Two methods for glass surface modification and their application in protein immobilization

Qin, Ming; Hou, Sen; Wang, LiKai; Feng, Xisheng; Wang, Rui; Yang, Yan; Wang, Chen; Yu, Lei; Shao, Bin; Qiao, Mingqiang

doi:10.1016/j.colsurfb.2007.06.018

Cited by 108 publications

(90 citation statements)

References 53 publications

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“…WCA of modified surface was higher than the unmodified one. This could be due to the increase of the hydrophobicity as well as decrease of the wettability of the surface by the presence of aliphatic carbon chains (ethyl and propyl stretches) as a result of silanation (Qin et al 2007). …”

Section: Synthesis and Characterization Of Citrate-capped Aunpsmentioning

confidence: 99%

“…APTES functionalized PS plates and GS are used in the immobilization of antibodies for the detection of antigens. Similarly, oligonucleotide probes/DNA were used to detect target DNA sequences and single nucleotide polymorphisms (SNP) typing and assaying in the protein kinases activity (Dixit et al 2010;Qin et al 2007;Pack et al 2007;Hirayama et al 1996;Nikiforov et al 1994;Kim et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…The presence of functional groups and chemical status of elements, rough and smooth surface, hydrophobic and hydrophilic properties of materials was characterized by using X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements (North et al 2010;Qin et al 2007;Idage and Badrinarayanan 1998). However, these characterization methods are cost-effective, time consuming and they are not affordable for routine modification process in the view of commercialization.…”

Section: Introductionmentioning

confidence: 99%

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Gold nanoparticles assisted characterization of amine functionalized polystyrene multiwell plate and glass slide surfaces

et al. 2014

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We demonstrated citrate-capped gold nanoparticles assisted characterization of amine functionalized polystyrene plate and glass slide surfaces through AuNPs staining method. The effect of AuNPs concentration on the characterization of amine modified surfaces was also studied with different concentration of AuNPs (ratios 1.0-0.0). 3-Aminopropylyl triethoxy silane has been used as amine group source for the surface modification. The interactions of AuNPs on modified and unmodified surfaces were investigated using atomic force microscopy and the dispersibility, and the aggregation of AuNPs was analyzed using UV-visible spectrophotometer. Water contact angle measurement and X-ray photoelectron spectroscopy (XPS) were used to further confirmation of amine modified surfaces. The aggregation of AuNPs in modified multiwell plate leads to the color change from red to purple and they are found to be adsorped on the modified surfaces. Aggregation and adsorption of AuNPs on the modified surfaces through the electrostatic interactions and the hydrogen bonds were revealed by XPS analysis. Remarkable results were found even in the very low concentration of AuNPs (ratio 0.2). This AuNPs staining method is simple, cost-effective, less time consuming, and required very low concentration of AuNPs. These results can be read out through the naked eye without the help of sophisticated equipments.

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Section: Synthesis and Characterization Of Citrate-capped Aunpsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gold nanoparticles assisted characterization of amine functionalized polystyrene multiwell plate and glass slide surfaces

et al. 2014

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“…The values of contact angles were increased with increasing APTMS concentration because of the hydrophobicity of the surface increment with the deposited APTMS density. 43 However, the feature of the contact angle in Fig. 6 showed a difference from that of absorbance in Fig.…”

Section: Modulation Of Au Nanoparticle Deposition By Organosilanesmentioning

confidence: 87%

Gold Nanoparticle Monolayer Formation on a Chemically Modified Glass Surface

2009

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Physicochemical analyses of Au nanoparticle monolayer formation on a glass surface functionalized with 3-aminopropyltrimethoxysilane (APTMS) and ethyltrimethoxysilane (ETMS) were performed for fabricating a nanoarchitecture of metal nanoparticles using self-assembly. The Au nanoparticle surface density on the functionalized glass surface correlated linearly with the optical response because of Au-nanoparticle localized surface plasmon resonance (LSPR). This relation enabled assessment of the Au nanoparticle deposition. A Frumkin isotherm showed the deposition of Au nanoparticles on the APTMS or APTMS/ETMS functionalized glass surface. For an APTMS-derivatized surface, the affinity between the Au nanoparticles and the surface decreased with the lowering of the APTMS surface coverage. The interaction parameter exhibited attractive interaction of 30-nm-diameter Au nanoparticles; 12 nm particles were repulsive. Adsorption isotherm experiments using Au nanoparticles on glass surfaces with an APTMS and ETMS mixed layer suggest that hydrophobic interaction between Au nanoparticles' bare gold surfaces caused the attractive interaction among 30-nm-diameter nanoparticles.

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“…Glass has been one of the widely used substrates for protein chips. Qin et al (2007a) have compared two methods for protein immobilization on glass slides, one is the traditional method of silanization (Mezzasoma et al 2002); the other is the coating with the class II hydrophobin HFBI from Trichoderma reesei. The modified glass surfaces were characterized with X-ray photoelectron spectroscopy (XPS), water contact angle measurement (WCA) and immunoassay.…”

Section: Functional Surfaces Based On Hydrophobins Biofilmsmentioning

confidence: 99%