Type I collagen production by osteoblast‐like cells cultured in contact with different bioactive glasses

Bosetti, Michela; Zanardi, Laura; Hench, Larry L.; Cannas, M.

doi:10.1002/jbm.a.10415

Cited by 108 publications

(64 citation statements)

References 27 publications

(34 reference statements)

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“…Yao et al [46] noted that the dissolution products from poly(lactic-co-glycolic)/bioactive glass composite scaffold stimulated osteogenesis of rat marrow stromal cells, as was confirmed by increased mRNA expression of osteoblastic markers such as osteocalcin, alkaline phosphatase and bone sialoprotein. In another work [47], human primary osteoblast-like cells cultured in contact with melt-derived 45S5, as well as gel-derived 58S and 77S bioactive glasses revealed different behaviour, including ALP activity, as well as collagen type I expression and synthesis. The authors pointed out that the 45S5 glass significantly increased collagen secretion and ALP activity, compared to the other two gel-derived bioactive glasses, which can be related to alkalization of the culture medium.…”

Section: In Vitro Osteoblast Responsementioning

confidence: 95%

A new insight into in vitro behaviour of poly(ε-caprolactone)/bioactive glass composites in biologically related fluids

et al. 2017

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In the present work, the role of content, size and chemical composition of gel-derived bioactive glass particles from the SiO 2 -CaO-P 2 O 5 system in modulating the in vitro bioactivity, osteoinductive properties and long-term (up to 15 months) degradation behaviour of poly(e-caprolactone)-based composite films was investigated. Bioactivity was assessed in simulated body fluid (SBF) and HEPES-free Dulbecco's modified Eagle medium (DMEM) supplemented with 10% foetal bovine serum (FBS), while hydrolytic degradation tests were performed in phosphate buffer saline. Obtained composite films showed excellent calcium phosphate (CaP) layer forming ability in both SBF and DMEM-10% FBS. However, kinetics of bioactivity process strongly depended on the type of medium used. The layer of amino acids and proteins, derived from cell culture medium, on the surfaces of composites created barrier that inhibited release of the ions on the one hand, while increasing nucleation density of calcium phosphates, affecting the morphology of formed CaP layers on the other. The presence of bioactive glass fillers was shown to impart osteoinductive properties to obtained films, supporting osteoblast attachment and proliferation, as well as stimulating cell differentiation and also matrix mineralization process in vitro. We showed that kinetics of bioactivity process and also osteoinductive properties of composite films could be easily modulated with the use of different contents and chemical compositions of fillers. The results showed that modification of PCL matrix with bioactive glass particles accelerated its degradation. We proved that the degradation rate of composites could be controlled and optimized for bone regeneration, in particular by using bioactive fillers causing different calcium phosphate layer forming ability on the surfaces of composites, depending on particle size and chemical composition. We have presented new opportunities to design and obtain multifunctional composites with tunable degradation and bioactivity kinetics, as well as biological properties that can meet complex requirements of bone tissue engineering.

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Section: In Vitro Osteoblast Responsementioning

confidence: 95%

A new insight into in vitro behaviour of poly(ε-caprolactone)/bioactive glass composites in biologically related fluids

et al. 2017

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“…Indeed, numerous in vivo and in vitro studies have shown that 45S5 Bioglass ® can stimulate bone regeneration (Bosetti et al 2003;Gough et al 2004;Livingston et al 2002;Loty et al 2001;Tsigkou et al 2007;Xynos et al 2000). As a result, Bioglass ® is said to be both osteoproductive and osteoconductive.…”

