Use of Lipases as a Sustainable and Efficient Method for the Synthesis and Degradation of Polymers

Freije García, Fabricio; García Liñares, Guadalupe

doi:10.1007/s10924-023-03118-z

Cited by 2 publications

(1 citation statement)

References 271 publications

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“…In the PP 575 -fed sample, both genes were among the top up-regulated genes. Previous studies have reported the involvement of these two enzymes in plastic degradation. , However, their function on the nonhydrolyzable polyolefin plastics should be further investigated. Cannibalism is a common phenomenon in T.…”

Section: Resultsmentioning

confidence: 99%

Molecular-Weight-Dependent Degradation of Plastics: Deciphering Host–Microbiome Synergy Biodegradation of High-Purity Polypropylene Microplastics by Mealworms

He,

Ding,

Yang

et al. 2024

Environ. Sci. Technol.

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The biodegradation of polypropylene (PP), a highly persistent nonhydrolyzable polymer, by Tenebrio molitor has been confirmed using commercial PP microplastics (MPs) (M n 26.59 and M w 187.12 kDa). This confirmation was based on the reduction of the PP mass, change in molecular weight (MW), and a positive Δδ 13 C in the residual PP. A MW-dependent biodegradation mechanism was investigated using five high-purity PP MPs, classified into low (0.83 and 6.20 kDa), medium (50.40 and 108.0 kDa), and high (575.0 kDa) MW categories to access the impact of MW on the depolymerization pattern and associated gene expression of gut bacteria and the larval host. The larvae can depolymerize/biodegrade PP polymers with high MW although the consumption rate and weight losses increased, and survival rates declined with increasing PP MW. This pattern is similar to observations with polystyrene (PS) and polyethylene (PE), i.e., both M n and M w decreased after being fed low MW PP, while M n and/or M w increased after high MW PP was fed. The gut microbiota exhibited specific bacteria associations, such as Kluyvera sp. and Pediococcus sp. for high MW PP degradation, Acinetobacter sp. for medium MW PP, and Bacillus sp. alongside three other bacteria for low MW PP metabolism. In the host transcriptome, digestive enzymes and plastic degradation-related bacterial enzymes were up-regulated after feeding on PP depending on different MWs. The T. molitor host exhibited both defensive function and degradation capability during the biodegradation of plastics, with high MW PP showing a relatively negative impact on the larvae.

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Section: Resultsmentioning

confidence: 99%

Molecular-Weight-Dependent Degradation of Plastics: Deciphering Host–Microbiome Synergy Biodegradation of High-Purity Polypropylene Microplastics by Mealworms

He,

Ding,

Yang

et al. 2024

Environ. Sci. Technol.

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Enzymatic Degradation Behavior of Self-Degradable Lipase-Embedded Aliphatic and Aromatic Polyesters and Their Blends

Peñas,

Beloqui,

Martínez de Ilarduya

et al. 2024

Biomacromolecules

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Over the past decade, the preparation of novel materials by enzyme-embedding into biopolyesters has been proposed as a straightforward method to produce self-degrading polymers. This paper reports the preparation and enzymatic degradation of extruded self-degradable films of three different biopolyesters: poly(lactic acid) (PLA), poly(butylene adipate-coterephthalate) (PBAT), and poly(butylene succinate) (PBS), as well as three binary/ternary blends. Candida antarctica lipase B (CalB) has been employed for the enzyme-embedding procedure, and to the best of our knowledge, the use of this approach in biopolyester blends has not been reported before. The three homopolymers exhibited differentiated degradation and suggested a preferential attack of CalB on PBS films over PBAT and PLA. Moreover, the self-degradable films obtained from the blends showed slow degradation, probably due to the higher content in PLA and PBAT. These observations pave the way for exploring enzymes capable of degrading all blend components or an enzymatic mixture for blend degradation.

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Use of Lipases as a Sustainable and Efficient Method for the Synthesis and Degradation of Polymers

Cited by 2 publications

References 271 publications

Molecular-Weight-Dependent Degradation of Plastics: Deciphering Host–Microbiome Synergy Biodegradation of High-Purity Polypropylene Microplastics by Mealworms

Molecular-Weight-Dependent Degradation of Plastics: Deciphering Host–Microbiome Synergy Biodegradation of High-Purity Polypropylene Microplastics by Mealworms

Enzymatic Degradation Behavior of Self-Degradable Lipase-Embedded Aliphatic and Aromatic Polyesters and Their Blends

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