3D Scaffolds to Study Basic Cell Biology

Hippler, Marc; Lemma, Enrico Domenico; Bertels, Sarah; Blasco, Eva; Barner‐Kowollik, Christopher; Wegener, Martin; Bastmeyer, Martin

doi:10.1002/adma.201808110

Cited by 111 publications

(98 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the biomedical field, TPA‐based DLW has drawn large attention to create patterned substrates and structures for cell–substrate interaction and mechanobiological studies . Polymeric surfaces textured with submicrometric linear ridges through this technique have been employed to perform cell–substrate interaction studies demonstrating improved axonal growth and guidance along the linear features as well as promotion of the differentiation of stem cells toward neurons .…”

Section: Structuring Techniques For Light‐sensitive Polymersmentioning

confidence: 99%

Light to Shape the Future: From Photolithography to 4D Printing

Barrio

Sánchez‐Somolinos

2019

Advanced Optical Materials

185

147

View full text Add to dashboard Cite

producing increasingly complex patterned films and miniaturized devices, which are required in modern information and communication technologies. [1] In addition to semiconductor device fabrication, a host of applications for patterned thin films and structured polymeric systems have been identified over the last two decades in areas including optics, energy harvesting, biotechnology, and medicine. [2] Our ability to structure polymeric materials has grown tremendously and the palette of materials and techniques to achieve a quick and reliable production of patterns with increasing resolution is quite extensive. This boom has been largely sustained by the development of specific photonic technologies, such as advanced light sources or spatial light modulators, photosensitive materials, and, more importantly, our growing knowledge of the interaction between light and matter. Indeed, the ability to control a chemical reaction with light in a remote fashion is a powerful concept. Light irradiation can initiate a photochemical process that otherwise would not occur at ambient conditions. This property lies at the origin of the temporal control afforded by simply turning the light source on and off. Moreover, patterned illumination or the use of focused light beams enables control of not only when, but also where a photoinduced transformation occurs. The photochemical processes occurring in many polymers and resists can be effectively manipulated by fine-tuning the intensity and frequency of light. However, these are just two of the primary properties of light, and there are specific photoresponsive systems that enable the generation of ordered structures by adjusting the polarization of light, in addition to intensity and frequency.In this review, an overview of photochemical reactions, photosensitive polymers, and light-based structuring techniques is provided. As this field is too broad to be covered in depth in one single article, some recent examples and applications, as well as emerging opportunities and trends, are highlighted throughout the discussion. Section 2 is focused on light-induced reactions and photosensitive materials. The fundamentals of photochemical reactions and their implementation in polymer science have been overviewed in many excellent books and reviews. [3] In Section 2, rather than being comprehensive, emphasis is given to those photochemical processes lying at the basis of those structuring techniques that are later discussed in Section 3. Some of these techniques, including conventional mask lithographies, interference lithographies, direct laser writing (DLW) with one-or two-photon processes, or stereolithographic techniques, make use of light as a structuring tool.There is an additional group of techniques where light is employed to set a structure created beforehand through a Over the last few decades, the demand of polymeric structures with welldefined features of different size, dimension, and functionality has increased from various application areas, including microelectronics, ...

show abstract

Section: Structuring Techniques For Light‐sensitive Polymersmentioning

confidence: 99%

Light to Shape the Future: From Photolithography to 4D Printing

Barrio

Sánchez‐Somolinos

2019

Advanced Optical Materials

185

147

View full text Add to dashboard Cite

show abstract

“…In addition to their use as support structures,c leavable photoresists open new avenues of research in cell biology, where laser-printed structures can be used as microscaffolds for cell positioning. [23] In this application, the locally controlled, or complete cleavage of cell microscaffolds has the potential to provide insights into the response of cells to environmental changes as well as the mechanisms underlying adaptive processes. [24] An alternative future application for cleavable photoresists is microfluidics.Itispredicted that 3D printing will replace most current manufacturing processes in academia for microfluidic devices,i ncluding moulding of poly(dimethylsiloxane) and plastic, as alignment problems occur with multiple layers and microchannels cannot be made with arbitrary aspect ratios.…”

