Two-photon excitation spectrum of perylene in solution

Yu, Jeong-A; Nocera, Daniel G.; Leroi, G. E.

doi:10.1016/0009-2614(90)85075-n

Cited by 14 publications

(16 citation statements)

References 17 publications

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“…two-photon lineshapes are completely different, indicating that the two different excitation modes access different states. A similar two-photon absorption line shape has been measured for PER, 46,47 and other workers have shown that PER derivatives have different one-and two-photon absorption spectra, 48,49 as expected for this class of highly symmetric molecules. In PP, the one-and two-photon spectra both exhibit similar vibronic progressions with spacings of ∼20 nm (corresponding to a 1000−1200 cm −1 vibrational mode characteristic of PAH's).…”

Section: ■ Experimental Sectionsupporting

confidence: 78%

Assessing the Potential of Peropyrene as a Singlet Fission Material: Photophysical Properties in Solution and the Solid State

et al. 2013

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The photophysical behavior of the polycyclic aromatic hydrocarbon peropyrene is studied both in dilute solution and in the solid state, with the goal of evaluating this molecule as a singlet fission (SF) material. In solution, the fluorescence quantum yield is consistently in the range 0.90−0.95, while the fluorescence lifetime changes from 3.2 to 5.5 ns. Analysis of the solvent dependence of the radiative rate provides evidence that the bright 1 B u singlet state mixes with a second, optically dark state. The presence of a dark state slightly above the 1 B u state in energy is confirmed using two-photon fluorescence excitation spectroscopy. The crystal structure of solid peropyrene consists of a herringbone arrangement of π-stacked molecular pairs, similar to the αpolymorph of perylene. There are two emitting species, centered at approximately 550 and 650 nm, both of which are formed within the 15 ps time resolution of the experiment, and which relax independently via biexponential decays. We find no evidence for rapid SF in the peropyrene crystals, most likely due to the large shift of the singlet state to lower energy where it no longer fulfills the energy condition for SF. These results demonstrate how both energetics and crystal packing influence the ability of a molecule to function as a SF material.

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Section: ■ Experimental Sectionsupporting

confidence: 78%

Assessing the Potential of Peropyrene as a Singlet Fission Material: Photophysical Properties in Solution and the Solid State

et al. 2013

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“…Normalizing with respect to S XX yields ,− where S = S YY / S XX and D = S XY / S XX . Values of Ω range from 1 / 4 to 3 / 2 depending on the symmetry of the participating electronic and vibrational states ,− and local solvation effects . The fluorescence anisotropies immediately after excitation by linearly and circularly polarized 2PA, R lin (0) and R circ (0) respectively, are also determined by the components of according to where θ M is the angle made by the emission transition dipole with respect to the x -axis of the molecular frame.…”

Section: Introductionmentioning

confidence: 99%

Polarized Two-Photon Absorption and Heterogeneous Fluorescence Dynamics in NAD(P)H

et al. 2019

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Two-photon absorption (2PA) finds widespread application in biological systems, which frequently exhibit heterogeneous fluorescence decay dynamics corresponding to multiple species or environments. By combining polarized 2PA with time-resolved fluorescence intensity and anisotropy decay measurements, we show how the two-photon transition tensors for the components of a heterogeneous population can be separately determined, allowing structural differences between the two fluorescent states of the redox cofactor NAD(P)H to be identified. The results support the view that the two states correspond to alternate configurations of the nicotinamide ring, rather than folded and extended conformations of the entire molecule.

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“…Two‐photon excitation of fluorophores can be achieved by focusing the beam of a pulsed NIR laser. For two‐photon excitation, the pulse width can be as long as several nanoseconds ( Yu et al ., 1990 ) and even continuous wave light can be used ( Hänninen et al ., 1994 ). However, femtosecond excitation pulses are commonly used in order to minimize the thermal load on the sample.…”

Section: Introductionmentioning

confidence: 99%

Time‐gated fluorescence lifetime imaging and microvolume spectroscopy using two‐photon excitation

Sytsma

Vroom

Grauw

et al. 1998

Journal of Microscopy

105

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SummaryA scanning microscope utilizing two-photon excitation in combination with fluorescence lifetime contrast is presented. The microscope makes use of a tunable femtosecond titanium:sapphire laser enabling the two-photon excitation of a broad range of fluorescent molecules, including UV probes. Importantly, the penetration depth of the twophoton exciting (infra)red light is substantially greater than for the corresponding single-photon wavelength while photobleaching is significantly reduced.The time structure of the Ti:Sa laser can be employed in a straightforward way for the realization of fluorescence lifetime imaging. The fluorescence lifetime is sensitive to the local environment of the fluorescent molecule. This behaviour can be used for example to quantify concentrations of ions, such as pH and Ca 2þ , or pO 2 and pCO 2 . In the set-up presented here the fluorescence lifetime imaging is accomplished by time-gated single photon counting.The performance and optical properties of the microscope are investigated by a number of test measurements on fluorescent test beads. Point-spread functions calculated from measurements on 230-nm beads using an iterative restoration procedure compare well with theoretical expectations.Lifetime imaging experiments on a test target containing two different types of test bead in a fluorescent buffer all with different lifetimes (2·15 ns, 2·56 ns and 3·34 ns) show excellent quantitative agreement with reference values obtained from time correlated single photon counting measurements. Moreover, the standard deviation in the results can be wholly ascribed to the photon statistics.Measurements of acridine orange stained biofilms are presented as an example of the potential of two-photon excitation combined with fluorescence lifetime contrast. Fluorescence lifetime and intensity images were recorded over the whole sample depth of 100 mm. Fluorescence intensity imaging is seriously hampered by the rapid decrease of the fluorescence signal as a function of the depth into the sample. Fluorescence lifetime imaging on the other hand is not affected by the decrease of the fluorescence intensity.

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Two-photon excitation spectrum of perylene in solution

Cited by 14 publications

References 17 publications

Assessing the Potential of Peropyrene as a Singlet Fission Material: Photophysical Properties in Solution and the Solid State

Assessing the Potential of Peropyrene as a Singlet Fission Material: Photophysical Properties in Solution and the Solid State

Polarized Two-Photon Absorption and Heterogeneous Fluorescence Dynamics in NAD(P)H

Time‐gated fluorescence lifetime imaging and microvolume spectroscopy using two‐photon excitation

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