Two-Step Mechanism of Cellular Uptake of Cationic Gold Nanoparticles Modified by (16-Mercaptohexadecyl)trimethylammonium Bromide

Žárská, Monika; Novotný, Filip; Havel, Filip; Šrámek, Michal; Bábelová, Andrea; Benada, Oldřich; Novotný, Miroslav; Saran, Hilal; Kuča, Kamil; Musílek, Kamil; Hvězdová, Zuzana; Dzijak, Rastislav; Vancurová, M; Krejčíková, Kateřina; Gabajova, Blanka; Hanzlíková, Hana; Kyjacová, Lenka; Bártek, Jiří; Proška, Jan; Hodný, Zdeněk

doi:10.1021/acs.bioconjchem.6b00491

Cited by 25 publications

(44 citation statements)

References 86 publications

(172 reference statements)

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“…As expected, the POSAB GNRs aggregated immediately after dilution in PBS and Dulbecco Modified Eagle's medium (DMEM) as was observed by the change in absorption spectra where extensive broadening and decrease of intensity of the longitudinal peak was observed. However, in contrast to MTAB GNRs, POSAB GNRs tend to form aggregates also in DMEM with 10% fetal bovine serum (10% FBS/DMEM) (Figure A,B) despite the presence of serum proteins that previously showed a nanoparticle anti‐aggregation effect . To conclude these data, the surface modification of GNRs by POSAB allowed successful replacement of the CTAB from GNRs surface, however, provided less but reasonable stable GNRs compared to highly stable alkanethiol‐(MTAB)‐covered GNRs .…”

Section: Resultsmentioning

confidence: 78%

“…Furthermore, we found that POSAB GNRs distributed subcellularly were forming aggregates in the cytoplasm close to the cell nucleus like MTAB GNRs. We performed a colocalization assay with POSAB GNRs and lysosomal marker LAMP‐1 to verify its lysosomal destination (Figure E), which was observed for MTAB GNRs . Figure F shows a quantitative analysis of MTAB GNRs and POSAB GNRs colocalization with LAMP‐1 expressed as thresholded Manders' coefficient (tM).…”

Section: Resultsmentioning

confidence: 94%

“…As mentioned above, MTAB GNRs are featured with remarkably high cellular uptake that was more than 40 times higher than at similar PEGylated GNRs . The human cervix carcinoma cell line (HeLa) that previously showed high internalization of cationic GNRs was used for cellular uptake evaluation as a model of cancer cells. At first, the size of POSAB GNRs aggregates formed after dilution in 10% FBS/DMEM was determined on the cell surface by field emission scanning electron microscopy (FE‐SEM) (Figure S7) and calculated to be 506.7 ± 265.2 nm.…”

Section: Resultsmentioning

confidence: 99%

“…Stabilization and functionalization of gold nanoparticles (GNPs) is a promising application of QAS. Cationic charge of QAS‐bearing GNPs enables their interaction with negatively charged cell membrane structures such as sulfated proteoglycans and potentiates their high cellular uptake . Cationic GNPs can serve as agents for gene transfection , drug vehicles and contrast agents .…”

Section: Introductionmentioning

confidence: 99%

“…The thiol modification enables covalent anchorage of the compound to the surface of GNRs through gold‐sulfur bond . Using MTAB GNRs as a prototype of alkyl‐QAS stabilized nanoparticles displaying high cellular uptake and low toxicity , we analyzed physico‐chemical and biological properties of newly prepared POSAB GNRs.…”

Section: Introductionmentioning

confidence: 99%

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Highly hydrophilic cationic gold nanorods stabilized by novel quaternary ammonium surfactant with negligible cytotoxicity

Salajkova

Šrámek

Maliňák

et al. 2019

Journal of Biophotonics

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The photothermal cancer therapy using cationic gold nanorods (GNRs) stabilized by quaternary ammonium salts (QAS) have a great potential to enhance conventional cancer treatment as it promises the effective eradication of cancer cells including cells resistant to radio‐ and chemo‐therapy and the stimulation of anti‐tumor immune response. However, as the cytotoxicity of the conventional alkanethiol‐QAS compounds limits their utility in medicine, here we developed GNRs modified by novel highly hydrophilic cationic surfactant composed of the quaternary ammonium group and ethylene glycol chain N,N,N‐trimethyl‐3,6,9,12,15‐pentaoxaheptadecyl‐17‐sulfanyl‐1‐ammonium bromide (POSAB) showing insignificant cytotoxicity in the free state. Surface modification of GNRs by POSAB allowed to prepare nanoparticles with good stability in water, high cellular uptake and localization in lysosomes that are a promising alternative to alkanethiol‐stabilized GNRs especially for biomedical applications.

