Two serotypes of Corynebacterium pseudotuberculosis isolated from different animal species

Barakat, Abdelhamid; Selim, Salah A.; Atef, Ashraf; Saber, Mohamed; Nafie, E. K.; El-Edeeby, A A

doi:10.20506/rst.3.1.147

Cited by 31 publications

(44 citation statements)

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“…2) having the same high titer of SH but produced half the amount produced by isolate No. (1). at the same time, strain No.…”

Section: Discussionmentioning

confidence: 99%

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Synergistic haemolytic activity and its correlation to phospholipase D productivity by Corynebacteruim pseudotuberculosis Egyptian isolates from sheep and buffaloes

Selim¹,

Mousa²,

Mohamed³

et al. 2012

Braz. J. Microbiol.

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Fourteen isolates of Corynebacteruim pseudotuberculosis of them 7 were isolated from sheep with Caseous Lymphadenitis "biotype 1" and 7 isolated from buffaloes with Oedematous Skin Disease "biotype 2". All isolates were identified by standard microbiological techniques and by polymerase chain reaction targeting, 16S rRNA and phospholipase D genes. Synergistic haemolytic titers of all isolates were assayed by plate technique. The presences of phospholipase D gene in supernatants of all isolates were performed by sodium dodecyl sulfate polyacrylamide gel electrophoresis immunoblot technique by using hyperimmune serum raised in rabbit immunized with recombinant phospholipase D gene antigen. The concentration of phospholipase D gene was assayed by scanning the bound phospholipase D gene with specific antibodies that appeared at 31.5 kDa. Results presented that there is no correlation between titer of Synergistic haemolytic activity and the actual phospholipase D genes concentration in culture supernatants. Also results presented that Synergistic haemolytic activity and phospholipase D genes produced by biotype 2 (buffalo isolates) was generally higher than those by biotype 1(sheep isolates).

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“…2) having the same high titer of SH but produced half the amount produced by isolate No. (1). at the same time, strain No.…”

Section: Discussionmentioning

confidence: 99%

“…Biotypes were determined by conventional tests as described previously (1) in addition to starch hydrolysis.…”

Section: Methodsmentioning

confidence: 99%

Synergistic haemolytic activity and its correlation to phospholipase D productivity by Corynebacteruim pseudotuberculosis Egyptian isolates from sheep and buffaloes

Selim¹,

Mousa²,

Mohamed³

et al. 2012

Braz. J. Microbiol.

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“…Despite the broad host spectrum, natural cross-species transmission of C. pseudotuberculosis between small ruminants and cattle does not appear to occur [12], although infections of cattle with both biovars have been previously reported [31].…”

Section: Introductionmentioning

confidence: 98%

The Pan-Genome of the Animal Pathogen Corynebacterium pseudotuberculosis Reveals Differences in Genome Plasticity between the Biovar ovis and equi Strains

et al. 2013

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Corynebacterium pseudotuberculosis is a facultative intracellular pathogen and the causative agent of several infectious and contagious chronic diseases, including caseous lymphadenitis, ulcerative lymphangitis, mastitis, and edematous skin disease, in a broad spectrum of hosts. In addition, Corynebacterium pseudotuberculosis infections pose a rising worldwide economic problem in ruminants. The complete genome sequences of 15 C. pseudotuberculosis strains isolated from different hosts and countries were comparatively analyzed using a pan-genomic strategy. Phylogenomic, pan-genomic, core genomic, and singleton analyses revealed close relationships among pathogenic corynebacteria, the clonal-like behavior of C. pseudotuberculosis and slow increases in the sizes of pan-genomes. According to extrapolations based on the pan-genomes, core genomes and singletons, the C. pseudotuberculosis biovar ovis shows a more clonal-like behavior than the C. pseudotuberculosis biovar equi. Most of the variable genes of the biovar ovis strains were acquired in a block through horizontal gene transfer and are highly conserved, whereas the biovar equi strains contain great variability, both intra- and inter-biovar, in the 16 detected pathogenicity islands (PAIs). With respect to the gene content of the PAIs, the most interesting finding is the high similarity of the pilus genes in the biovar ovis strains compared with the great variability of these genes in the biovar equi strains. Concluding, the polymerization of complete pilus structures in biovar ovis could be responsible for a remarkable ability of these strains to spread throughout host tissues and penetrate cells to live intracellularly, in contrast with the biovar equi, which rarely attacks visceral organs. Intracellularly, the biovar ovis strains are expected to have less contact with other organisms than the biovar equi strains, thereby explaining the significant clonal-like behavior of the biovar ovis strains.

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“…Strains that reduce nitrate are referred as biotype equi and strains that fail to do so are referred as biotype ovis. Barakat et al (1984) found two serotypes referred as type I and type II which corresponded with nitrate reductase negative and positive strains, respectively. In camels, only the serotype I (biotype ovis) has been described in the available literature (Wernery and Kaaden 2002).…”

Section: Introductionmentioning

confidence: 96%

Isolation of nitrate-reductase positive Corynebacterium pseudotuberculosis from dromedary camels

Tejedor-Junco

Lupiola

Schulz

et al. 2007

Trop Anim Health Prod

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Two serotypes of Corynebacterium pseudotuberculosis isolated from different animal species

Cited by 31 publications

References 1 publication

Synergistic haemolytic activity and its correlation to phospholipase D productivity by Corynebacteruim pseudotuberculosis Egyptian isolates from sheep and buffaloes

Synergistic haemolytic activity and its correlation to phospholipase D productivity by Corynebacteruim pseudotuberculosis Egyptian isolates from sheep and buffaloes

The Pan-Genome of the Animal Pathogen Corynebacterium pseudotuberculosis Reveals Differences in Genome Plasticity between the Biovar ovis and equi Strains

Isolation of nitrate-reductase positive Corynebacterium pseudotuberculosis from dromedary camels

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