Two single mutations commonly cause qualitative change of nonspecific carboxylesterases in insects

Cui, Feng; Zhe, Liu; Wang, Hongsheng; Liu, Silu; Chang, Hai-Jing; Reeck, Gerald R.; Qiao, Chuanling; Raymond, Michel; Kang, Le

doi:10.1016/j.ibmb.2010.09.004

Cited by 64 publications

(67 citation statements)

References 31 publications

(47 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our data provide evidence for a moderate dose-dependent OP-tolerance function of wild type a-Est7, which is in line with these in vitro data. The primary sequence mutations in a-EST7 found in OP-resistant insects have been demonstrated to confer mutant ali-esterase activity also to Drosophila a-EST7 (Devonshire et al, 2003) and a broad range of distantly related CEs in vitro (Cui et al, 2011). The presented a-Est7 null mutant fly opens the possibility to test the in vivo impact of a-EST7 ali-esterase mutants on OP insecticide tolerance in Drosophila by a gene replacement strategy.…”

Section: Discussionmentioning

confidence: 98%

Functional fat body proteomics and gene targeting reveal in vivo functions of Drosophila melanogaster α-Esterase-7

Birner‐Gruenberger

Bickmeyer

Lange

et al. 2012

Insect Biochemistry and Molecular Biology

View full text Add to dashboard Cite

Carboxylesterases constitute a large enzyme family in insects, which is involved in diverse functions such as xenobiotic detoxification, lipid metabolism and reproduction. Phylogenetically, many insect carboxylesterases are represented by multienzyme clades, which are encoded by evolutionarily ancient gene clusters such as the α-Esterase cluster. Much in contrast to the vital importance attributed to carboxylesterases in general, the in vivo function of individual α-Esterase genes is largely unknown. This study employs a functional proteomics approach to identify esterolytic enzymes of the vinegar fly Drosophila melanogaster fat body. One of the fat body carboxylesterases, α-Esterase-7, was selected for mutational analysis by gene targeting to generate a deletion mutant fly. Phenotypic characterization of α-Esterase-7 null mutants and transgenic flies, which overexpress a chimeric α-Esterase-7:EGFP gene, reveals important functions of α-Esterase-7 in insecticide tolerance, lipid metabolism and lifespan control. The presented first deletion mutant of any α-Esterase in the model insect D. melanogaster generated by gene targeting not only provides experimental evidence for the endogenous functions of this gene family. It also offers an entry point for in vivo structure-function analyses of α-Esterase-7, which is of central importance for naturally occurring insecticide resistance in wild populations of various dipteran insect species.

show abstract

Section: Discussionmentioning

confidence: 98%

Functional fat body proteomics and gene targeting reveal in vivo functions of Drosophila melanogaster α-Esterase-7

Birner‐Gruenberger

Bickmeyer

Lange

et al. 2012

Insect Biochemistry and Molecular Biology

View full text Add to dashboard Cite

show abstract

“…CarE-based metabolic resistance to OPs has been observed in many insects, including cotton aphids [7]–[9]. The molecular mechanism of this resistance originates either from mutations in esterase-encoding sequences or from increased transcription of esterase genes [3], [30]. RNAi is a useful tool to probe the functions of genes.…”

Section: Discussionmentioning

confidence: 99%

“…Some mutations (G137D and W251L/S) in esterase genes that confer resistance to OPs have been reported in Musca domestica [2], [23]–[25], Lucilia cuprina [26]–[27], Cochliomyia hominivorax [28] and Culex pipiens [29]. Cui et al [30] reported that G/A151D or W271L mutations could be common mechanisms in the development of OP resistance in Dipteran species.…”

Section: Introductionmentioning

confidence: 99%

Oral Delivery Mediated RNA Interference of a Carboxylesterase Gene Results in Reduced Resistance to Organophosphorus Insecticides in the Cotton Aphid, Aphis gossypii Glover

Gong

Shang

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

BackgroundRNA interference (RNAi) is an effective tool to examine the function of individual genes. Carboxylesterases (CarE, EC 3.1.1.1) are known to play significant roles in the metabolism of xenobiotic compounds in many insect species. Previous studies in our laboratory found that CarE expression was up-regulated in Aphis gossypii (Glover) (Hemiptera: Aphididae) adults of both omethoate and malathion resistant strains, indicating the potential involvement of CarE in organophosphorus (OP) insecticide resistance. Functional analysis (RNAi) is therefore warranted to investigate the role of CarE in A. gossypii to OPs resistance.Result CarE expression in omethoate resistant individuals of Aphis gossypii was dramatically suppressed following ingestion of dsRNA-CarE. The highest knockdown efficiency (33%) was observed at 72 h after feeding when dsRNA-CarE concentration was 100 ng/µL. The CarE activities from the CarE knockdown aphids were consistent with the correspondingly significant reduction in CarE expression. The CarE activity in the individuals of control aphids was concentrated in the range of 650–900 mOD/per/min, while in the individuals of dsRNA-CarE-fed aphids, the CarE activity was concentrated in the range of 500–800 mOD/per/min. In vitro inhibition experiments also demonstrated that total CarE activity in the CarE knockdown aphids decreased significantly as compared to control aphids. Bioassay results of aphids fed dsRNA-CarE indicated that suppression of CarE expression increased susceptibility to omethoate in individuals of the resistant aphid strains.ConclusionThe results of this study not only suggest that ingestion of dsRNA through artificial diet could be exploited for functional genomic studies in cotton aphids, but also indicate that CarE can be considered as a major target of organophosphorus insecticide (OPs) resistance in A. gossypii. Further, our results suggest that the CarE would be a propitious target for OPs resistant aphid control, and insect-resistant transgenic plants may be obtained through plant RNAi-mediated silencing of insect CarE expression.

