Two Rab5 Homologs Are Essential for the Development and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae

Yang, Chuankai; Dang, Xie; Zheng, Hua wei; Chen, Xiao feng; Lin, Xiao; Zhang, Dong mei; Abubakar, Yakubu Saddeeq; Chen, Xin; Lu, Guodong; Wang, Zonghua; Li, Guangpu; Zhou, Jie

doi:10.3389/fpls.2017.00620

Cited by 17 publications

(15 citation statements)

References 42 publications

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“…The SNARE proteins MoVam7, MoSyn8, and FgVam7 in P. oryzae and F. graminearum have been identified to participate in endocytosis and pathogenicity, respectively (Dou et al, 2011;Qi et al, 2016;Zhang et al, 2016). Deletion of the endocytosis-related gene RAB5 in P. oryzae and F. graminearum results in growth defect and complete loss of the infection to plants (Zheng et al, 2015;Yang et al, 2017). Moreover, Vps9, a Vps21 GEF, colocalizes with the early endosome marker Vps21 and plays an important role in vegetative growth, endocytosis, and pathogenicity in the rice blast fungus P. oryzae and the tomato pathogen Fol (Zhu et al, 2018;Li et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

FgVps9, a Rab5 GEF, Is Critical for DON Biosynthesis and Pathogenicity in Fusarium graminearum

Yang

Zhang

et al. 2020

Front. Microbiol.

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Rab GTPases play an important role in vesicle-mediated membrane trafficking in eukaryotes. Previous studies have demonstrated that deletion of RAB5/VPS21 reduces endocytosis and virulence of fungal phytopathogens in their host plants. However, Rab5 GTPase cycle regulators have not been characterized in Fusarium graminearum, the causal agent of Fusarium head blight (FHB) or head scab disease in cereal crops. In this study, we have identified and characterized a Rab5 guanine nucleotide exchange factor (GEF), the Vps9 homolog FgVps9, in F. graminearum. Yeast two hybrid (Y2H) assays have shown that FgVps9 specifically interacts with the guanosine diphosphate (GDP)bound (inactive) forms of FgRab51 and FgRab52, the Rab5 isoforms in F. graminearum. Deletion of FgVPS9 shows impaired fungal growth and conidiation. Pathogenicity assays indicate that deletion of FgVPS9 can significantly decrease the virulence of F. graminearum in wheat. Cytological analyses have indicated that FgVps9 colocalizes with FgRab51 and FgRab52 on early endosomes and regulates endocytosis and autophagy processes. Gene expression and cytological examination have shown that FgVps9 and FgRab51 or FgRab52 function in concert to control deoxynivalenol (DON) biosynthesis by regulating the expression of trichothecene biosynthesis-related genes and toxisome biogenesis. Taken together, FgVps9 functions as a GEF for FgRab51 and FgRab52 to regulate endocytosis, which, as a basic cellular function, has significant impact on the vegetative growth, asexual development, autophagy, DON production, and plant infection in F. graminearum.

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Section: Discussionmentioning

confidence: 99%

FgVps9, a Rab5 GEF, Is Critical for DON Biosynthesis and Pathogenicity in Fusarium graminearum

Yang

Zhang

et al. 2020

Front. Microbiol.

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“…The wild-type strain PH-1 (Cuomo et al, 2007) and mutant strains of F. graminearum generated in this study were routinely cultured on CM, SYM (1% starch, 0.6% yeast extract, 0.3% sucrose and 2% agar) (Yang et al, 2017), and 5× YEG plates at 25 C. The growth rate on CM and 5× YEG plates, conidiation in liquid CMC cultures and mating on carrot agar plates were assayed as previously described (Hou et al, 2002;Zhou et al, 2010;Cavinder et al, 2012;Cao et al, 2016). Protoplast preparation and polyethylene glycol-mediated transformation were performed as described (Wang et al, 2011).…”

Section: Strains and Culture Conditionsmentioning

confidence: 99%

FgPal1 regulates morphogenesis and pathogenesis in Fusarium graminearum

Yin

Hao

Niu

et al. 2020

Environmental Microbiology

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Ascospores are the primary inoculum in Fusarium graminearum, a causal agent of wheat head blight. In a previous study, FgPAL1 was found to be upregulated in the Fgama1 mutant and important for ascosporogenesis. However, the biological function of this well-conserved gene in filamentous ascomycetes is not clear. In this study, we characterized its functions in growth, differentiation and pathogenesis. The Fgpal1 mutant had severe growth defects and often displayed abnormal hyphal tips. It was defective in infectious growth in rachis tissues and spreading in wheat heads. The Fgpal1 mutant produced conidia with fewer septa and more nuclei per compartment than the wild type. In actively growing hyphal tips, FgPal1-GFP mainly localized to the subapical collar and septa. The FgPal1 and LifeAct partially co-localized at the subapical region in an interdependent manner. The Fgpal1 mutant was normal in meiosis with eight nuclei in developing asci but most asci were aborted. Taken together, our results showed that FgPal1 plays a role in maintaining polarized tip growth and coordination between nuclear division and cytokinesis, and it is also important for infectious growth and developments of ascospores by the free cell formation process.

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“…It was recently found that M. oryzae protein MoEnd3 is critical for development and virulence due to its role in mediating receptor endocytosis (Li et al 2017b ). Additionally, MoRab5A and MoRab5B, both of which are Rab5 homologs in M. oryzae , are critical for endocytosis and plant pathogenesis, among other things (Yang et al 2017 ). Other studies have linked MoArk1, MoAbp1, MoAct1, and MoCAP proteins (among others) to endocytosis, growth, the actin cytoskeleton, and therefore, pathogenicity (Li et al 2017a , 2019 ).…”

Section: Experimental Evidencementioning

confidence: 99%

Current views on endocytosis in filamentous fungi

Commer

Shaw

2020

Mycology

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Filamentous fungi grow by adding cell wall and membrane exclusively at the apex of tubular structures called hyphae. Growth was previously believed to occur only through exocytosis at the Spitzenkörper, an organised body of secretory macro-and microvesicles found only in growing hyphae. More recent work has indicated that an area deemed the sub-apical collar is enriched for endocytosis and is also required for hyphal growth. It is now generally believed that polarity of filamentous fungi is achieved through the balancing of the processes of endocytosis and exocytosis at these two areas. This review is an update on the current progress and understanding surrounding the occurrence of endocytosis and its spatial regulation as they pertain to growth and pathogenicity in filamentous fungi.

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Two Rab5 Homologs Are Essential for the Development and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae

Cited by 17 publications

References 42 publications

FgVps9, a Rab5 GEF, Is Critical for DON Biosynthesis and Pathogenicity in Fusarium graminearum

FgVps9, a Rab5 GEF, Is Critical for DON Biosynthesis and Pathogenicity in Fusarium graminearum

FgPal1 regulates morphogenesis and pathogenesis in Fusarium graminearum

Current views on endocytosis in filamentous fungi

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