Virulence-associated genes and antibiotic susceptibility among vaginal and rectal Escherichia coli isolates from healthy pregnant women in Poland

Abstract

Vaginal and/or rectal Escherichia coli colonization of pregnant women is sometimes associated with neonatal infections. Despite the relevance of these strains, they have been rarely described before. Thus, the aim of this study was to compare vaginal (VEC) and rectal E. coli (REC) isolates in respect of antimicrobial susceptibility and the frequency of virulence-associated genes (VAGs). The antimicrobial susceptibility of 50 VEC and 50 REC isolates was performed by using the disc diffusion method, and VAGs were detected by PCR. There were no significant differences in the antimicrobial resistance between VEC and REC. Both VEC and REC isolates were mostly resistant to ticarcillin (36 and 30%) and ampicillin (36 and 22%). None of the tested isolates was positive for ESBL. Gene’s fimH, fimA, sfa/foc, iutA, ibeA, hlyF, and neuC were detected, respectively, in 98, 92, 32, 28, 12, 8, and 2% of VEC and in 94, 72, 12, 34, 8, 10, and 8% of REC isolates. The co-occurrence of fimA/H and sfa/foc genes was significantly more prevalent among VEC isolates, in comparison to REC isolates. The study indicated that VEC and REC isolates are quite similar in terms of antimicrobial non-susceptibility and VAGs.

Phylogenicity and Virulence Profiles of Clinical Escherichia coli Isolates in the Ho Teaching Hospital of Ghana

“…These genes were detected in 88.9%, 77.0%, and 27.4% of the E. coli isolates, respectively. Similar results were reported in other parts of the world for sfa/foc genes [3,23] and fimA genes [24,25]. These plasmid-encoded fimA genes were commonly found in isolates from infection at the lower urinary tract, where they adhere to the urethral mucosa epithelial cells [24].…”

Investigation of Antibiotic Susceptibility and Virulence Genes in Escherichia coli Strains Isolated from Blood and Urine Samples

“…A total of 150 E. coli strains isolated from blood (50) and urine (100) samples sent from various departments (i.e., pediatric [32], urology [19], emergency [12], oncology [10], infectious diseases [9], reanimation intensive care [9], nephrology [9], hematology [8], neurology [7], gynecology and obstetrics [6], general surgery [6], and other clinics [23]) of the hospital to the Microbiology Laboratory of the Selçuk University Faculty of Medicine Hospital were included in the study. Only one isolate per patient was included in the research.…”

“…Primer sequences, cycling conditions, and base pairs of target genes in every one of the mixes are presented in Table 1 . 3 9 10 11 12 13 Each mix was adapted, taking the PCR cycle (especially annealing) and primer lengths into account. Each multiplex PCR was checked once with a single PCR and the multiplexes continued when no problem were encountered.…”

Probiotic bacteria and bile acid profile are modulated by prebiotic diet and associate with facilitated diurnal clock/sleep realignment after chronic disruption of rhythms

“…Because our study included both prebiotic and control diet conditions, this time course enabled us to control for changes in the microbiome and bile acid profiles due to the rat age, surgery, and time in the vivarium. This is important because each of these factors can impact the fecal microbiome and bile acid profile (Yatsunenko et al 2012; Perino et al 2020; Keskey et al 2020; Ferrie et al 2020; Kaczmarek et al 2018).…”

“…The copyright holder for this preprint this version posted March 4, 2021. ; https://doi.org/10.1101/2021.03.03.433775 doi: bioRxiv preprint is important because each of these factors can impact the fecal microbiome and bile acid profile (Yatsunenko et al 2012;Perino et al 2020;Keskey et al 2020;Ferrie et al 2020;Kaczmarek et al 2018).…”