Two new secondary metabolites isolated from Avena sativa L. (Oat) seedlings and their effects on osteoblast differentiation

Woo, So-Yeun; Lee, Kwang-Sik; Shin, Hye-Lim; Kim, Shin Hye; Lee, Mi-Ja; Kim, Ho; Ham, Hyeonmi; Lee, Dong Hoon; Choi, Sik‐Won; Seo, Woo Duck

doi:10.1016/j.bmcl.2020.127250

Cited by 8 publications

(6 citation statements)

References 21 publications

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“…The crude extract was re-extracted with 20 mL of MeOH for 5 h and filtered through a 0.2-μm RC syringe filter. Steroidal saponin content was determined using a UPLC-ELSD (Waters) system [6,7]. The column was a Halo C-18 column (2.7 μm, 100 mm × 2.1 mm inner diameter), the temperature was 35 °C, and the solvents were mobile phase A (0.1% formic acid in distilled water, v/v) and mobile phase B (100% acetonitrile).…”

Section: Determination Of Steroidal Saponin Contentmentioning

confidence: 99%

“…It contains phytochemicals such as steroidal saponins, avenanthramides (AVAs), phenolic acids, tocols, and flavonoids, that benefit human health; certain saponins accumulate in sprouts rather than seeds [6]. Oat sprouts prevent bone disorders [7], and wheat sprouts help alleviate obesity and diabetes [4]. To enhance germination and growth of and increase bioactive compound content in sprouts, physical and chemical methods [8] and application of LED lights and cold plasma have been evaluated as pretreatment methods in the field of agricultural science [9,10].…”

Section: Introductionmentioning

confidence: 99%

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Cold Plasma Treatment Increases Bioactive Metabolites in Oat (Avena sativa L.) Sprouts and Enhances In Vitro Osteogenic Activity of their Extracts

Lee

et al. 2022

Plant Foods Hum Nutr

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Cold plasma treatment has been studied to enhance the germination, growth, and bioactive phytochemical production in crops. Here, we aimed to investigate the effects of cold plasma treatment on the growth, bioactive metabolite production, and protein expression related to the physiological and osteogenic activities of oat sprouts. Oat seeds were soaked for 12 h, and then exposed to plasma for 6 min/day for 3 days after sowing. Plasma exposure did not significantly change the growth of oat sprouts; however, increased the content of bioactive metabolites. A single exposure for 6 min on the first day (T-1) increased the content of free amino acids (39.4%), γ-aminobutyric acid (53%), and avenacoside B (23%) compared to the control. Hexacosanol content was the highest in T-3 (6 min exposure on each day for 3 days), 28% higher than that in the control. Oat sprout extracts induced the phosphorylation of adenosine 5′-monophosphate-activated protein kinase and osteoblast differentiation was enhanced by increasing the alkaline phosphatase (ALP) activity; all these effects were induced by plasma treatment. Avenacoside B content was positively correlated with ALP activity (r = 0.911, p < 0.1). These results suggest that plasma treatment has the potential to improve the value of oat sprouts and that it may be used in food fortification to enhance nutritional value for promoting human health.

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Section: Determination Of Steroidal Saponin Contentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cold Plasma Treatment Increases Bioactive Metabolites in Oat (Avena sativa L.) Sprouts and Enhances In Vitro Osteogenic Activity of their Extracts

Lee

et al. 2022

Plant Foods Hum Nutr

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“…12 Moreover, saponins have been reported as natural substances with the potential to reduce methane gas production in ruminants. 13 Oats contain two types of saponins: Triterpenoid saponins, including avenacins A 1 , A 2 , B 1 , B 2 , and C−K, have been reported in oat bran and roots, 14,15 whereas steroidal saponins, avenacosides A−C, and their derivatives have been reported in oat shoots, 16,17 grains, 18−20 husks, 19 brans, 14,21,22 and aerial parts 23,24 and they have bioactivities, such as antibacterial, 17 antifungicidal, 15,16 osteogenic, 20 and anticancer. 22,24 Analytical methods for these compounds have mainly focused on NMR and MS analysis, but recently, high-resolution mass spectrometric methods, such as QToF-MS, have the efficiency to isolate and identify numerous components.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Seven New Steroidal Saponins from Korean Oat Cultivars by UPLC-QTOF-MS and UPLC-MS/MS

Kwon,

Lee,

Kim

et al. 2024

ACS Omega

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Oat saponins are composed of triterpenoid and steroidal saponins, and their potential biological activities, such as antibacterial, antifungicidal, osteogenic, and anticancer activities, have been reported. In this study, qualitative and quantitative analyses of oat saponins were conducted by using UPLC-QToF-MS and UPLC-Triple Q-MS/MS. A total of 22 saponins were analyzed in seven Korean oat cultivars. Among them, 7 saponins were identified as new compounds in this source, which were tentatively confirmed as nuatigenin-type saponins with 26-O-diglucoside and 3-Omalonylglucoside forms and (25S)-furost-5-en-3β,22,26-triol-type saponins. In addition, the total content of these saponins ranged from 70.61 to 141.38 mg/100 g dry weight, and it was affected by the type of oat cultivar and the presence or absence of hulling. These detailed profiles will be suggested as fundamental data for breeding superior oat cultivars, evaluating of related products, and various industries.

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“…For these above reasons, several researchers have recently documented the various biological properties such as antioxidant, antimicrobial, antidiabetic, anti-inflammatory, antiplatelet, and antiparasitic effects [ 20 , 21 ]. In addition, we have recently reported that the OSs exhibited anti-osteoporotic activity [ 22 ]. However, to the best of our knowledge, no studies have performed comparative analyses of policosanol derivatives and biological abilities in various cultivars of OSs.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Policosanols Related to Growth Times from the Seedlings of Various Korean Oat (Avena sativa L.) Cultivars and Screening for Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Activation

Lee

Woo

Ahn

et al. 2022

Plants

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The objectives of this research were to evaluate the policosanol profiles and adenosine-5′-monophosphate-activated protein kinase (AMPK) properties in the seedlings of Korean oat (Avena sativa L.) cultivars at different growth times. Nine policosanols in the silylated hexane extracts were detected using GC-MS and their contents showed considerable differences; specifically, hexacosanol (6) exhibited the highest composition, constituting 88–91% of the total average content. Moreover, the average hexacosanol (6) contents showed remarkable variations of 337.8 (5 days) → 416.8 (7 days) → 458.9 (9 days) → 490.0 (11 days) → 479.2 (13 days) → 427.0 mg/100 g (15 days). The seedlings collected at 11 days showed the highest average policosanol content (541.7 mg/100 g), with the lowest content being 383.4 mg/100 g after 5 days. Interestingly, policosanols from oat seedlings grown for 11 days induced the most prevalent phenotype of AMPK activation in HepG2 cells, indicating that policosanols are an excellent AMPK activator.

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Two new secondary metabolites isolated from Avena sativa L. (Oat) seedlings and their effects on osteoblast differentiation

Cited by 8 publications

References 21 publications

Cold Plasma Treatment Increases Bioactive Metabolites in Oat (Avena sativa L.) Sprouts and Enhances In Vitro Osteogenic Activity of their Extracts

Cold Plasma Treatment Increases Bioactive Metabolites in Oat (Avena sativa L.) Sprouts and Enhances In Vitro Osteogenic Activity of their Extracts

Characterization of Seven New Steroidal Saponins from Korean Oat Cultivars by UPLC-QTOF-MS and UPLC-MS/MS

Comparative Analysis of Policosanols Related to Growth Times from the Seedlings of Various Korean Oat (Avena sativa L.) Cultivars and Screening for Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Activation

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