Type 1 fimbriae in commensal Escherichia coli derived from healthy humans.

Pusz, Paweł; Bok, Ewa; Mazurek, Justyna; Stosik, Michał; Baldy-Chudzik, Katarzyna

doi:10.18388/abp.2014_1912

Cited by 18 publications

(13 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Briefly, the genes frmR and frmA of the frmRAB operon (loci ECO26_0391, ECO26_0392 and ECO26_0393 in AP010953), involved in the degradation of formaldehyde, a by-product of lignin degradation (Herring and Blattner, 2004 ), are absent in strain 36493. Some of the genes (ECO26_2103, ECO26_2104, ECO26_5529) involved in type I fimbriae synthesis (Pusz et al, 2014 ) are absent from strains 36493, 34870, 34827, 36293, and 36348. Additionally, the fimbrial genes fimA (ECO26_5511) and fimH (ECO26_5517) in strains 36493, 34870, 34827, 36293, and 36348 are more similar to the corresponding genes from O111 reference strain 11128 (99–100% nucleotide identity vs. 96–98% nucleotide identity with O26:H11 strain 11368).…”

Section: Resultsmentioning

confidence: 99%

The Mobilome; A Major Contributor to Escherichia coli stx2-Positive O26:H11 Strains Intra-Serotype Diversity

et al. 2017

View full text Add to dashboard Cite

Shiga toxin-producing Escherichia coli of serotype O26:H11/H- constitute a diverse group of strains and several clones with distinct genetic characteristics have been identified and characterized. Whole genome sequencing was performed using Illumina and PacBio technologies on eight stx2-positive O26:H11 strains circulating in France. Comparative analyses of the whole genome of the stx2-positive O26:H11 strains indicate that several clones of EHEC O26:H11 are co-circulating in France. Phylogenetic analysis of the French strains together with stx2-positive and stx-negative E. coli O26:H11 genomes obtained from Genbank indicates the existence of four clonal complexes (SNP-CCs) separated in two distinct lineages, one of which comprises the “new French clone” (SNP-CC1) that appears genetically closely related to stx-negative attaching and effacing E. coli (AEEC) strains. Interestingly, the whole genome SNP (wgSNP) phylogeny is summarized in the cas gene phylogeny, and a simple qPCR assay targeting the CRISPR array specific to SNP-CC1 (SP_O26-E) can distinguish between the two main lineages. The PacBio sequencing allowed a detailed analysis of the mobile genetic elements (MGEs) of the strains. Numerous MGEs were identified in each strain, including a large number of prophages and up to four large plasmids, representing overall 8.7–19.8% of the total genome size. Analysis of the prophage pool of the strains shows a considerable diversity with a complex history of recombination. Each clonal complex (SNP-CC) is characterized by a unique set of plasmids and phages, including stx-prophages, suggesting evolution through separate acquisition events. Overall, the MGEs appear to play a major role in O26:H11 intra-serotype clonal diversification.

show abstract

Section: Resultsmentioning

confidence: 99%

The Mobilome; A Major Contributor to Escherichia coli stx2-Positive O26:H11 Strains Intra-Serotype Diversity

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Incompleteness of this operon is rather common in commensal E. coli from pigs, reaching 44.9% and 66.9% among isolates from piglets and sows, respectively. The previous studies indicated that many human commensal isolates positive for the fimH gene showed a lack of type 1 fimbriae due to the deletion or lack of the expression of one or more of the type 1 fimbriae operon genes [63]. Such results suggest that the fimH gene is not a good marker of type 1 fimbriae, much better would be the fimA gene.…”

Section: Discussionmentioning

confidence: 99%

Extended Phylogeny and Extraintestinal Virulence Potential of Commensal Escherichia coli from Piglets and Sows

Bok

Kożańska

Mazurek-Popczyk

et al. 2020

IJERPH

Self Cite

View full text Add to dashboard Cite

Commensal Escherichia coli, naturally occurring in the intestinal tract, can be the origin of extraintestinal pathogenic E. coli (ExPEC) strains. ExPEC causes high mortality and significant economic losses in the swine industry in several countries and poses a serious threat to public health worldwide. The aim of this study was to analyze the extended phylogenetic structure and extraintestinal virulence potential in two groups of commensal E. coli isolates from post-weaning piglets and sows. The phylogenetic assignment to eight groups was determined using the revised Clermont phylogenetic typing method in quadruplex PCR. Identification of extraintestinal virulence genes (VGs) and adhesin operon genes was performed using multiplex or simplex PCR. The revised phylogenetic assignment allowed us to distinguish E. coli with significantly higher (groups C and F) or lower (group E) virulence potential in isolates from piglets. The majority of the tested VGs occurred more frequently in isolates from piglets than from sows, with statistically significant differences for seven genes: fimH, papAH, iutA, iroN, ompT, traT, and iss. Complete operons for type I and P fimbriae significantly prevailed among E. coli from piglets. This study provides insight into the extended phylogenetic structure of porcine commensal E. coli and showed that these strains, particularly from piglets, constitute a considerable reservoir of extraintestinal VGs and may increase the potential risk of extraintestinal infections.

show abstract

“…E. coli produce many types of fimbriae, of which type 1 fimbriae often occur on their surface. The type 1 fimbriae are encoded by a fim gene cluster, including nine genes required for its biosynthesis (Pusz et al 2014 ). We screened for the presence of fimA gene encoding the major subunit of type 1 fimbriae and the presence of fimH gene that determines the biosynthesis of the specific adhesin of these fimbriae.…”

Section: Discussionmentioning

confidence: 99%

Virulence-associated genes and antibiotic susceptibility among vaginal and rectal Escherichia coli isolates from healthy pregnant women in Poland

et al. 2018

View full text Add to dashboard Cite

Vaginal and/or rectal Escherichia coli colonization of pregnant women is sometimes associated with neonatal infections. Despite the relevance of these strains, they have been rarely described before. Thus, the aim of this study was to compare vaginal (VEC) and rectal E. coli (REC) isolates in respect of antimicrobial susceptibility and the frequency of virulence-associated genes (VAGs). The antimicrobial susceptibility of 50 VEC and 50 REC isolates was performed by using the disc diffusion method, and VAGs were detected by PCR. There were no significant differences in the antimicrobial resistance between VEC and REC. Both VEC and REC isolates were mostly resistant to ticarcillin (36 and 30%) and ampicillin (36 and 22%). None of the tested isolates was positive for ESBL. Gene’s fimH, fimA, sfa/foc, iutA, ibeA, hlyF, and neuC were detected, respectively, in 98, 92, 32, 28, 12, 8, and 2% of VEC and in 94, 72, 12, 34, 8, 10, and 8% of REC isolates. The co-occurrence of fimA/H and sfa/foc genes was significantly more prevalent among VEC isolates, in comparison to REC isolates. The study indicated that VEC and REC isolates are quite similar in terms of antimicrobial non-susceptibility and VAGs.

show abstract

Type 1 fimbriae in commensal Escherichia coli derived from healthy humans.

Cited by 18 publications

References 8 publications

The Mobilome; A Major Contributor to Escherichia coli stx2-Positive O26:H11 Strains Intra-Serotype Diversity

The Mobilome; A Major Contributor to Escherichia coli stx2-Positive O26:H11 Strains Intra-Serotype Diversity

Extended Phylogeny and Extraintestinal Virulence Potential of Commensal Escherichia coli from Piglets and Sows

Virulence-associated genes and antibiotic susceptibility among vaginal and rectal Escherichia coli isolates from healthy pregnant women in Poland

Contact Info

Product

Resources

About