Two‐way and three‐way approaches to ultra high performance liquid chromatography–photodiode array dataset for the quantitative resolution of a two‐component mixture containing ciprofloxacin and ornidazole

Abstract

Two-way and three-way calibration models were applied to ultra high performance liquid chromatography with photodiode array data with coeluted peaks in the same wavelength and time regions for the simultaneous quantitation of ciprofloxacin and ornidazole in tablets. The chromatographic data cube (tensor) was obtained by recording chromatographic spectra of the standard and sample solutions containing ciprofloxacin and ornidazole with sulfadiazine as an internal standard as a function of time and wavelength. Parallel factor analysis and trilinear partial least squares were used as three-way calibrations for the decomposition of the tensor, whereas three-way unfolded partial least squares was applied as a two-way calibration to the unfolded dataset obtained from the data array of ultra high performance liquid chromatography with photodiode array detection. The validity and ability of two-way and three-way analysis methods were tested by analyzing validation samples: synthetic mixture, interday and intraday samples, and standard addition samples. Results obtained from two-way and three-way calibrations were compared to those provided by traditional ultra high performance liquid chromatography. The proposed methods, parallel factor analysis, trilinear partial least squares, unfolded partial least squares, and traditional ultra high performance liquid chromatography were successfully applied to the quantitative estimation of the solid dosage form containing ciprofloxacin and ornidazole.

Molecularly imprinted polymer based diffusive gradients in thin‐films for in situ selective sampling and determination of ciprofloxacin

“…A series of analytical methods have been reported for the determination of CF including spectrophotometry, mass spectrophotometry, liquid chromatography, high performance liquid chromatography, capillary electrophoresis, and electrochemical techniques [6][7][8][9][10][11][12]. The free concentration is generally believed to be responsible for the toxic effects and bioavailable for organisms [13].…”

Second‐order calibration serves as a remedial measure for the simultaneous determination of andrographolide and dehydroandrographolide in Andrographis paniculata and its preparations by HPLC without complete baseline separation

“…This appealing feature, named “second‐order advantage” , thus nicely caters for the goal of complete baseline separation for successful analyte quantification in chromatographic analysis, which can also provide a golden opportunity for remedying some undesired chromatographic separation experiments. More importantly, when used as afterwards remedial tools, second‐order calibration methods such as alternating trilinear decomposition (ATLD) algorithm can make the “chemical and/or physical separation” of unknown interferences unnecessary . As a result, chromatographic analysis may become considerably simple, cheap and environmentally friendly in terms of time, cost, and solvent consumption.…”