Type 1 and Type 2 cytokines imbalance in adult male C57BL/6 mice following a 7-day oral exposure to perfluorooctanesulfonate (PFOS)

Zheng, Li; Dong, Guang‐Hui; Zhang, Yinghua; Liang, Zhenglun; Jin, Yinlong; He, Qincheng

doi:10.3109/1547691x.2010.537287

Cited by 56 publications

(43 citation statements)

References 37 publications

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“…These results were consistent with similar observations in previous studies evaluating PFOS, perfluoroctanic acid (PFOA), and perfluorinated insecticide N-ethy1-perfluorooctane sulfonamide (N-EtPFOSA) (PedenAdams et al, 2008;Qazi et al, 2009;Kim et al, 2011;Zheng et al, 2011;Elcombe et al, 2012). For example, a recent study by Elcombe et al (2012) evaluated PFOSinduced liver-related effects in male Sprague Dawley rats; following a 7-day dietary exposure to K+PFOS at 20 or 100 ppm, there was a significantly increased liver weight.…”

Section: Discussionsupporting

confidence: 90%

“…Details of the analytical procedure to measure PFOS in each serum sample have been provided previously (Hansen et al, 2001;Zheng et al, 2011). Briefly, 0.5 ml of serum, 1 ml of 0.5 M tetrabutyl-ammonium hydrogen sulfate solution, and 2 ml of sodium carbonate buffer (0.25 M, pH 10) were added to 15 ml polypropylene tube and thoroughly mixed.…”

Section: Serum Pfos Analysismentioning

confidence: 99%

“…The sample was then passed through a nylon filter (Autovial R5 PUNYL; 0.45-µm pore size; Whatman, Tokyo) to remove any suspended materials and insoluble particles. Each extracted solution was then analyzed by liquid chromatography-mass spectrometry (LC/MS system, Shimadzu, Kyoto, Japan) as previously described (Jin et al, 2007;Zheng et al, 2011).…”

Section: Serum Pfos Analysismentioning

confidence: 99%

“…Reports concerning the immunotoxicity of PFOS in vitro and in vivo have appeared in the literature (Keil et al, 2008;Lefebvre et al, 2008;Peden-Adams et al, 2008;Dong et al, 2009;Qazi et al, 2009;Brieger et al, 2011;Mollenhauer et al, 2011;Wang et al, 2011;Zheng et al, 2011;Corsini et al, 2012;Dewitt et al, 2012). However, there is still a lack of understanding of the toxicokinetics and mode(s)/mechanism(s) of immunotoxic action of PFOS.…”

Section: Introductionmentioning

confidence: 99%

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Mechanism of perfluorooctanesulfonate (PFOS)-induced apoptosis in the immunocyte

Zhang

Wang

Dong

et al. 2012

Journal of Immunotoxicology

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Section: Discussionsupporting

confidence: 90%

Section: Serum Pfos Analysismentioning

confidence: 99%

Section: Serum Pfos Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mechanism of perfluorooctanesulfonate (PFOS)-induced apoptosis in the immunocyte

Zhang

Wang

Dong

et al. 2012

Journal of Immunotoxicology

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“…Exposure of laboratory animals to PFOA and PFOS has resulted in hepatomegaly, hepatic peroxisome proliferation, atrophy and alteration of cell populations in lymphoid organs (Yang et al 2000;Qazi et al 2009;Son et al 2009;DeWitt et al 2012;Dong et al 2012), suppression of the T-cell-dependent IgM antibody response (TDAR), alterations in inflammatory cytokine signaling (Peden-Adams et al 2008;Zheng et al 2009Zheng et al , 2011Dong et al 2011;DeWitt et al 2012), and reduction in thyroid hormone levels (Curran et al 2008). PFOS reduced the host resistance to influenza A virus and natural killer cell activity in B 6 C 3 F 1 mice (Guruge et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Immunotoxic and hepatotoxic effects of perfluoro-n-decanoic acid (PFDA) on female Harlan Sprague–Dawley rats and B₆C₃F₁/N mice when administered by oral gavage for 28 days

