Gold nanoparticles assisted characterization of amine functionalized polystyrene multiwell plate and glass slide surfaces

Abstract

We demonstrated citrate-capped gold nanoparticles assisted characterization of amine functionalized polystyrene plate and glass slide surfaces through AuNPs staining method. The effect of AuNPs concentration on the characterization of amine modified surfaces was also studied with different concentration of AuNPs (ratios 1.0-0.0). 3-Aminopropylyl triethoxy silane has been used as amine group source for the surface modification. The interactions of AuNPs on modified and unmodified surfaces were investigated using atomic force microscopy and the dispersibility, and the aggregation of AuNPs was analyzed using UV-visible spectrophotometer. Water contact angle measurement and X-ray photoelectron spectroscopy (XPS) were used to further confirmation of amine modified surfaces. The aggregation of AuNPs in modified multiwell plate leads to the color change from red to purple and they are found to be adsorped on the modified surfaces. Aggregation and adsorption of AuNPs on the modified surfaces through the electrostatic interactions and the hydrogen bonds were revealed by XPS analysis. Remarkable results were found even in the very low concentration of AuNPs (ratio 0.2). This AuNPs staining method is simple, cost-effective, less time consuming, and required very low concentration of AuNPs. These results can be read out through the naked eye without the help of sophisticated equipments.

Highly Biocompatible Nanoparticles of Au@Fluorescent Polymers as Novel Contrast Agent for In Vivo Bimodality NIR Fluorescence/CT Imaging

“…The composition of the Au@FP nanoparticles was further tested by XPS spectra and FTIR and is shown in the Figures 1(f) and 1(g). The Au 4f high-resolution XPS pattern reveals that the binding energy values of Au4f 7/2 and Au4f 5/2 are 83.7 and 87.4 eV, respectively, consistent with the previous reports about Au nanoparticles [33, 34]. The Au4f, C1s, O1s, S2p, and N1s derived from Au nanoparticles and fluorescence polymers confirm the formation of Au@FP nanoparticles.…”

Enhancement of the Start-Up Time for Microliter-Scale Microbial Fuel Cells (µMFCs) via the Surface Modification of Gold Electrodes

“…Water molecules can interact with amine groups by forming hydrogen bonds, justifying the modification of the surface. The introduction of amine groups was also found to increase the hydrophilicity in other studies [ 36 ].…”

Scanometric Detection of Tomato Leaf Curl New Delhi Viral DNA Using Mono- and Bifunctional AuNP-Conjugated Oligonucleotide Probes

“…Glass slide (GS) surface was modified with amine functional groups using APTES followed in our earlier report. 27 In detail, glass slides were first treated with piranha solution (H 2 O 2 /H 2 SO 4 , 30:70 ratio) for 12 h, washed thoroughly with ddH 2 O, and dried under a stream of N 2 gas. For the silanization, the treated GSs were immersed in a solution containing 2.0% of APTES at room temperature (RT) for 30 min.…”