Two polyurethanases PueA and PueB are major extracellular lipases partly secreted by the mediation of their cognate ABC exporter AprDEF in Pseudomonas protegens Pf‐5

Abstract

Significance and Impact of the Study: We investigated the additional function of two polyurethanases PueA and PueB from Pseudomonas protegens Pf-5, and demonstrated that they are major extracellular lipases that belong to the members of subfamily I.3 lipases, involved in the uptake of oil in Pf-5, of which PueA plays a more important role in extracellular lipase activity. Furthermore, their cognate ABC exporter AprDEF can partly mediate the extracellular secretion of PueA and PueB in Pf-5 and heterologous Escherichia coli BL21(DE3). Lastly, PueA and PueB can also be secreted into the extracellular environment without the help of their cognate AprDEF in Pf-5 and BL21(DE3).

Enzymes’ Power for Plastics Degradation

“…A method using this strain to remove PU coating was patented by the U.S. Ship . Two lipases PueA and PueB were also found in Pseudomonas protegens . − Deletion of the pueA gene reduced PU-clearing activity, while pueB deletion exhibited little effect. Nevertheless, removal of both genes was necessary to stop degradation of PU.…”