Isolation of nitrate-reductase positive Corynebacterium pseudotuberculosis from dromedary camels

Tejedor-Junco, María Teresa; Lupiola, P.; Schulz, U; Gutiérrez, Carlos

doi:10.1007/s11250-007-9077-2

Cited by 16 publications

(12 citation statements)

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“…Isolates from the Equi biovar (nitrate positive) are known for causing Oedematous Skin Disease (OSD) in buffaloes [ 26 ]. Equi isolates have also been found in horses [ 27 , 28 ], cattle [ 22 , 29 ] and camels [ 30 ], with different manifestation in each host species. Cattle and camels are the only cross-over hosts in that both Ovis and Equi strains have been isolated from them, but each biovars present a different disease phenotype.…”

Section: Introductionmentioning

confidence: 99%

Rapidly evolving changes and gene loss associated with host switching in Corynebacterium pseudotuberculosis

et al. 2018

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Phylogenomics and genome scale positive selection analyses were performed on 29 Corynebacterium pseudotuberculosis genomes that were isolated from different hosts, including representatives of the Ovis and Equi biovars. A total of 27 genes were identified as undergoing adaptive changes. An analysis of the clades within this species and these biovars, the genes specific to each branch, and the genes responding to selective pressure show clear differences, indicating that adaptation and specialization is occurring in different clades. These changes are often correlated with the isolation host but could indicate responses to some undetermined factor in the respective niches. The fact that some of these more-rapidly evolving genes have homology to known virulence factors, antimicrobial resistance genes and drug targets shows that this type of analysis could be used to identify novel targets, and that these could be used as a way to control this pathogen.

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Section: Introductionmentioning

confidence: 99%

Rapidly evolving changes and gene loss associated with host switching in Corynebacterium pseudotuberculosis

et al. 2018

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“…Corynebacterium pseudotuberculosis is a facultative intracellular bacterium that is the causative agent of caseous lymphadenitis (CLA) in goats and sheep, abscesses and ulcerative lymphangitis in horses and oedematous skin disease (OSD) in Buffalo. It also causes sporadic infections in other species including cattle, camels, llamas, and humans [ 1 – 4 ]. C. pseudotuberculosis can be classified in two biovars, based on their ability to convert nitrate to nitrite, nitrate-positive strains are classified as biovar Equi and the nitrate negative ones as biovar Ovis [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Quadruplex PCR assay for identification of Corynebacterium pseudotuberculosis differentiating biovar Ovis and Equi

et al. 2017

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Background Corynebacterium pseudotuberculosis is classified into two biovars, nitrate-negative biovar Ovis which is the etiologic agent of caseous lymphadenitis in small ruminants and nitrate-positive biovar Equi, which causes abscesses and ulcerative lymphangitis in equines. The aim of this study was to develop a quadruplex PCR assay that would allow simultaneous detection and biovar-typing of C. pseudotuberculosis.MethodsIn the present study, genomes of C. pseudotuberculosis strains were used to identify the genes involved in the nitrate reduction pathway to improve a species identification three-primer multiplex PCR assay. The nitrate reductase gene (narG) was included in the PCR assay along with the 16S, rpoB and pld genes to enhance the diagnosis of the multiplex PCR at biovar level.ResultsA novel quadruplex PCR assay for C. pseudotuberculosis species and biovar identification was developed. The results of the quadruplex PCR of 348 strains, 346 previously well-characterized clinical isolates of C. pseudotuberculosis from different hosts (goats, sheep, horse, cattle, buffalo, llamas and humans), the vaccine strain 1002 and the type strain ATCC 19410T, were compared to the results of nitrate reductase identification by biochemical test. The McNemar’s Chi-squared test used to compare the two methods used for C. pseudotuberculosis biovar identification showed no significant difference (P = 0.75) [95% CI for odds ratio (0.16–6.14)] between the quadruplex PCR and the nitrate biochemical test. Concordant results were observed for 97.13% (338 / 348) of the tested strains and the kappa value was 0.94 [95% CI (0.90–0.98)].ConclusionsThe ability of the quadruplex assay to discriminate between C. pseudotuberculosis biovar Ovis and Equi strains enhances its usefulness in the clinical microbiology laboratory.Electronic supplementary materialThe online version of this article (10.1186/s12917-017-1210-5) contains supplementary material, which is available to authorized users.

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“…and Staphylococcus spp. may be involved in CLA of camels (Tejedor et al, 2000;Tejedor-Junco et al, 2008;Aljameel et al, 2013;Zidan et al, 2013). Infection is usually a wound contamination, but inhalation and ingestion were also reported (Wernery, 2012).…”

Section: Introductionmentioning

confidence: 99%

Caseous Lymphadenitis in Sudanese and Somalian Camels Imported for Meat Consumption in Egypt

Borham¹,

Oreiby²,

El-Gedawy³

et al. 2017

AJVS

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Key words:CLA camel clinical postmortem microbiological histopathology This study was conducted on Sudanese and Somalian imported camels at AlWarraq abattoir, Giza (Egypt), during the period from September 2014 to November 2015. A total of 850 adult one-humped camels (Camelus dromedarius) were subjected to clinical and postmortem examinations. Caseous lymphadenitis lesions were detected in 88 (10.35%) animals. Superficial form (9.76%) was significantly more prevalent than the visceral form (0.58%). Incised lesions showed cheesy greenish-whitish pus, four lesions were congested without abscess formation. Coryneform bacteria were isolated as a pure culture in 11 (30.55%) cases. Targeting Corynebacterium pseudotuberculosis serotype 1, being a common cause of caseous lymphadenitis, two isolates were identified by pld gene-specific polymerase chain reaction. Other coryneforms were tested by the API Coryne system; three Cellulomonas spp./Microbacterium spp. and one Brevibacterium spp. were identified. The APIunidentified bacteria were subjected to the Vitek 2 system which identified two isolates as Corynebacterium jeikeium and Corynebacterium urealyticum. The Vitek 2 systemunidentified isolates were classified as unidentified coryneforms. Histopathological findings revealed suppuration, necrosis, hyperplastic lymphoid follicles and sinus histocytosis of the affected lymph nodes. In contrast to small ruminants, few studies are available on caseous lymphadenitis in camel and rare of them involve the visceral form. In conclusion, caseous lymphadenitis is prevalent in Sudanese and Somali imported camels in Egypt. In camels, the disease has variable natures and visceral lesions are uncommon. Coryneform bacteria other than Corynebacterium pseudotu1berculosis are associated with the disease in camels which necessitate multiple identification steps.

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Isolation of nitrate-reductase positive Corynebacterium pseudotuberculosis from dromedary camels

Cited by 16 publications

References 9 publications

Rapidly evolving changes and gene loss associated with host switching in Corynebacterium pseudotuberculosis

Rapidly evolving changes and gene loss associated with host switching in Corynebacterium pseudotuberculosis

Quadruplex PCR assay for identification of Corynebacterium pseudotuberculosis differentiating biovar Ovis and Equi

Caseous Lymphadenitis in Sudanese and Somalian Camels Imported for Meat Consumption in Egypt

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