show abstract

“…The coding sequences of COEs were rich in SNPs (Supplementary Fig. 25a), which can be critical in determining COE substrate specificity and catalytic activity under xenobiotic stresses 31 . Principal-component analysis indicated that intronic regions consistently harbored all types of polymorphic variations, whereas coding sequences were frequently polymorphic for structural variations and transposable elements, which may have a pronounced effect on gene function (Fig.…”

Section: (Supplementarymentioning

confidence: 99%

A heterozygous moth genome provides insights into herbivory and detoxification

et al. 2013

View full text Add to dashboard Cite

l e t t e r sHow an insect evolves to become a successful herbivore is of profound biological and practical importance. Herbivores are often adapted to feed on a specific group of evolutionarily and biochemically related host plants 1 , but the genetic and molecular bases for adaptation to plant defense compounds remain poorly understood 2 . We report the first whole-genome sequence of a basal lepidopteran species, Plutella xylostella, which contains 18,071 protein-coding and 1,412 unique genes with an expansion of gene families associated with perception and the detoxification of plant defense compounds. A recent expansion of retrotransposons near detoxification-related genes and a wider system used in the metabolism of plant defense compounds are shown to also be involved in the development of insecticide resistance. This work shows the genetic and molecular bases for the evolutionary success of this worldwide herbivore and offers wider insights into insect adaptation to plant feeding, as well as opening avenues for more sustainable pest management.The global pest P. xylostella (Lepidoptera: Yponomeutidae) is thought to have coevolved with the crucifer plant family 3 ( Supplementary Fig. 1) and has become the most destructive pest of economically important food crops, including rapeseed, cauliflower and cabbage 4 . Recently, the total cost of damage and management worldwide was estimated at $4-5 billion per annum 5,6 . This insect is the first species to have evolved resistance to dichlorodiphenyltrichloroethane (DDT) in the 1950s 7 and to Bacillus thuringiensis (Bt) toxins in the 1990s 8 and has developed resistance to all classes of insecticide, making it increasingly difficult to control 9,10 . P. xylostella provides an exceptional system for understanding the genetic and molecular bases of how insect herbivores cope with the broad range of plant defenses and chemicals encountered in the environment (Supplementary Fig. 2).We used a P. xylostella strain (Fuzhou-S) collected from a field in Fuzhou in southeastern China (26.08 °N, 119.28 °E) for sequencing ( Supplementary Fig. 1). Whole-genome shotgun-based Illumina sequencing of single individuals (Supplementary Table 1), even after ten generations of laboratory inbreeding, resulted in a poor initial assembly (N50 = 2.4 kb, representing the size above which 50% of the total length of the sequences is included), owing to high levels of heterozygosity ( Supplementary Figs. 3 and 4 and Supplementary Table 2). Subsequently, we sequenced 100,800 fosmid clones (comprising ~10× the genome length) to a depth of 200× ( Supplementary Fig. 5 and Supplementary Tables 3-5), assembling the resulting sequence data into 1,819 scaffolds, with an N50 of 737 kb, spanning ~394 Mb of the genome sequence (version 1; Supplementary Fig. 6 and Supplementary Table 6). The assembly covered 85.5% of a set of protein-coding ESTs (Supplementary Tables 7 and 8) generated by transcriptome sequencing 11 . Alignment of a subject scaffold against a 126-kb BAC (GenBank GU058050) from an altern...

show abstract

Two single mutations commonly cause qualitative change of nonspecific carboxylesterases in insects

Cited by 64 publications

References 31 publications

Functional fat body proteomics and gene targeting reveal in vivo functions of Drosophila melanogaster α-Esterase-7

Functional fat body proteomics and gene targeting reveal in vivo functions of Drosophila melanogaster α-Esterase-7

Oral Delivery Mediated RNA Interference of a Carboxylesterase Gene Results in Reduced Resistance to Organophosphorus Insecticides in the Cotton Aphid, Aphis gossypii Glover

A heterozygous moth genome provides insights into herbivory and detoxification

Contact Info

Product

Resources

About