Two new species of the genus Candida in the Zygoascus clade, Candida lundiana sp. nov. and Candida suthepensis sp. nov., isolated from raw honey in Thailand

Abstract

During a survey of yeasts associated with raw honey collected in Thailand, two strains of the Zygoascus clade were isolated from the Asian cavity-nesting honeybee Apis cerana and the stingless bee Homotrigona fimbriata. Phylogeny based on 26S rDNA D1/D2 sequences placed these yeasts as members of a clade including Candida bituminiphila, Candida patagonica and Candida polysorbophila. The strains of the two novel species, CBS 12271(T) and CBS 12270(T), respectively, could be unquestionably distinguished from their relatives by rDNA sequences and other taxonomic characteristics. Therefore, the novel anamorphic species, Candida lundiana sp. nov. (type strain CBS 12271(T) = JCM 16823(T)) and Candida suthepensis sp. nov. (type strain CBS 12270(T) = JCM 16822(T)) are described.

Antiviral Activity of Beebread, Bee-Collected Pollen and Artificially Fermented Pollen against Influenza A Virus

“…Yeasts are present in flower pollen, BCP and BB and actively take part in the fermentation of BCP [28,31]. Osmotolerant yeasts such as Zygosaccharomyces siamensis are able to survive in an environment with low water activity, such as honey and BB [32]. Recently, Poyraz et al artificially fermented BCP using a starter culture, combining strains of Lactobacillus kunkeei and two yeasts, Starmeralla magnolia MP-2 and Zygosaccharomyces siamensis MP-14, to compare it with commercially available BB.…”

Candida phyllophila sp. nov. and Candida vitiphila sp. nov., two novel yeast species from grape phylloplane in Thailand

“…The nucleotide sequences of the ITS regions of the three strains (K59 T , K60 and K70 T ) were also analyzed, but only the sequence of strain K70 T was obtained and deposited as AB736148. The ITS direct sequencing failed for strains K59 T and K60 because the chromatograms always showed overlapped peaks in nearly all positions of DNA strands, although a single band of PCR product was detected, showing that these strains might have heterogenous ITS rRNA gene copies (Egli and Henick-Kling, 2001;Saksinchai et al, 2012). In the ITS region, the sequences of strain K70 T exhibited 6.6% nucleotide substitutions (23 nucleotide substitutions and 4 gaps out of 349 nt) from its closest described species, C. asparagi.…”