Two-photon lithography and microscopy of 3D hydrogel scaffolds for neuronal cell growth

Accardo, Angelo; Blatché, Marie-Charline; Courson, Rémi; Loubinoux, Isabelle; Vieu, Christophe; Malaquin, Laurent

doi:10.1088/2057-1976/aaab93

Cited by 77 publications

(60 citation statements)

References 47 publications

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“…Spagnolo et al have also fabricated 3D micro‐cages using TPL to disseminate between non‐tumorigenic and tumorigenic human breast cells based on their metastatic potential and the invasiveness of the cell lines at various pore sizes . Accardo et al utilized TPL to synthesize woodpile‐like poly (ethylene glycol) diacrylate (PEGDA) architectures and colonize it with neuro2A cells to form neuritic extensions or interconnections . Highly compliant tetrakaidekahedral nanolattices with tunable stiffness made of a UV curable polymer (IP‐Dip) coated with Ti, W, and TiO 2 were fabricated by Maggi et al These nanolattices were seeded with osteoblast‐like cells (SAOS‐2) and their mechanosensitive responses were investigated based on its mineral secretions, intracellular f‐actin and vinculin concentrations after cell culturing.…”

Section: Typical Examples Of Mechanical Metamaterialsmentioning

confidence: 99%

Mechanical Metamaterials and Their Engineering Applications

et al. 2019

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In the past decade, mechanical metamaterials have garnered increasing attention owing to its novel design principles which combine the concept of hierarchical architecture with material size effects at micro/nanoscale. This strategy is demonstrated to exhibit superior mechanical performance that allows us to colonize unexplored regions in the material property space, including ultrahigh strength-to-density ratios, extraordinary resilience, and energy absorption capabilities with brittle constituents. In the recent years, metamaterials with unprecedented mechanical behaviors such as negative Poisson's ratio, twisting under uniaxial forces, and negative thermal expansion are also realized. This paves a new pathway for a wide variety of multifunctional applications, for example, in energy storage, biomedical, acoustics, photonics, and thermal management. Herein, the fundamental scientific theories behind this class of novel metamaterials, along with their fabrication techniques and potential engineering applications beyond mechanics are reviewed. Explored examples include the recent progresses for both mechanical and functional applications. Finally, the current challenges and future developments in this emerging field is discussed as well.

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Section: Typical Examples Of Mechanical Metamaterialsmentioning

confidence: 99%

Mechanical Metamaterials and Their Engineering Applications

et al. 2019

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“…Whereas most of the experiments so far have been performed with commonly used cell lines, 3D scaffolds also have a high potential for the controlled culture of neurons and stem cells. First steps in this direction have been performed by the groups of Vieu and Malaquin, which demonstrated the biocompatibility of 3D scaffolds for neuroblastoma cells . Concerning stem cells, Raimondi and coworkers improved the manufacturing of synthetic 3D niche structures for culturing various types of stem cells, including mesenchymal stem cells .…”

Section: Direct Laser Writing For Cell Culturementioning

confidence: 99%

3D Scaffolds to Study Basic Cell Biology

et al. 2019

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Mimicking the properties of the extracellular matrix is crucial for developing in vitro models of the physiological microenvironment of living cells. Among other techniques, 3D direct laser writing (DLW) has emerged as a promising technology for realizing tailored 3D scaffolds for cell biology studies. Here, results based on DLW addressing basic biological issues, e.g., cell‐force measurements and selective 3D cell spreading on functionalized structures are reviewed. Continuous future progress in DLW materials engineering and innovative approaches for scaffold fabrication will enable further applications of DLW in applied biomedical research and tissue engineering.

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“…Additionally, conceptually any resin can be used, so long as it has sufficient absorption of the wavelength of the laser used to induce TPAP, and a high enough proportion of photoacid in the matrix. This allows researchers to create custom resins with the properties that they require, a feature that has already been exploited by researchers creating cell-scaffolds from hydrogels [110]. While in principle two-photon absorption polymerisation simply requires a high-speed pulsed laser, the invention of the Nanoscribe, a commercial solution developed by researchers at Karlsruhe Institute of Technology, has led to more user-friendly TPAP-based 3D printing.…”

Section: Optical Microrobots: Potential Tools For Biological Studiesmentioning

confidence: 99%

Optical Micromachines for Biological Studies

2020

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Optical tweezers have been used for biological studies since shortly after their inception. However, over the years research has suggested that the intense laser light used to create optical traps may damage the specimens being studied. This review aims to provide a brief overview of optical tweezers and the possible mechanisms for damage, and more importantly examines the role of optical micromachines as tools for biological studies. This review covers the achievements to date in the field of optical micromachines: improvements in the ability to produce micromachines, including multi-body microrobots; and design considerations for both optical microrobots and the optical trapping set-up used for controlling them are all discussed. The review focuses especially on the role of micromachines in biological research, and explores some of the potential that the technology has in this area.

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Two-photon lithography and microscopy of 3D hydrogel scaffolds for neuronal cell growth

Cited by 77 publications

References 47 publications

Mechanical Metamaterials and Their Engineering Applications

Mechanical Metamaterials and Their Engineering Applications

3D Scaffolds to Study Basic Cell Biology

Optical Micromachines for Biological Studies

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