Type I collagen carboxyterminal telopeptide in human gingival crevicular fluid in different clinical conditions and after periodontal treatment

Abstract

A total of 126 gingival crevicular fluid (GCF) samples were collected from 20 adults using paper strips. Patients were divided into a periodontitis-affected group (13 subjects) and a periodontitis-free group (7 subjects) by pocket depth and radiological bone loss. 4 subjects from the periodontitis-affected group received a single episode of periodontal treatment (scaling, root planing and curettage) and GCF samples were collected 2, 5, 10, 20 and 40 days after treatment. Type I collagen carboxyterminal telopeptide (ICTP) in GCF was extracted into saline solution and determined by a radioimmunological method. Mean GCF ICTP concentration was 425 micrograms/l (SEM 45) in periodontitis patients and 148 micrograms/l (SEM 25) in periodontitis-free subjects, i.e., GCF ICTP concentrations were about 100 x higher than serum reference values. Significant positive correlations were found between GCF ICTP total amount per site and plaque index (R = 0.362), papilla bleeding index (R = 0.259), pocket depth (R = 0.464) and radiological bone loss (R = 0.418). Periodontal treatment decreased GCF ICTP concentration to the level seen in healthy subjects. However, large variations were seen between subjects and sites. ICTP levels below the detection limit were often found in deep pockets, as well as high values in periodontitis-free subjects. It was concluded that GCF ICTP reflects the local type I collagen degradation in periodontal tissues, and probably gives information about the tissue destruction process beyond the reach of the clinical parameters.

Relationship between C‐telopeptide pyridinoline cross‐links (ICTP) and putative periodontal pathogens in periodontitis

“…Therefore, ICTP may be a first generation pyridinoline cross-link detectable in GCF. Newer methods may involve the use of deoxypyridinoline and other cross-links as immunodetection methods are optimized.The results of this investigation extend previous studies (Giannobile et al 1995, Talonpoika & Hämäläinen 1994) that demonstrated a relationship between ICTP and inflammatory periodontal disease. Shibutani et al (1997) showed similar findings with deoxypyridinoline (found most abundantly in bone and dentin) measured in the GCF, serum and urine during experimental periodontitis in dogs.…”

“…These investigations have shown that ICTP correlated strongly with radiographic bone level and pocket depth and was significantly higher at periodontitis sites compared to non-periodontitis sites (Talonpoika & Hämäläinen 1994). In a ligature-induced experimental periodontitis study in dogs, the GCF levels of pyridinoline cross-links (ICTP and deoxypyridinoline) significantly increased during the development of attachment loss and osteoclastic bone resorption (Giannobile et al 1995, Shibutani et al 1997.…”

“…These diagnostics are effective in determining disease status, but are poor predictors of future disease activity (Haffajee et al 1983. Recent advances in the diagnosis of metabolic skeletal disorders have lead to the investigation of markers of periodontal alveolar bone loss (Giannobile 1997 Hämäläinen 1994,Giannobile et al 1995, Shibutani et al 1997) and peri-implantitis (Oringer et al 1998).…”

“…These diagnostics are effective in determining disease status, but are poor predictors of future disease activity (Haffajee et al 1983. Recent advances in the diagnosis of metabolic skeletal disorders have lead to the investigation of markers of periodontal alveolar bone loss (Giannobile 1997 Hämäläinen 1994,Giannobile et al 1995, Shibutani et al 1997) and peri-implantitis (Oringer et al 1998).Pyridinoline cross-linked carboxyterminal telopeptide of type I collagen (ICTP) is derived from the carboxyterminal telopeptide regions of type I collagen cross-linked via pyridinoline or deoxypyridinoline . Following procollagen synthesis and its release into the maturing extracellular matrix, collagen fibrils undergo post-translational modification resulting in cross-links between the telopeptide regions of type I collagen chains by lysyl oxidase.…”

Gingival Crevicular Fluid Calprotectin, Osteocalcin and Cross-Linked N-Terminal Telopeptid Levels in Health and Different Periodontal Diseases

“…The increased recognition of the interactions between the cells of the immune and skeletal system has led to the seeking of a variety of regulatory molecules that play a role in the progression of periodontal disease [25][26][27]. In previous studies, the bestknown markers of the bone turnover have been widely investigated in GCF samples of patients with different periodontal diseases and implicated to be associated with the progression of periodontal diseases [28][29][30]. Several investigations provided contradictory evidence about the role of osteocalcin in GCF of patients with periodontitis [31][32][33].…”