Two new flavonoids and other constituents in licorice root. Their relative astringency and radical scavenging effects.

Hatano, Tsutomu; Kagawa, Harumi; Yasuhara, Taeko; Okuda, Takuo

doi:10.1248/cpb.36.2090

Cited by 1,165 publications

(650 citation statements)

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“…DPPH radical scavenging activity The scavenging effect of leaf extracts on DPPH radicals was determined according to the method of Hatano et al (1988). Various concentrations of sample (4 ml) were mixed with 1 ml of methanolic solution containing DPPH radicals, resulting in the final concentration of DPPH being 0.2 mM.…”

Section: Methodsmentioning

confidence: 99%

In vitro free radical scavenging and DNA damage protective property of Coriandrum sativum L. leaves extract

Harsha

Anilakumar

2012

J Food Sci Technol

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Coriandrum sativum L. (coriander), an everyday spice in the Indian kitchen is known to add flavor to the cuisine. It is an annual herb belonging to the Apiaceae (Umbellifera) family. The hydro-alcohol extract of Coriandrum sativum L. at the dose of 1 mg/ml was subjected to a series of in vitro assays viz. 2, 2′-diphenyl-1-picrylhydrazyl, lipid peroxidation by thiobarbituric acid, reducing power and nitric oxide (NO) radical scavenging in order to study its antioxidant efficacy in detail. The amount of flavonoids in 70% ethanol extract was found to be 44.5 μg and that of the total phenols was 133.74 μg gallic acid equivalents per mg extract. The extracts of the leaves showed metal chelating power, with IC 50 values, 368.12 μg/ ml where as that of standard EDTA was 26.7 μg/ml. The IC 50 values for 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid radical scavenging was 222 μg/ml where as that of standard ascorbic acid was 22.6 μg/ml. The NO scavenging activity of the extract of the leaves showed IC 50 value of 815.6 μg/ml; at the same time the standard BHA had 49.1 μg/ml. All the plant extracts provided DNA damage protection; however, the protection provided at the dose of 8 μg/ml was comparable to that of standard gallic acid. The Coriandrum sativum leaf extract was able to prevent in vitro lipid peroxidation with IC 50 values; 589.6 μg/ml where as that of standard BHA was 16.3 μg/ml. Our results also showed significant ferric reducing power indicating the hydrogen donating ability of the extract. This study indicated the potential of the leaf extract as a source of natural antioxidants or nutraceuticals that could be of use in food industry with potential application to reduce oxidative stress in living system.

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Section: Methodsmentioning

confidence: 99%

In vitro free radical scavenging and DNA damage protective property of Coriandrum sativum L. leaves extract

Harsha

Anilakumar

2012

J Food Sci Technol

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“…The DPPH radical-scavenging assay was used to determine the antioxidant activity of extracts by different methods according to a previously established procedure [Hatano et al 1988] with minor modifi cations. Extract solution (0.1 mL) in 95% methanol at various concentrations (10, 50, 100 ppm) was mixed with 3.0 mL of methanolic solution of DPPH [0.004% (w/v)].…”

Section: Dpph Radical Scavenging Assaymentioning

confidence: 99%

Optimized microwave-assisted extraction of 6-gingerol from Zingiber officinale Roscoeand evaluation of antioxidant activity in vitro

