Two new species of the genus Micromonospora: Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. isolated from the insects

Abstract

Two novel actinobacteria, strains NEAU-CX1(T) and NEAU-JC6(T), were isolated from nymphs of stinkbug (Palomena viridissima Poda) and a beetle (Harpalus sinicus Hope), respectively, collected from Harbin, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The phylogenetic analysis based on 16S rRNA gene sequence of strain NEAU-CX1(T) showed it to be most closely related to Micromonospora coxensis JCM 13248(T) (99.3 % sequence similarity), Micromonospora purpureochromogenes DSM 43821(T) (99.1 %) and Micromonospora halophytica JCM 3125(T) (98.6 %), and that of strain NEAU-JC6(T) to Micromonospora haikouensis DSM 45626(T) (99.3 %), Micromonospora carbonacea JCM 3139(T) (99.1 %) and Micromonospora krabiensis JCM 12869(T) (99.1 %). The phylogenetic analysis based on gyrB gene sequence of strain NEAU-CX1(T) showed it to be most closely related to M. purpureochromogenes DSM 43821(T) (98.0 % sequence similarity), and that of strain NEAU-JC6(T) to M. haikouensis DSM 45626(T) (99.0 %) and M. carbonacea JCM 3139(T) (98.0 %). A combination of DNA-DNA hybridization results and cultural and physiological properties indicated that the two strains can be distinguished from their closest phylogenetic relatives. Thus, strains NEAU-CX1(T) and NEAU-JC6(T) represent two novel species of the genus Micromonospora, for which the names Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. are proposed. The type strains are NEAU-CX1(T) (=CGMCC 4.7175(T) = JCM 30056(T)) and NEAU-JC6(T) (=CGMCC 4.7173(T) = JCM 30055(T)).

Genome-based classification of micromonosporae with a focus on their biotechnological and ecological potential

“…At the time of writing the genus encompasses 79 species with validly published names ( http://www.bacterio.net/micromonospora.html ) 50 , the majority of which have been described using polyphasic methods 44 , 51 though there is evidence that the taxon remains underspeciated 52 , 53 . Initially, micromonosporae were associated with soil, freshwater and marine habitats 44 but novel strains have been isolated from animal 54 – 56 and plant tissues 57 – 64 , as well as from limestone 65 , Antarctic sandstone 66 and from a nickel mining site 67 . Micromonosporae form a tight cluster within the Micromonosporaceae 16S rRNA gene tree 44 , 51 though 16S rRNA gene sequences are not sufficiently divergent to distinguish between closely related strains thereby drawing upon the need for associated DNA-DNA relatedness studies 68 , 69 .…”

Recent progress on the development of antibiotics from the genus Micromonospora

“…This remained the only known strain among the actinomycetes bearing the generic name Micromonospora until Jensen in 1930 described a large number of soil micro-organisms which corresponded with Ørskov's description [19]. At the time of writing, the genus consisted of 61 validly named species (http://www.bacterio.net) [20], in addition to the recently described species, like: M. spongicola [21], M. jinlongensis [22], M. zeae [23], M. maoerensis [24], M. endophytica [25], M. palomenae [26], M. harpali [26], M. oryzae [27], M. vulcania [28], M. fluostatini [29], M. nickelidurans [30], M.…”

Micromonospora sediminis sp. nov., isolated from mangrove sediment

“…In 2015, seven species of the genus Micromonospora, 'M. endophytica ' (Thanaboripat et al, 2015), M. oryzae (Kittiwongwattana et al, 2015), M. costi (Thawai, 2015), M. palomenae, M. harpali (Fang et al, 2015), 'M. lycii ' (Zhang et al, 2015) and M. fluostatini (Phongsopitanun et al, 2015), were described.…”