Two mechanisms for increasing milt volume in male goldfish,Carassius auratus

Zheng, Wenbin; Stacey, N. E.

doi:10.1002/(sici)1097-010x(19961101)276:4<287::aid-jez6>3.0.co;2-k

Cited by 34 publications

(12 citation statements)

References 26 publications

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“…On the next day, one male in each trio was tested at 0900 hours, one was tested at 1300 hours; the third male was not tested in a spawning trial, but was used to determine the effect of spawning on sperm stores. Approximately 1 h before each trial, two females were anaesthetized (0·05% 2‐phenoxyethanol; Syndel Laboratories Ltd, Qualicum Beach, British Columbia, Canada), weighed, measured ( L S ) and injected in the dorsal musculature with 200 ng g −1 prostaglandin F 2α to induce spawning behaviour (Zheng & Stacey, 1996). After recovery, the females were placed in separate 65 l pre‐trial test aquaria.…”

Section: Methodsmentioning

confidence: 99%

“…After stopping the trial, the fish were removed, the aquarium was thoroughly stirred for 3 min and a water sample of 250–1000 ml was collected (depending on the number of spawns). Males were weighed and measured ( L S ), and milt was collected into pre‐weighed capillary tubes as described by Zheng & Stacey (1996). After capillary tubes were weighed and centrifuged, milt volume was calculated by assuming milt has a specific gravity of 1 g cm −3 and spermatocrit was calculated as the sum of packed sperm length in all tubes divided by the sum of total milt length in all tubes; this measurement is a good predictor of the number of sperm cells per unit volume of milt (Bouck & Jacobson, 1976).…”

Section: Methodsmentioning

confidence: 99%

“…The first major objective in this study, therefore, was to determine if 17,20βP exposure increases ejaculate size in spawning male C. auratus . Although it can be predicted that the first ejaculates of pheromone‐exposed males should be greater than those of unexposed males, it is unclear whether this initial difference will be maintained during multiple spawnings, because, even without prior exposure to 17,20βP, males rapidly increase strippable sperm in response to spawning stimuli (Zheng & Stacey, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Large and persistent effect of a female steroid pheromone on ejaculate size in goldfish Carassius auratus

Hoysak

Stacey

2008

Journal of Fish Biology

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This study determined if ejaculate size in male goldfish Carassius auratus is increased by the female preovulatory steroid pheromone 4-pregnen-17,20b-diol-3-one (17,20bP), which previously has been shown to affect male behaviour and to increase sperm motility and stripped sperm number, and also to increase paternity in competitive spawning and competitive in vitro fertilization. Experimental males were exposed overnight to 17,20bP whereas control males were not. The morning following exposure, each male was placed with a reproductively active female and, after one to 20 spawning acts, aquarium water was sampled to quantify released sperm. Although exposure to 17,20bP induced a five-fold difference in the number of sperm that could be stripped, the median number of sperm in first ejaculates of pheromone-exposed males was >60 sixty times that of control males, a pheromonal effect on ejaculate size that persisted for at least 20 spawning acts. The magnitude of the pheromone effect on ejaculate size indicates that it is a critical component of C. auratus sperm allocation, and that examining this effect in concert with other factors (e.g. presence of competitors, male and female size and frequency of spawning) will reveal the contribution of the preovulatory pheromone to male fitness in this promiscuous species.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Large and persistent effect of a female steroid pheromone on ejaculate size in goldfish Carassius auratus

Hoysak

Stacey

2008

Journal of Fish Biology

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“…In addition, previous work has demonstrated that milt expression can be modulated by various social cues. Specifically, male goldfish increase their milt volume in response to sexual cues from females, including pheromonal cues (Zheng and Stacey, 1996), as well as in the presence of conspecific males (Stacey et al, 2001). Interestingly, these same social cues also cause a surge in circulating levels of T (Kobayashi et al, 1986;Stacey et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Testosterone rapidly increases ejaculate volume and sperm density in competitively breeding goldfish through an estrogenic membrane receptor mechanism

Mangiamele

Thompson

2012

Hormones and Behavior

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“…Simultaneously, behaviours of exposed males change in ways that facilitate attraction to imminent spawning partners and promote sperm competition with other males (Zheng et al , 1997; Poling et al , 2001). The key odorants mediating these preovulatory primer and releaser effects are the maturation inducing steroid (MIS), 4‐pregnen‐17,20 β ‐diol‐3‐one (17,20 β ‐P), and its sulphated and glucuronated conjugates, and androstenedione, as demonstrated by measurement of steroid release to the water (Stacey et al , 1989; Scott & Sorensen, 1994; Sorensen et al , 2005), electro‐olfactogram (EOG) recordings of olfactory sensitivity and specificity to these steroids (Sorensen et al , 1995), and the biological responses they induce when added to aquarium water (Dulka et al , 1987; Zheng & Stacey, 1996, 1997; Poling et al , 2001).…”

Section: Introductionmentioning

confidence: 99%

Male primer endocrine responses to preovulatory female cyprinids under natural conditions in Sweden

Stacey

Kraak

Olsén

2012

Journal of Fish Biology

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This study investigated two related aspects of male-female reproductive interactions in the family Cyprinidae: (1) whether ovulating female rudd Scardinius erythrophthalmus (subfamily Leuciscinae) induce endocrine and gonadal priming responses in conspecific males, a phenomenon which has been described only in species from the subfamily Cyprininae such as goldfish, Carassius auratus, crucian carp Carassius carassius and common carp, Cyprinus carpio and (2) whether the stimuli mediating these responses are species-specific. Field studies of three sympatric European cyprinids, two leuciscins (S. erythrophthalmus and white bream Blicca bjoerkna) and one cyprinin (C. carassius), were conducted on fishes captured in Sweden in the spawning season and held in net pens under natural conditions. As previously reported in C. carassius, male S. erythrophthalmus increased milt (sperm and seminal fluid) volume and plasma concentrations of the sperm maturation hormone 4-pregnen-17,20β-diol-3-one (17,20β-P) when they were held with female S. erythrophthalmus induced to ovulate by injection of Ovaprim (GnRH analogue plus dopamine antagonist). Male S. erythrophthalmus had larger milt volumes than male C. carassius prior to and following exposure to ovulatory conspecifics, but exhibited a smaller proportional milt increase in response to stimulation, suggesting species differences in sperm allocation at spawning. The presence of female S. erythrophthalmus and B. bjoerkna did not affect milt volumes of C. carassius under two experimental conditions: (1) ovulating S. erythrophthalmus and B. bjoerkna did not increase the milt volumes of C. carassius and (2) S. erythrophthalmus and B. bjoerkna did not interfere with the milt volume increase induced in male C. carassius by ovulating conspecifics. These results suggest that, as in C. auratus, C. carassius and C. carpio (subfamily Cyprininae), female S. erythrophthalmus (subfamily Leuciscinae) release a preovulatory pheromone that exerts priming effects on male hormones and sperm allocation. The findings also indicate that C. carassius discriminate between the reproductive odours of conspecifics and heterospecifics.

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Two mechanisms for increasing milt volume in male goldfish,Carassius auratus

Cited by 34 publications

References 26 publications

Large and persistent effect of a female steroid pheromone on ejaculate size in goldfish Carassius auratus

Large and persistent effect of a female steroid pheromone on ejaculate size in goldfish Carassius auratus

Testosterone rapidly increases ejaculate volume and sperm density in competitively breeding goldfish through an estrogenic membrane receptor mechanism

Male primer endocrine responses to preovulatory female cyprinids under natural conditions in Sweden

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