Two-photon AIE bio-probe with large Stokes shift for specific imaging of lipid droplets

Abstract

A novel AIEgen with prominent two-photon excitation was rationally developed for specific lipid-droplet imaging in cells and tissues.

An Intramolecular Charge Transfer and Aggregation Induced Emission Enhancement Fluorescent Probe Based on 2‐Phenyl‐1,2,3‐triazole for Highly Selective and Sensitive Detection of Homocysteine and Its Application in Living Cells

“…This can be attributed to that the aggregates of APTC would be formed due to its poor solubility in high water fraction solution, which leads to the limitations of intramolecular motion and activates AIEE effects. When the water fraction increased from 70% to 99%, the fluorescence intensity decreased again, which may be due to the autofluorescence absorption phenomenon of the numerous aggregates, and the aggregation morphology changed (Figure c), this phenomenon is consistent with recent reports …”

Luminescent Molecular Octopuses with a Polyhedral Oligomeric Silsesquioxane (POSS) Core and Iridium(III) Polypyridine Arms: Synthesis, Aggregation Induced Emission, Cellular Uptake, and Bioimaging Studies

“…The possible AIE behavior of complexes 1 a – 3 a was studied by measuring the emission spectra of these complexes in a CH 3 CN/H 2 O mixtures with various volumetric water fractions f w at 298 K . As shown in Figure , both complexes 1 a and 2 a showed very weak emission in neat CH 3 CN ( f w =0).…”

Simple Aggregation‐Induced Emission‐Based Multifunctional Fluorescent Dots for Cancer Therapy In Vitro

“…TPAPP‐CHO was characterized by a prominent solvatochromic effect, where its FL emission was blue shifted and FL intensity was dramatically increased as decreasing the polarity of the solvent (Figure c). The photophysical properties of TPAPP‐CHO, such as showing various colours in different solvents (Figure S7), are highly dependent on the solvent polarity, which is ascribed to the twisted intramolecular charge transfer (TICT) effect from the electron‐donating TPA unit to the electron‐accepting aldehyde functionality . Meanwhile, the AIE behaviors of TPAPP‐CHO were evaluated by fluorescence assay.…”

“…As shown in Figure a, the good merging of the green and red fluorescence was clearly appeared at 4 h, and the correlation coefficient for the two images was calculated to be 97.1 %, which demonstrated the colocalization of TPAPP‐CHO NPs and lyso‐tracker. Although there are various types of fluorescent probes with AIE property developed for biological imaging, TPAPP‐CHO displayed unique superior performance on biological imaging, showing that the emission wavelength of TPAPP‐CHO NPs has significant differences in cell from in vitro . Under the channel of λ ex =488 nm/λ em =530 nm (filtering fluorescence after 530 nm), TPAPP‐CHO NPs showed strong green fluorescence, along with many shinning spots in the cells (Figures a and S4), while a weak red fluoresence (λ em =650 nm) was observed in vitro.…”