Section: Applications Of Cleavable Microstructuresmentioning

confidence: 99%

It's in the Fine Print: Erasable Three‐Dimensional Laser‐Printed Micro‐ and Nanostructures

et al. 2020

Self Cite

View full text Add to dashboard Cite

3D printing, on all scales, is currently a vibrant topic in scientific and industrial research as it has enormous potential to radically change manufacturing. Owing to the inherent nature of the manufacturing process, 3D printed structures may require additional material to structurally support complex features. Such support material must be removed after printing—sometimes termed subtractive manufacturing—without adversely affecting the remaining structure. An elegant solution is the use of photoresists containing labile bonds that allow for controlled cleavage with specific triggers. Herein, we explore state‐of‐the‐art cleavable photoresists for 3D direct laser writing, as well as their potential to combine additive and subtractive manufacturing in a hybrid technology. We discuss photoresist design, feature resolution, cleavage properties, and current limitations of selected examples. Furthermore, we share our perspective on possible labile bonds, and their corresponding cleavage trigger, which we believe will have a critical impact on future applications and expand the toolbox of available cleavable photoresists.

show abstract

“…Abgesehen von der Verwendung als Stützmaterial erçffnen spaltbare Photolacke neue Forschungsgebiete in der Zellbiologie,w ol asergedruckte Strukturen als Mikrogerüst zur Zellposititionierung verwendet werden kçnnen. [23] In dieser Anwendung hat die lokal gesteuerte oder vollständige Degradierung von Zellgerüsten großes Potenzial, um Einblicke in die Zellmigration auf Umwelteinflüsse und die zugrunde liegenden Mechanismen zu geben. [24] Eine alternative Anwendung fürs paltbare Photolacke ist die Verwendung in der Mikrofluidik.…”

Section: Anwendungen Spaltbarer Mikrostrukturenunclassified

Es ist im Kleingedruckten: Löschbare dreidimensionale lasergedruckte Mikro‐ und Nanostrukturen

et al. 2020

Self Cite

View full text Add to dashboard Cite

Der 3D‐Druck in allen Dimensionen ist in der Wissenschaft‐ und Industrieforschung derzeit ein dynamisches Thema, da er ein enormes Potenzial hat, die Fertigungsindustrie radikal zu verändern. Wegen der dem Fertigungsprozess innewohnenden Besonderheit erfordern 3D‐Druckstrukturen häufig zusätzliches Trägermaterial, um komplexe Merkmale strukturell zu unterstützen. Dieses Trägermaterial muss nach dem Druck – manchmal auch subtraktive Fertigung genannt – entfernt werden, ohne die verbleibende Struktur zu beeinträchtigen. Eine elegante Lösung für dieses Problem ist der Einsatz von Photolacken mit labilen Bindungen, die eine kontrollierte Spaltung mit spezifischen Auslösern nach dem Druck ermöglichen. In diesem Kurzaufsatz diskutieren wir Design, Auflösung, Spaltungseigenschaften und aktuelle Einschränkungen ausgewählter Beispiele. Darüber hinaus teilen wir unsere Sichtweise auf mögliche labile Bindungen und den entsprechenden Spaltauslöser mit, von denen wir glauben, dass sie vielversprechendes Potenzial für zukünftige Anwendungen haben und das Repertoire der verfügbaren spaltbaren Photolacke sinnvoll erweitern werden.

show abstract

3D Scaffolds to Study Basic Cell Biology

Cited by 111 publications

References 40 publications

Light to Shape the Future: From Photolithography to 4D Printing

Light to Shape the Future: From Photolithography to 4D Printing

It's in the Fine Print: Erasable Three‐Dimensional Laser‐Printed Micro‐ and Nanostructures

Es ist im Kleingedruckten: Löschbare dreidimensionale lasergedruckte Mikro‐ und Nanostrukturen

Contact Info

Product

Resources

About