Two new species of land planarians (Platyhelminthes, Tricladida, Geoplanidae) from protected areas in the southern extreme of the Paranaense Rainforest, Argentina

Trying to take over the world: Potential distribution of Obama nungara (Platyhelminthes: Geoplanidae), the Neotropical land planarian that has reached Europe

“…It is well known that land planarians, including O. nungara , harbour parasites, mainly nematode larvae, in different parts of the body (Negrete & Brusa, 2016, 2017; Negrete et al., 2019). In general, planarian specialists are not trained in identifying these nematodes, therefore, we simply describe their location in organs as supplementary information as a case of parasitism.…”

“…Sagittal and transverse serial sections, at 7 µm thick intervals, of the body regions above mentioned, were performed using a retracting rotary microtome. The histological sections were affixed with glycerinated albumin onto glass slides placed on a hotplate and stained using a modification of the Masson's trichrome method (Negrete, Díaz Gira, & Brusa, 2019). The histological preparations were observed using an optical microscope and the anatomical and histological features were compared with specimens studied by Carbayo et al (2016) and Lago-Barcia et al (2015, 2019.…”

Integrative taxonomy of land planarians (Platyhelminthes: Geoplanidae) from the Andean‐Patagonian Forests from Argentina and Chile, with the erection of two new genera

“…After euthanasia, they were fixed with 10% formalin and, subsequently, preserved in 70% ethanol. Different portions of two specimens of G. valdiviana and sixteen specimens of A. ruca were embedded in Paraplast©, serially sectioned at intervals of 4–6 μm with a microtome and stained with a modified Masson's trichrome stain (Negrete et al., 2019). The relative thickness of the cutaneous musculature in relation to body height (CMI, cutaneous muscular index) was measured at the pre‐pharyngeal region, following Froehlich (1955).…”

“…After euthanasia, they were fixed with 10% formalin and, subsequently, preserved in 70% ethanol. Different portions of two specimens of G. valdiviana and sixteen specimens of A. ruca were embedded in Paraplast©, serially sectioned at intervals of 4-6 μm with a microtome and stained with a modified Masson's trichrome stain (Negrete et al, 2019).…”

Planárias terrestres (Platyhelminthes, Geoplanidae) do Chile: taxonomia integrativa e descoberta de novos táxons endêmicos

“…As subfamílias Bipaliinae, Microplaninae e Rhynchodeminae possuem distribuição cosmopolita, enquanto que a subfamília Geoplaninae é exclusiva da região Neotropical, com representantes nas regiões à leste e à oeste da Cordilheira dos Andes (Carbayo, et al, 2013;Grau & Carbayo, 2010, Froehlich, 1957Winsor et al 1998 Schirch (1929), Riester (1938), Hyman (1938Hyman ( , 1939Hyman ( , 1941Hyman ( , 1955Hyman ( , 1962, Marcus (1951), Du Bois Reymond- Marcus (1951a), Froehlich (1955aFroehlich ( , b, 1956aFroehlich ( , b, 1959Froehlich ( , 1967, E. M. Froehlich (1955Froehlich ( , 1978, E. M. Froehlich e Froehlich (1972), Carbayo e Leal-Zanchet ( 2003), Leal-Zanchet e Souza ( 2003), Carbayo et. al., (2013), , Negrete et al, (2010Negrete et al, ( , 2011Negrete et al, ( , 2014Negrete et al, ( , 2015Negrete et al, ( , 2019.…”