Pathological, Morphological, Cytogenomic, Biochemical and Molecular Data Support the Distinction between Colletotrichum cigarro comb. et stat. nov. and Colletotrichum kahawae

Cabral, Amílcar; Azinheira, H. G.; Talhinhas, Pedro; Batista, Dora; Ramos, Ana Paula; Silva, Maria do Céu; Oliveira, Helena; Várzea, Vítor

doi:10.3390/plants9040502

Cited by 25 publications

(14 citation statements)

References 68 publications

(126 reference statements)

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“…According to our knowledge, this study is the first report of C. boninense , C. fructicola , and C. perseae affecting olive trees. Other studies have described another six Colletotrichum species associated with this crop, C. aenigma , C. cigarro , C. karstii , C. queenslandicum , C. lupini , and C. rhombiborme [ 4 , 5 , 23 , 77 , 78 ]. Although many other woody crops are affected by numerous species of Colletotrichum [ 21 , 54 , 79 , 80 ], the olive tree is one of the plant species affected by the most remarkable diversity of taxa of this fungal genus with 18 species.…”

Section: Discussionmentioning

confidence: 99%

Diversity of Colletotrichum Species Associated with Olive Anthracnose Worldwide

Moral

Agustí-Brisach

Raya

et al. 2021

JoF

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Olive anthracnose caused by Colletotrichum species causes dramatic losses of fruit yield and oil quality worldwide. A total of 185 Colletotrichum isolates obtained from olives and other hosts showing anthracnose symptoms in Spain and other olive-growing countries over the world were characterized. Colony and conidial morphology, benomyl-sensitive, and casein-hydrolysis activity were recorded. Multilocus alignments of ITS, TUB2, ACT, CHS-1, HIS3, and/or GAPDH were conducted for their molecular identification. The pathogenicity of the most representative Colletotrichum species was tested to olive fruits and to other hosts, such as almonds, apples, oleander, sweet oranges, and strawberries. In general, the phenotypic characters recorded were not useful to identify all species, although they allowed the separation of some species or species complexes. ITS and TUB2 were enough to infer Colletotrichum species within C. acutatum and C. boninense complexes, whereas ITS, TUB2, ACT, CHS-1, HIS-3, and GADPH regions were necessary to discriminate within the C. gloesporioides complex. Twelve Colletotrichum species belonging to C. acutatum, C. boninense, and C. gloeosporioides complexes were identified, with C. godetiae being dominant in Spain, Italy, Greece, and Tunisia, C. nymphaeae in Portugal, and C. fioriniae in California. The highest diversity with eight Colletotrichum spp. was found in Australia. Significant differences in virulence to olives were observed between isolates depending on the Colletotrichum species and host origin. When other hosts were inoculated, most of the Colletotrichum isolates tested were pathogenic in all the hosts evaluated, except for C. siamense to apple and sweet orange fruits, and C. godetiae to oleander leaves.

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Section: Discussionmentioning

confidence: 99%

Diversity of Colletotrichum Species Associated with Olive Anthracnose Worldwide

Moral

Agustí-Brisach

Raya

et al. 2021

JoF

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“…kahawae and C . cigarro were adopted from the study conducted by Cabral et al [ 33 ]. Phylogenetic tree showed that present isolates were clustered together with C .…”

Section: Resultsmentioning

confidence: 99%

An account of Colletotrichum species associated with anthracnose of Atractylodes ovata in South Korea based on morphology and molecular data

et al. 2022

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Ovate-leaf atractylodes (OLA) (Atractylodes ovata) is a well-known medicinal plant in Korea; its dried rhizome and root extracts are used in herbal medicine. However, anthracnose is a great challenge to the OLA cultivation in South Korea. Colletotrichum spp. is a major group of plant pathogens responsible for anthracnose on a range of economically important hosts. Its occurrence on OLA remains unresolved. To investigate the diversity, morphology, phylogeny, and biology of Colletotrichum spp., 32 fungal isolates were obtained from 30 OLA-affected leaves collected from five different farms, in two regions in South Korea, Mungyeong and Sangju. The phylogenetic analysis with four or five gene loci (ITS, TUB2, ACT, GAPDH, and CHS-1) along with morphology of 26 representative isolates delineated six previously known Colletotrichum species including C. fructicola, C. gloeosporioides sensu stricto (s.s), C. cigarro, C. plurivorum, C. siamense and C. sojae, and one new species, described here as C. ovataense. Amongst these species, C. gloeosporioides s.s. and C. plurivorum were the most prevalent species. A pathogenicity test on the detached leaves revealed that different Colletotrichum species presented a distinct degree of virulence, confirming Koch’s postulates. In this study, C. fructicola, C. cigarro, C. plurivorum, C. siamense, and C. sojae were reported from A. ovata for the first time, as the causal agent of ovate-leaf atractylodes anthracnose. Understanding the diversity and biology of the Colletotrichum species population will help in managing this disease.

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“…ciggaro (Weir et al ., 2012). However, both subspecies were recently classified to species level using a polyphasic approach (Cabral et al ., 2020). Another example is C .…”

Section: Discussionmentioning

confidence: 99%

Colletotrichum species causing anthracnose on avocado fruit in Mexico: Current status

et al. 2020

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Colletotrichum species cause anthracnose disease in tropical and subtropical fruit crops worldwide. Mexico is the main producer and exporter of avocado (Persea americana) globally and has yearly outbreaks of anthracnose on this crop. However, which specific Colletotrichum spp. cause these outbreaks in avocado‐producing regions remain uncertain; thus, the objective of the present study was to identify the species responsible. A survey performed in six production regions of Mexico yielded 232 isolates, from which a subset of 104 strains was selected based on morphological characteristics and origin. This subset was sequenced and haplotypes were analysed in the gapdh partial gene. Finally, 31 strains were identified through multilocus phylogenetic analyses using the sequences of the internal transcribed spacer region and six loci. This study revealed the presence of two species previously reported in Mexico (C. karsti and C. godetiae), three novel records in Mexico (C. siamense, C. fioriniae, and C. cigarro), four novel records on avocado (C. chrysophilum, C. jiangxiense, C. tropicale, and C. nymphaeae), and two novel lineages (Colletotrichum sp. 1 and Colletotrichum sp. 2). C. siamense was the most prevalent, while the species reported for the first time on avocado, including the novel lineages, were the least prevalent. C. karsti was the most widespread (four regions), followed by C. siamense, C. jiangxiense, and C. chrysophilum (three regions). Pathogenicity tests showed that all species caused anthracnose on avocado fruit. These findings will be useful for improving the management of avocado anthracnose outbreaks in Mexico.

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Pathological, Morphological, Cytogenomic, Biochemical and Molecular Data Support the Distinction between Colletotrichum cigarro comb. et stat. nov. and Colletotrichum kahawae

Cited by 25 publications

References 68 publications

Diversity of Colletotrichum Species Associated with Olive Anthracnose Worldwide

Diversity of Colletotrichum Species Associated with Olive Anthracnose Worldwide

An account of Colletotrichum species associated with anthracnose of Atractylodes ovata in South Korea based on morphology and molecular data

Colletotrichum species causing anthracnose on avocado fruit in Mexico: Current status

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