Frawley

Smith

Cesta

et al. 2018

Journal of Immunotoxicology

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Poly-and perfluoroalkyl substances (PFAS) are chemically and thermally stable, hydrophobic, lipophobic compounds used in stain repellants and water and oil surfactants, and associated with immunosuppression and peroxisome proliferator activity. Perfluoro-n-decanoic acid (PFDA, (CF 3 (CF 2 ) 8 COOH), a fluorinated straight chain fatty acid compound, is reported to induce thymic atrophy and reversible bone marrow hypocellularity in rodent models. The objective of this study was to assess potential immunotoxicity of PFDA, due to its structural similarity to other immunosuppressive PFASs. Female Harlan Sprague-Dawley rats were exposed to 0-2.0 mg PFDA/kg by oral gavage daily for 28 d. Female B 6 C 3 F 1 /N mice were exposed once/week to 0-5.0 mg PFDA/kg by gavage for 4 weeks. Animals were evaluated for effects on immune cell populations in spleen and bone marrow, and innate, humoral-, and cell-mediated immunity. Mice were also evaluated for resistance to Influenza virus. Treatment-related hepatocyte necrosis and hepatomegaly were observed in rats treated with 0.5 mg PFDA/kg/d. In mice, hepatomegaly (26-89%) was observed following exposure to !0.625 mg PFDA/kg/week, while splenic atrophy (20%) was observed at 5.0 mg PFDA/kg/week. At 5.0 mg PFDA/kg/week, total spleen cells, and Ig þ and NK þ cells were decreased (17.6-27%). At ! 1.25 mg PFDA/kg/week the numbers of splenic CD3 þ , CD4 þ , CD8 þ , and Mac3 þ cells were decreased (10.5-39%). No changes were observed in leukocyte subpopulations in PFDA-exposed rats. Phagocytosis by fixed-tissue macrophages was decreased in liver (specific activity, 24-39%) at !0.25 mg PFDA/kg/d in rats. PFDA-induced effects on humoral-and cell-mediated immunity, host resistance, and bone marrow progenitor cells were limited. These data suggest that exposure to PFDA may induce adverse effects in rat liver in a manner consistent with the PFAS class, and may also alter the balance of immune cell populations in lymphoid tissues in mice.ARTICLE HISTORY

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In vitro evaluation of the effects of perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) on IL‐2 production in human T‐cells

Midgett

Peden‐Adams

Gilkeson

et al. 2014

J of Applied Toxicology

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Perfluorinated compounds, such as perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), have been shown to alter various immune functions suggesting they are immunotoxic. This study assessed the effects of PFOS and PFOA on interleukin (IL)-2 production in the human Jurkat T-cell line and PFOS in healthy human primary T cells. Jurkat cells were stimulated with phytohemagglutinin (PHA)/phorbol myristate acetate (PMA), anti CD-3/anti CD-28, or anti CD-3, and dosed with 0, 0.05, 0.1, 0.5, 1, 5, 10, 50, 75, or 100 μg ml−1 PFOS or 0, 0.005, 0.01, 0.05, 0.1, 0.5, 1, 5, or 10 μg ml−1 PFOA. Jurkat cells stimulated with PHA/PMA or anti CD-3 exhibited decreased IL-2 production beginning at 50 μg PFOS ml−1 and 5 μg PFOS ml−1 respectively, but stimulation with anti-CD3/anti-CD28 resulted in no changes compared with the control. Addition of the PPAR-alpha antagonist GW6471 to PFOS-dosed cells stimulated with PHA/PMA resulted in decreases in IL-2 production starting at 50 μg PFOS ml−1, which suggests PFOS affected T-cell IL-2 production via PPAR-alpha-independent mechanisms. Exposure to PFOA, PFOA + GW6471, or PFOS + PFOA in Jurkat cells resulted in no significant differences in IL-2 production. In vitro dosing studies using healthy primary human CD4+ T cells were consistent with the Jurkat results. These data demonstrated that PFOA did not impact IL-2 production, but PFOS suppressed IL-2 production in both a human cell line and human primary cells at dose levels within the high end of the human exposure range. A decrease in IL-2 production is characteristic of autoimmune diseases such as systemic lupus erythematosus and should be further investigated.

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Type 1 and Type 2 cytokines imbalance in adult male C57BL/6 mice following a 7-day oral exposure to perfluorooctanesulfonate (PFOS)

Cited by 56 publications

References 37 publications

Mechanism of perfluorooctanesulfonate (PFOS)-induced apoptosis in the immunocyte

Mechanism of perfluorooctanesulfonate (PFOS)-induced apoptosis in the immunocyte

Immunotoxic and hepatotoxic effects of perfluoro-n-decanoic acid (PFDA) on female Harlan Sprague–Dawley rats and B₆C₃F₁/N mice when administered by oral gavage for 28 days

In vitro evaluation of the effects of perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) on IL‐2 production in human T‐cells

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Type 1 and Type 2 cytokines imbalance in adult male C57BL/6 mice following a 7-day oral exposure to perfluorooctanesulfonate (PFOS)

Cited by 56 publications

References 37 publications

Mechanism of perfluorooctanesulfonate (PFOS)-induced apoptosis in the immunocyte

Mechanism of perfluorooctanesulfonate (PFOS)-induced apoptosis in the immunocyte

Immunotoxic and hepatotoxic effects of perfluoro-n-decanoic acid (PFDA) on female Harlan Sprague–Dawley rats and B6C3F1/N mice when administered by oral gavage for 28 days

In vitro evaluation of the effects of perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) on IL‐2 production in human T‐cells

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Product

Resources

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Immunotoxic and hepatotoxic effects of perfluoro-n-decanoic acid (PFDA) on female Harlan Sprague–Dawley rats and B₆C₃F₁/N mice when administered by oral gavage for 28 days