2012
DOI: 10.1186/1743-422x-9-180
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Two types of nanoparticle-based bio-barcode amplification assays to detect HIV-1 p24 antigen

Abstract: BackgroundHIV-1 p24 antigen is a major viral component of human immunodeficiency virus type 1 (HIV-1) which can be used to identify persons in the early stage of infection and transmission of HIV-1 from infected mothers to infants. The detection of p24 is usually accomplished by using an enzyme-linked immunosorbent assay (ELISA) with low detection sensitivity. Here we report the use of two bio-barcode amplification (BCA) assays combined with polymerase chain reaction (PCR) and gel electrophoresis to quantify H… Show more

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Cited by 30 publications
(25 citation statements)
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“…8 Bio-Barcode technology. 91,246 Although no case studies using "Bio-Barcode" approaches for PK sample testing support are available, pharmaceutically relevant case studies range from quantification of toxins, 247,248 food-born 249 and other human pathogens [250][251][252] over HIV 253,254 and other viruses 255,256 to human biomarkers like cytokines, 240 PSA, 257 thrombin, 258 or cytochrome c. 259,260 Subsequent to magnetoseparation and washing, immunocomplexes are dissociated and detection of DNA reporters is achieved by hybridization or PCR.…”
Section: Nanoparticle-based Methodsmentioning
confidence: 99%
“…8 Bio-Barcode technology. 91,246 Although no case studies using "Bio-Barcode" approaches for PK sample testing support are available, pharmaceutically relevant case studies range from quantification of toxins, 247,248 food-born 249 and other human pathogens [250][251][252] over HIV 253,254 and other viruses 255,256 to human biomarkers like cytokines, 240 PSA, 257 thrombin, 258 or cytochrome c. 259,260 Subsequent to magnetoseparation and washing, immunocomplexes are dissociated and detection of DNA reporters is achieved by hybridization or PCR.…”
Section: Nanoparticle-based Methodsmentioning
confidence: 99%
“…The results indicated that the bio-barcode-amplification method was superior to the conventional ELISA assay for the detection of HIV-1 p24 Gag protein in plasma with a breadth of coverage for diverse HIV-1 subtypes. Although bio-barcode based methods have further been used by many other groups with greater efficiency, the use of gold nanoparticles remain to be the common link in these processes [1618]. …”
Section: 1 Studies On Hivmentioning
confidence: 99%
“…This method, which couples an immunoassay with PCR amplification, requires subsequent PCR amplification steps in order to achieve such low sensitivity. 25 Another study used inductively coupled plasma mass spectrometry to quantify p24 antigen detection (LOD = 1.49 pg mL −1 ). 26 Improved detection sensitivity typically correlates with increased assay complexity.…”
Section: Discussionmentioning
confidence: 99%