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Section: Resultsmentioning

confidence: 78%

Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Highly hydrophilic cationic gold nanorods stabilized by novel quaternary ammonium surfactant with negligible cytotoxicity

Salajkova

Šrámek

Maliňák

et al. 2019

Journal of Biophotonics

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Plasmonic Self‐Propelled Nanomotors for Explosives Detection via Solution‐Based Surface Enhanced Raman Scattering

Novotný

Plutnar

Pumera

2019

Adv Funct Materials

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Nanomotors represent a class of artificial machines that span the chasm between molecular motors and bigger micromotors. Their importance lies in the fact that to effectively navigate and perform tasks in a biological environment without alerting the action of the immune system, the maximal size of the object has to be well below 200 nm. Fully nanosized gold/silver core/shell plasmonic nanomotors using the seeded growth wet chemical approach, which allows high scalability of synthesis of the nanomotors compared to planar substrate-based methods, is presented. Using the nanoparticle tracking analysis, the catalysis driven enhanced diffusion of the plasmonic nanomotors in the presence of low concentrations of fuel is presented and shown that the prepared nanomotors are good candidates for a solutionbased surface-enhanced Raman spectroscopy detection of picric acid, a typical explosive. In addition to the mentioned effects, ligand-exchange is performed on the nanomotors to replace the surfactant with its thiolated analog, a combination which is already proven in literature to be stealthy to the immune response of the living organism.The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/adfm.201903041. microjets, [8][9][10][11] Janus particles, [5,12,13] and particle dimers [11] in the past years, showing the possibility to construct micrometer-sized objects with physically and chemically programmed behavior for a specific environment and specific stimuli. [5,14] Such programmed behavior patterns include catalytically driven bubble-jet propulsion, [8] self-electrophoresis, [15][16][17] self-diffusiophoresis, [15,17] selfthermophoresis, [12,17] which are connected to response to external magnetic, [18] electric, [19] or electromagnetic field [20,21] and also to concentration gradients of chemical substances. [22] These micromachines are proposed to be used in biomedicinal [3] and environmental [4,6] as well as in military applications. [23] Applications of micromotors in sensing of small molecules are also developed but in much smaller extent. The sensing is usually based on (i) slowing the micromotors down by catalyst poisoning or accelerating them by providing fuel, [24,25] (ii) aggregation of the micromotors to produce higher concentrations of an analyte [26] or (iii) separation of the sensing of an analyte by collecting the micromotors be sedimentation. [26] However, direct sensing by micromotors is limited.While the area of self-propelled micromachines is well developed, the field of nanorobots, i.e., self-propelled devices of submicrometer size, is developed much less. For any micro-or nanorobots to have real-world impact, we have to use millions and billions of them. Most of the works in the field use planar substrate-based preparation technique. [17] To further push the micro/nanorobots field to real-world applications, one has to develop objects, whose synthesis is easily scalable. [27] The scalability is a property hard to fulfill for the planar-based...

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A review on nanotoxicity and nanogenotoxicity of different shapes of nanomaterials

Demir

2020

J of Applied Toxicology

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Nanomaterials (NMs) generally display fascinating physical and chemical properties that are not always present in bulk materials; therefore, any modification to their size, shape, or coating tends to cause significant changes in their chemical/physical and biological characteristics. The dramatic increase in efforts to use NMs renders the risk assessment of their toxicity highly crucial due to the possible health perils of this relatively uncharted territory. The different sizes and shapes of the nanoparticles are known to have an impact on organisms and an important place in clinical applications. The shape of nanoparticles, namely, whether they are rods, wires, or spheres, is a particularly critical parameter to affect cell uptake and site-specific drug delivery, representing a significant factor in determining the potency and magnitude of the effect. This review, therefore, intends to offer a picture of research into the toxicity of different shapes (nanorods, nanowires, and nanospheres) of NMs to in vitro and in vivo models, presenting an in-depth analysis of health risks associated with exposure to such nanostructures and benefits achieved by using certain model organisms in genotoxicity testing. Nanotoxicity experiments use various models and tests, such as cell cultures, cores, shells, and coating materials. This review article also attempts to raise awareness about practical applications of NMs in different shapes in biology, to evaluate their potential genotoxicity, and to suggest approaches to explain underlying mechanisms of their toxicity and genotoxicity depending on nanoparticle shape.

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Two-Step Mechanism of Cellular Uptake of Cationic Gold Nanoparticles Modified by (16-Mercaptohexadecyl)trimethylammonium Bromide

Cited by 25 publications

References 86 publications

Highly hydrophilic cationic gold nanorods stabilized by novel quaternary ammonium surfactant with negligible cytotoxicity

Highly hydrophilic cationic gold nanorods stabilized by novel quaternary ammonium surfactant with negligible cytotoxicity

Plasmonic Self‐Propelled Nanomotors for Explosives Detection via Solution‐Based Surface Enhanced Raman Scattering

A review on nanotoxicity and nanogenotoxicity of different shapes of nanomaterials

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