Two-Photon Fluorescent Probes for Metal Ions in Live Tissues

Sarkar, Avik; Kang, Dong Eun; Kim, Hwan Myung; Cho, Bong Rae

doi:10.1021/ic402475f

Cited by 71 publications

(43 citation statements)

References 73 publications

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“…It is a delight to note significant progress in this direction by Kim and colleagues. [121][122][123] It is our hope that this review will provide added impetus to research on fluorescence PET sensors/switches. It is clear that the examples published very recently which caught our attention, and which formed the bulk of this review, cover a broad range of targets.…”

Section: Conclusion and Perspectives For Future Researchmentioning

confidence: 99%

Current developments in fluorescent PET (photoinduced electron transfer) sensors and switches

2015

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Following a brief introduction to the principle of fluorescent PET (photoinduced electron transfer) sensors and switches, the outputs of laboratories in various countries from the past year or two are categorized and critically discussed. Emphasis is placed on the molecular design and the experimental outcomes in terms of target-induced fluorescence enhancements and input/output wavelengths. The handling of single targets takes up a major fraction of the review, but the extension to multiple targets is also illustrated. Conceptually new channels of investigation are opened up by the latter approach, e.g.'lab-on-a-molecule' systems and molecular keypad locks. The growing trends of theoretically-fortified design and intracellular application are pointed out.

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Section: Conclusion and Perspectives For Future Researchmentioning

confidence: 99%

Current developments in fluorescent PET (photoinduced electron transfer) sensors and switches

2015

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“…350–550 nm) that limits their application in subcellular organelles and deep-tissue, owing to the shallow penetration depth (less than 80 μm) as well as to photo-bleaching, photo-damage, and cellular auto fluorescence (Sensi et al, 2003; Que et al, 2008; Tomat et al, 2010; McRae et al, 2009; Meng et al, 2006; Zhou et al, 2010). Recently, Two-photon fluorescence (TPF) probes, which can be excited by two-photon absorption in the NIR wavelength, provided an opportunity to overcome the problems originated from the single-photon fluorescence technology (Denk et al, 1990; Yao and Belfield, 2012; Sarkar et al, 2013; Kim et al, 2014; Kim and Cho, 2015; Meng et al, 2012; Park et al, 2012; Sarkar et al, 2014; Jing et al, 2012; Zhang et al, 2014; Wang et al, 2014; Masanta et al, 2011; Zhang et al, 2013; Zhou et al, 2014; Yin et al, 2015). However, most of the reported two-photon fluorescent probes Zn 2+ are “turn-on” ones, using enhancement of the fluorescence intensity at only one wavelength as the detection signal.…”

Section: Introductionmentioning

confidence: 99%

A mitochondria-targeted ratiometric two-photon fluorescent probe for biological zinc ions detection

Ning

Jiang

et al. 2016

Biosensors and Bioelectronics

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A mitochondria-targeted ratiometric two-photon fluorescent probe (Mito-MPVQ) for biological zinc ions detection was developed based on quinolone platform. Mito-MPVQ showed large red shifts (68nm) and selective ratiometric signal upon Zn2+ binding. The ratio of emission intensity (I488 nm/I420 nm) increases dramatically from 0.45 to 3.79 (ca. 8-fold). NMR titration and theoretical calculation confirmed the binding of Mito-MPVQ and Zn2+. Mito-MPVQ also exhibited large two-photon absorption cross sections (150GM) at nearly 720 nm and insensitivity to pH within the biologically relevant pH range. Cell imaging indicated that Mito-MPVQ could efficiently located in mitochondria and monitor mitochondrial Zn2+ under two-photon excitation with low cytotoxicity.

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“…The penetration depth can be increased using near-infrared (NIR) excitation, which either requires an NIR-absorbing fluorophore, 295–297 or a light source capable of two-photon excitation of a UV/VIS absorbing fluorophore. 298,299 …”

Section: Fluorescence Microscopic Imaging Of Intracellular Zn(ii) mentioning

confidence: 99%

Zn(ii)-coordination modulated ligand photophysical processes – the development of fluorescent indicators for imaging biological Zn(ii) ions

et al. 2014

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Molecular photophysics and metal coordination chemistry are the two fundamental pillars that support the development of fluorescent cation indicators. In this article, we describe how Zn(II)-coordination alters various ligand-centered photophysical processes that are pertinent to developing Zn(II) indicators. The main aim is to show how small organic Zn(II) indicators work under the constraints of specific requirements, including Zn(II) detection range, photophysical requirements such as excitation energy and emission color, temporal and spatial resolutions in a heterogeneous intracellular environment, and fluorescence response selectivity between similar cations such as Zn(II) and Cd(II). In the last section, the biological questions that fluorescent Zn(II) indicators help to answer are described, which have been motivating and challenging this field of research.

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Two-Photon Fluorescent Probes for Metal Ions in Live Tissues

Cited by 71 publications

References 73 publications

Current developments in fluorescent PET (photoinduced electron transfer) sensors and switches

Current developments in fluorescent PET (photoinduced electron transfer) sensors and switches

A mitochondria-targeted ratiometric two-photon fluorescent probe for biological zinc ions detection

Zn(ii)-coordination modulated ligand photophysical processes – the development of fluorescent indicators for imaging biological Zn(ii) ions

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