Liu¹,

Zhou²,

Zhao³

et al. 2014

Acta Sci Pol Technol Aliment

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Background. 6-Gingerol is one of the most pharmacologically active and abundant components in ginger, which has a wide array of biochemical and pharmacologic activities. In recent years, the application of microwave-assi sted extraction (MAE) for obtaining bioactive compounds from plant materials has shown tremendous research interest and potential. In this study, an effi cient microwave-assisted extraction (MAE) technique was developed to extract 6-gingerol from ginger. The extraction effi ciency of MAE was also compared with conventional extraction techniques. Material and methods. Fresh gingers (Zingiber officinale Rosc.) were harvested at commercial maturity (originally from Shandong, laiwu, China). In single-factor experiments for the recovery of 6-gingerol, proper ranges of ratio of liquid to solid, ethanol proportion, microwave power, extraction time were determined. Based on the values obtained in single-factor experiments, a Box-Behnken design (BBD) was applied to determine the best combination of extraction variables on the yield of 6-gingerol. Results. The optimum extraction conditions were as follows: mi crowave power 528 W, ratio of liquid to solid 26 mL·g -1 , extraction time 31 s and ethanol proportion 78%. Furthermore, more 6-gingerol and total polyphenols contents were extracted by MAE than conventional methods including Maceration (MAC), Stirring Extraction (SE), Heat reflux extraction (HRE), Ultrasound-assisted extraction (UAE), as well as the antioxidant capacity. Conclusion. Microwave-assisted extraction showed obvious advantages in terms of high extraction efficiency and antioxidant activity of extract within shortest extraction time. Scanning electron microscopy (SEM) images of ginger powder materials after different extractions were obtained to provide visual evidence of the disruption effect. To our best knowledge, this is the fi rst report about usage of MAE of 6-gingerol extraction from ginger, which could be referenced for the extraction of other active compounds from herbal plants.

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“…It has been mentioned that antioxidant activity of plants might be due to their phenolic compounds. Flavonoids are a group of polyphenolic compounds with known properties which include free radical scavenging, inhibition of hydrolytic and oxidative enzymes and anti-inflammatory action [3,4]. The presence of polyphenolic compound in the selected herbal plants prompted us to study the free radical scavenging activity.…”

Section: A Phytochemical and Mineral Analysismentioning

confidence: 99%

“…Recently there has been an upsurge of interest in the therapeutic potentials of medicinal plants as antioxidants in reducing such free-radical-induced tissue injury. Many plant extracts and phytochemicals have shown to have free radical scavenging properties but generally there is still a demand to find more information concerning the antioxidant potential of plant species [3,4].…”

Section: Introductionmentioning

confidence: 99%

Screening of Antioxidant, Anti-tumor and Antimicrobial Herbal Drugs/Diets from Some Myanmar Traditional Herbs

Mon¹,

Maw²,

Oo³

2011

IJBBB

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Abstract-Fractions of ethanolic extracts of three kinds of Myanmar herbal plants were used to screen for their antioxidant, anti-tumor and antimicrobial activities in this study. Free radical scavenging activities were showed with EC50 values and C. hirsutus showed the best activity among them. Moreover, not only C. hirsutus but also A. japonica showed potential anti-tumor activity at 100ppm dosage according to results from bioassay with carrot discs infected with Agrobacterium tumefaciens after three weeks incubation at 28±2˚C. From the preliminary phytochemical and mineral analyses, the results showed absence of cyanogenic glycosides, lead (Pb) and arsenic (As) in these plants and these plants are potentially safe to use as drugs or as diets. Although all kinds of plants extracts showed no toxicity by using in vitro brine shrimp larvae lethal toxicity test, A. japonica was selected to do further experiments as it has highest LC50 value among these three plants. In in vivo toxicity test with mice model, there is no toxic of A. japonica extract to the mice up to 2500mg/kg/day dosage indicating its safety for mammals. According to antagonistic activities against nine different species of food borne and human pathogenic microorganisms by the agar-well diffusion method, the methanol fraction of crude extract of the leaves of A. japonica showed most significant on the food borne pathogenic bacteria Shigella boydii and a zone of inhibition of 34mm in diameter. Ethyl acetate fractions also showed best results against other pathogens. The MIC value was in the ranged from 0.625 to 5.0mg/ml and the MBC value was in the ranged from 0.625 to 10.0mg/ml for these tested microorganisms and this indicated the distinct growth inhibition and wider spectrum of their potential antimicrobial activity. Therefore, the research clearly indicates that these herbal plants of Myanmar's dry farm land are potentially advantageous for human health as herbal drugs/ diets or as herbal food preservative.

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Two new flavonoids and other constituents in licorice root. Their relative astringency and radical scavenging effects.

Cited by 1,165 publications

References 0 publications

In vitro free radical scavenging and DNA damage protective property of Coriandrum sativum L. leaves extract

In vitro free radical scavenging and DNA damage protective property of Coriandrum sativum L. leaves extract

Optimized microwave-assisted extraction of 6-gingerol from Zingiber officinale Roscoeand evaluation of antioxidant activity in vitro

Screening of Antioxidant, Anti-tumor and Antimicrobial Herbal Drugs/Diets from Some Myanmar Traditional Herbs

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