Tylosin effect on methanogenesis in an anaerobic biomass from swine wastewater treatment

Abstract

The effect of different concentrations of tylosin on methane production was investigated: first methanogenesis in a biomass without contact with the antibiotic, and later the ability of the sludge to adapt to increasing concentrations of tylosin. Results showed that, for biomass that had no contact with the antibiotic, the presence of tylosin inhibits the generation of methane even at concentrations as small as 0.01 mg L(-1), and samples at concentrations above 0.5 mg L(-1) produced practically no methane, whereas, in the digesters acclimated in the presence of tylosin at a concentration of 0.01 to 0.065 mg L(-1), methanogenesis is not inhibited in the presence of antibiotic and the generation of methane is improved. This behaviour suggests the microorganisms have developed not only resistance to the antibiotic but also an ability to metabolize it.

Antibiotic Residues in Animal Waste: Occurrence and Degradation in Conventional Agricultural Waste Management Practices

“…In general, the preponderance of the literature [49,55,74,96,[101][102][103][104][105][106][107][108][109][110][111][112][113] focuses on the effects of antibiotics on the anaerobic digestion process rather than the fate of antibiotics during treatment. Individual antibiotics demonstrate a range of impacts on biogas production, from no effect to complete inhibition.…”

Bioprocessing for elimination antibiotics and hormones from swine wastewater

“…The microorganisms had not only developed resistance to the antibiotics, but also the ability to metabolize them (García-Sánchez et al, 2016). Huang et al (2014a) and Huang et al (2014b) investigated the effects of different antibiotic addition methods (added antibiotics to reactor directly/ added the polluted pig manure to reactor) on methane production from the anaerobic digestion of swine wastes.…”

Effect of Antibiotics on the Microbial Efficiency of Anaerobic Digestion of Wastewater: A Review

“…Chelliapan et al (2011) showed an adaption of methanogens to tylosin, where archaeal cells were not inhibited by tylosin at the concentrations between 100 and 400 mg/L and dominated the reactor. Especially, methanogenesis was not inhibited in the digesters acclimated with 0.01 to 0.065 mg/L tylosin, and methane production was increased (Garcia-Sanchez et al, 2016). It could be inferred that not only the resistance but also the metabolizing ability to antibiotics were developed by such microorganisms.…”

“…Adaptation may also occur. Indeed, a low concentration of tylosin, 0.01 mg/L was enough to inhibit methane production for biomass that has not been previously in contact with tylosin; whereas no inhibition of methanogenesis was observed in digesters acclimated with 0.01-0.065 mg/L of tylosin (Garcia-Sanchez et al, 2016). Overall, macrolides decrease methane production by about 10-100% depending on the antibiotic concentration (Table 3).…”