Type 2 diabetes differentially affects the substrate saturation kinetic attributes of erythrocyte hexokinase and phosphofructokinase

Bhise, Sunita S; Rao, Janhavi R; Hegde, Mahabaleshwar V.; Katyare, Surendra S.

doi:10.1002/1873-3468.13604

Cited by 4 publications

(3 citation statements)

References 28 publications

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“…Diabetic group showed damage in the cells because of mild deterioration in the pancreatic nucleus and β-cells. This occurred due to conditions of hyperglycemia causes pancreatic cells to diminish insulin function [15]. Furthermore, the hyperglycemic condition can also cause oxidative stress, because of increased free radical generation.…”

Section: Resultsmentioning

confidence: 99%

Antidiabetic Effect of Katakakhadiradi kashayam by Improving the Insulin Expression and Glucose Metabolising Enzyme

Subbiah

Kavimani

Ramadoss

et al. 2021

JPRI

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Many plants provide a rich source of bioactive chemicals, which are free from undesirable side effects and possess powerful pharmacological actions. The present study was carried out to find the antidiabetic effect of Katakakhadiradi kashayam (KKK) by improving the insulin expression and regulating properly the glucose metabolising enzymes. The diabetes was induced in combination with streptozotocin and nicotinamide injection to Wistar rats. Diabetic rats were treated with Katakakhadiradi kashayam orally at doses of 100, 200 and 300 mg/kg/bw for 28 days, and the obtained results of parameters were compared with glibenclamide. The antidiabetic effect of Kashayam was measured by the expression of insulin by immunohistochemistry and restoring the normal clinical values of glucose metabolizing enzymes. The present study specified that hyperglycemia leads to pathological conditions in pancreatic tissue with decreased expression of insulin in β-cells whereas the Katakakhadiradi kashayam normalised the production of insulin. The study found that the antihyperglycemic activity of Katakakhadiradi kashayam L. is mainly due to their ability to restore the function of pancreatic tissues by causing an increase in insulin output and maintaining the glucose metabolising enzymes.

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Section: Resultsmentioning

confidence: 99%

Antidiabetic Effect of Katakakhadiradi kashayam by Improving the Insulin Expression and Glucose Metabolising Enzyme

Subbiah

Kavimani

Ramadoss

et al. 2021

JPRI

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show abstract

“…DISCUSSION: Earlier studies have shown that the enzyme make-up as well as the kinetic attributes of the key irreversible enzymes viz. hexokinase and phosphofructokinase altered significantly in Type 2 diabetic RBCs [2][3][4] . In view of this, the present studies were designed to determine whether the intra-RBC concentrations of sugar phosphate metabolites of glycolysis are also altered in the diabetic state and its implications for ATP synthesis.…”

Section: Accuracy and Precisionmentioning

confidence: 94%

Untitled

2022

IJPSR

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In previous studies, it has been shown that the activities of the glycolysis cycle enzyme are significantly altered with changes in kinetic attributes of hexokinase, phosphorfructokinase and lactate dehydrogenase. Moreover, the kinetic parameters of the terminal enzyme lactate dehydrogenase also changed significantly. In view of these observed changes, it was of interest to know if and how the flux of intermediates of the glycolytic pathway was also affected. With this objective, a simple, rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for quantification of five important glycolysis intermediates of rate-limiting steps [viz. glucose-6-phosphate (G-6-P), fructose-6phosphate (F-6-P), fructose-1,6-bis-phosphate (FBP), 3-phosphoglyceric acid (3PGA), and pyruvate in human erythrocytes. Liquid chromatography was performed using Supelco Ascentis Express RP amide (250 mm × 4.6 mm, 5µm) column with mobile phase, 10mM ammonium acetate in water and acetonitrile. The detection of metabolites was carried out by tandem mass spectrometry. In erythrocytes from Type 2 diabetic subjects, there was a significant increase in intracellular concentrations of G-6-P and 3-PGA, with a marked decrease in FBP and pyruvate. The results suggest that the metabolic flux of glycolysis is altered significantly in the erythrocytes from Type 2 diabetic patients. The significance of these findings in relation to adenosine triphosphate (ATP) synthesis and loss of membrane deformability, which lead to diabetic complications, is discussed.

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“…reducing the transmembrane concentration gradient. This phenomenon is strengthened by the subsequent saturation of the hexokinases whose Km ~50 μmol/L for D-glucose in human RBC [64], thus, limiting the D-glucose conversion to G6P in these cells [65]. The consequences of these alterations are a state of hyperglycaemia.…”

Section: The Role Of Glycolysis In D-glucose Membrane Transportmentioning

confidence: 99%

Glycaemia dynamics concepts before and after insulin

Sobrevía

2022

Biochemical Pharmacology

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Type 2 diabetes differentially affects the substrate saturation kinetic attributes of erythrocyte hexokinase and phosphofructokinase

Cited by 4 publications

References 28 publications

Antidiabetic Effect of Katakakhadiradi kashayam by Improving the Insulin Expression and Glucose Metabolising Enzyme

Antidiabetic Effect of Katakakhadiradi kashayam by Improving the Insulin Expression and Glucose Metabolising Enzyme

Untitled

Glycaemia dynamics concepts before and after insulin

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