Two new cellulolytic fungal species isolated from a 19th-century art collection

Coronado-Ruíz, Carolina; Avendaño, Roberto; Escudero-Leyva, Efraín; Conejo-Barboza, Geraldine; Chaverri, Priscila; Chavarría, Max

doi:10.1038/s41598-018-24934-7

Cited by 60 publications

(43 citation statements)

References 48 publications

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“…Among data for fully identical to the reference ITS sequences, several strains of this species were isolated from the surface of the art works: P. сhrysogenum FX5 (JX136719) and P. сhrysogenum FX43 (KJ780803) from wall paintings; P. сhrysogenum DTO 149 B5 (JX997027) from oil painting; P. сhrysogenum H09-022 (KC009774.1) and P. chrysogenum WL5-2B (MF422150) from 19 th century collection items. Many of these strains were isolated from both walls and air samples taken in various parts of buildings, museums, tombs, industrial premises, and termitaries (Tables 2, 3) [9,13,14].…”

Section: Resultsmentioning

confidence: 99%

Xerotolerant strain of Penicillium chrysogenum MF18_10 isolated from the damaged walls of Saint Sophia’s Cathedral, Kyiv

Lutsenko¹,

Polishchuk²,

Hong³

et al. 2019

Fakt. eksp. evol. org.

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Aim. The aim of this work is to determine the taxonomic position of the fungal strain MF18_10 isolated from the damaged wall with medieval painting of St. Sophia’s Cathedral (Kyiv, Ukraine). Methods. The fungus was isolated on selective medium for xerophilic fungi (70% sucrose Czapek agar). Мacro- and micro-morphological phenotypic characterization was carried out using light and scanning electron microscopy. Molecular-biological identification was performed using nucleotide sequences of the ITS-fragment. Results. On the basis of phenotypical characteristics of the fungal isolate MF18_10, it was established its affiliation to the genus Penicillium. ITS analysis revealed that the isolate belongs to the species P. chrysogenum demonstrating 100% identity with other 78 P. chrysogenum strains in GenBank database including the type strains NR_077145 and AY373902, also sharing several distinct differences in substitutions, deletions and insertions within this group. Conclusions. The isolated xerotolerant fungus was identified as P. chrysogenum, the typical representative of the indoor environments and dust, and also common for mycobiota of damaged historic-cultural artifacts. The differences in the analyzed P. chrysogenum ITS primary structures did not correlate with the source of isolation. Keywords: Penicillium chrysogenum, xero-tolerant fungi, ITS, scanning electron microscopy.

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Section: Resultsmentioning

confidence: 99%

Xerotolerant strain of Penicillium chrysogenum MF18_10 isolated from the damaged walls of Saint Sophia’s Cathedral, Kyiv

Lutsenko¹,

Polishchuk²,

Hong³

et al. 2019

Fakt. eksp. evol. org.

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“…The CBD pathogen was placed along with non-CBD causing isolates into two subspecies within C. kahawae. This taxonomic framework led, in recent years, to the identification of "C. kahawae" (no subspecies given) in other hosts and regions [32][33][34][35], raising concern among plant pathologists dealing with coffee diseases, especially when the spread of C. kahawae out of Africa is so feared [13].…”

Section: Discussionmentioning

confidence: 99%

Pathological, Morphological, Cytogenomic, Biochemical and Molecular Data Support the Distinction between Colletotrichum cigarro comb. et stat. nov. and Colletotrichum kahawae

Cabral

Azinheira

Talhinhas

et al. 2020

Plants

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The genus Colletotrichum has witnessed tremendous variations over the years in the number of species recognized, ranging from 11 to several hundreds. Host-specific fungal species, once the rule, are now the exception, with polyphagous behavior regarded as normal in this genus. The species Colletotrichum kahawae was created to accommodate the pathogens that have the unique ability to infect green developing coffee berries causing the devastating Coffee Berry Disease in Africa, but its close phylogenetic relationship to a polyphagous group of fungi in the C. gloeosporioides species complex led some researchers to regard these pathogens as members of a wider species. In this work we combine pathological, morphological, cytogenomic, biochemical, and molecular data of a comprehensive set of phylogenetically-related isolates to show that the Coffee Berry Disease pathogen forms a separate species, C. kahawae, and also to assign the closely related fungi, previously in C. kahawae subsp. cigarro, to a new species, C. cigarro comb. et stat. nov. This taxonomic clarification provides an opportunity to link phylogeny and functional biology, and additionally enables a much-needed tool for plant pathology and agronomy, associating exclusively C. kahawae to the Coffee Berry Disease pathogen.

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“…Namely, the strains identified as R. mucilaginosa WUT10 and WUT60, unlike WUT167, exhibited growth up to 30 • C. Similarly, for two Sporobolomyces roseus strains, the maximum growth temperature varied between 25 • C and 30 • C for WUT61 and WUT182, respectively. Although many enzymes with industrial applications are produced using yeasts [28][29][30], new and safe sources of enzyme preparations are still being sought. Therefore, the ability of newly isolated strains to produce extracellular enzymes was also verified ( Table 2).…”

Section: Physiological and Biochemical Characteristic Of Yeastsmentioning

confidence: 99%

Carotenoid-Producing Yeasts: Identification and Characteristics of Environmental Isolates with a Valuable Extracellular Enzymatic Activity

et al. 2019

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Sixteen cold-adapted reddish-pigmented yeast strains were obtained from environmental samples. According to the PCR-based detection of classical yeast markers combined with phylogenetic studies, the yeasts belong mainly to the genera Rhodotorula, Sporobolomyces and Cystobasidium, all within the subphylum Pucciniomycotina. All strains produced carotenoids within a 0.25–10.33 mg/L range under non-optimized conditions. Noteworthily, among them, representatives of the Cystobasidium genus were found; of particular value are the strains C. laryngis and C. psychroaquaticum, poorly described in the literature to date. Interestingly, carotenoid production with representatives of Cystobasidium was improved 1.8- to 10-fold at reduced temperature. As expected, most of the isolated yeasts biosynthesized extracellular lipases, but within them also one proteolytic and four cellulolytic strains were revealed. We succeeded in isolating strain Cystofilobasidium macerans WUT145 with extraordinarily high cellulolytic activity at 22°C (66.23 ± 0.15 µmol/mg protein·min) that is described here for the first time. Consequently, a set of yeasts capable of producing both carotenoids and extracellular enzymes was identified. Taking into account those abilities, the strains might be applicable for a development of carotenoids production on an agro-industrial waste, e.g., lignocellulose.

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Two new cellulolytic fungal species isolated from a 19th-century art collection

Cited by 60 publications

References 48 publications

Xerotolerant strain of Penicillium chrysogenum MF18_10 isolated from the damaged walls of Saint Sophia’s Cathedral, Kyiv

Xerotolerant strain of Penicillium chrysogenum MF18_10 isolated from the damaged walls of Saint Sophia’s Cathedral, Kyiv

Pathological, Morphological, Cytogenomic, Biochemical and Molecular Data Support the Distinction between Colletotrichum cigarro comb. et stat. nov. and Colletotrichum kahawae

Carotenoid-Producing Yeasts: Identification and Characteristics of Environmental Isolates with a Valuable Extracellular Enzymatic Activity

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