Section: Introductionmentioning

confidence: 99%

Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells

Kirkham

et al. 2014

Cell Tissue Res

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Translational research in bone tissue engineering is essential for 'bench to bedside' patient benefit. However, the idea combination of stem cells and biomaterial scaffolds for bone repair/regeneration is still unclear. The aim of this study was to investigate the osteogenic capacity of a combination of poly(DL-lactic acid) (PDLLA) porous foams containing 5 and 40 wt% of Bioglass ® particles with human adipose-derived stem cells (ADSCs) in vitro and in vivo. Live/dead fluorescent markers, confocal microscopy and scanning electron microscopy (SEM) showed that PDLLA/Bioglass ® porous scaffolds supported ADSCs attachment, growth and osteogenic differentiation, which were confirmed by enhanced alkaline phosphatase activity (ALP). Higher Bioglass ® content of the PDLLA foams increased more ALP activity compared to PDLLA only group. Extracellular matrix deposition after 8 weeks in in vitro culture was evident by Alcian blue/Sirius red staining.In vivo bone formation was assessed using scaffold/ADSCs constructs in diffusion chambers transplanted intraperitoneally into nude mice and recovered after 8 weeks.Histological and immunohistochemical assays indicated significant new bone formation in the 40 wt% and 5 wt% Bioglass ® constructs compared to the PDLLA only group. This study indicated the combination of a well-developed biodegradable bioactive porous PDLLA/Bioglass ® composite scaffold with a high potential stem cells source -human ADSCs could be a promising approach for bone regeneration in clinical setting.

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“…BG 13-93 is biodegradable, and when placed in an aqueous environment at 37°C, it converts to a calcium phosphate material analogous to hydroxyapatite [20,26]. As BG degrades, it also releases its ion content, which has the ability to stimulate osteoblast activity [3,4,40,42]. Previous studies have shown, in an osteoblast cell line, increasing the pH can improve collagen Type I production by increasing cellular metabolism [10,42].…”

Section: Discussionmentioning

confidence: 99%

“…Our work with an alternative silicate-based glass composition, Bioactive Glass 13-93 (BG 13-93), shows this composition can be readily shaped into porous constructs of complex shapes, while retaining the desirable in vitro bioactive characteristics of 45S5 glass and higher mechanical strength [16,32]. It is known, as BG degrades, it also releases its ion content, which has the ability to stimulate osteoblast activity [3,4,40,42]. However, there is currently a lack of studies investigating the effect of BG 13-93 on chondrocyte behavior and its use in cartilage tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Bioactive Glass 13-93 as a Subchondral Substrate for Tissue-engineered Osteochondral Constructs: A Pilot Study

Jayabalan

Tan

Rahaman

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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Background Replacement of diseased areas of the joint with tissue-engineered osteochondral grafts has shown potential in the treatment of osteoarthritis. Bioactive glasses are candidates for the osseous analog of these grafts. Questions/purposes (1) Does Bioactive Glass 13-93 (BG 13-93) as a subchondral substrate improve collagen and glycosaminoglycan production in a tissue-engineered cartilage layer? (2) Does BG 13-93 as a culture medium supplement increase the collagen and glycosaminoglycan production and improve the mechanical properties in a tissue-engineered cartilage layer?Methods In Study 1, bioactive glass samples (n = 4) were attached to a chondrocyte-seeded agarose layer to form an osteochondral construct, cultured for 6 weeks, and compared to controls. In Study 2, bioactive glass samples (n = 5) were cocultured with cell-seeded agarose for 6 weeks. The cell-seeded agarose layer was exposed to BG 13-93 either continuously or for the first or last 2 weeks in culture or had no exposure. Results Osteochondral constructs with a BG 13-93 base had improved glycosaminoglycan deposition but less collagen II content. Agarose scaffolds that had a temporal exposure to BG 13-93 within the culture medium had improved mechanical and biochemical properties compared to continuous or no exposure. Conclusions When used as a subchondral substrate, BG 13-93 did not improve biochemical properties compared to controls. However, as a culture medium supplement, BG 13-93 improved the biochemical and mechanical properties of a tissue-engineered cartilage layer. Clinical Relevance BG 13-93 may not be suitable in osteochondral constructs but could have potential as a medium supplement for neocartilage formation.

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Type I collagen production by osteoblast‐like cells cultured in contact with different bioactive glasses

Cited by 108 publications

References 27 publications

A new insight into in vitro behaviour of poly(ε-caprolactone)/bioactive glass composites in biologically related fluids

A new insight into in vitro behaviour of poly(ε-caprolactone)/bioactive glass composites in biologically related fluids

Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells

Bioactive Glass 13-93 as a Subchondral Substrate for Tissue-engineered Osteochondral Constructs: A Pilot Study

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