxygen species (ROS). Low-dose gelatin stimulates ROS production from NADPH oxidase 2 (NOX2) and simultaneously upregulates antioxidant system for cellular defense, reminiscent of the adaptive compensatory process during mild stress. This response triggers the release of myokine IL-6 which stimulates myogenesis and facilitates muscle regeneration. By contrast, high-dose gelatin stimulates ROS overproduction from NOX2 and mitochondrial chain complex, and ROS accumulation by suppressing antioxidant system, triggering release of TNFα, which inhibits myogenesis and regeneration. Our findings reveal gelatin-ROS-IL-6/TNFα signaling cascades underlying a hormetic response of myogenic cells to gelatin.

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Type I collagen promotes the migration and myogenic differentiation of C2C12 myoblastsviathe release of interleukin-6 mediated by FAK/NF-κB p65 activation

Abstract: Type I collagen has the potential to promote the migration and differentiation of C2C12 myoblast via IL-6 release that was mediated by FAK/NF-κB pathway.

Cited by 50 publications

References 51 publications

Developments for Collagen Hydrolysate in Biological, Biochemical, and Biomedical Domains: A Comprehensive Review

Developments for Collagen Hydrolysate in Biological, Biochemical, and Biomedical Domains: A Comprehensive Review

Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine Bladders

Bi-phasic effect of gelatin in myogenesis and skeletal muscle regeneration

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