Two Major Medicinal Honeys Have Different Mechanisms of Bactericidal Activity

Kwakman, Paulus H. S.; Velde, Anje A. te; Boer, Leonie de; Vandenbroucke-Grauls, Christina M. J. E.; Zaat, Sebastian A. J.

doi:10.1371/journal.pone.0017709

Cited by 235 publications

(250 citation statements)

References 37 publications

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“…One of the potential agents responsible for biofilm destruction is bee (Apis melifera)-derived antibacterial peptide defensin-1 (Def-1), as it has been shown recently that antimicrobial cationic peptides destroy bacterial biofilm (Dosler & Mataraci, 2013;Dosler & Karaaslan, 2014). Def-1 is one of the main regular but quantitatively variable antibacterial components of honey (Kwakman et al, 2011;Majtan et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Sojka

Valachová

Bucekova

et al. 2016

Journal of Medical Microbiology

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Many clinically relevant biofilms are polymicrobial. Examining the effect of antimicrobials in a multispecies biofilm consortium is of great clinical importance. The goal of this study was to investigate the effect of different honey types against bacterial wound pathogens grown in multispecies biofilm and to test the antibiofilm activity of honey defensin-1 (Def-1) in its recombinant form. A modified Lubbock chronic wound biofilm formed by four bacterial species (Staphylococcus aureus, Streptococcus agalactiae, Pseudomonas aeruginosa and Enterococcus faecalis) was used for evaluation of honey and recombinant bee-derived Def-1 antibiofilm efficacy. Recombinant Def-1 was prepared by heterologous expression in Escherichia coli. We showed that different types of honey (manuka and honeydew) were able to significantly reduce the cell viability of wound pathogens (Staphylococcus aureus, Streptococcus agalactiae and Pseudomonas aeruginosa) in mature polymicrobial biofilm. None of the tested honeys showed the ability to eradicate Enterococcus faecalis in biofilm. In addition, recombinant Def-1 successfully reduced the viability of Staphylococcus aureus and Pseudomonas aeruginosa cells within established polymicrobial biofilm after 24 and 48 h of treatment. Interestingly, recombinant Def-1 did not affect the viability of Streptococcus agalactiae cells within the biofilm, whereas both natural honeys significantly reduced the viable bacteria. Although Enterococcus faecalis was highly resistant to Def-1, Def-1 significantly affected the biofilm formation of Enterococcus faecalis and Streptococcus agalactiae after 24 h of treatment, most likely by inhibiting its extracellular polymeric substances production. In conclusion, our study revealed that honey and Def-1 are effective against established multispecies biofilm; however, Enterococcus faecalis grown in multispecies biofilm was resistant to both antimicrobials.

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Section: Introductionmentioning

confidence: 99%

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Sojka

Valachová

Bucekova

et al. 2016

Journal of Medical Microbiology

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“…Several authors also studied the correlations between color and antibacterial activities with content of the bioactive compounds of honey. Various studies have been conducted to investigate the antimicrobial effects [13][14][15]. Limited studies have been done on Algerian honey.…”

Section: Introductionmentioning

confidence: 99%

Colour Intensity, Polyphenol Content and Antibacterial Capacity of Unheated and Heat-Treated Sahara Honey

Ahmed¹,

Khiati²,

Saad³

et al. 2016

J Food Process Technol

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The objective of this research work was to evaluate the effect of heat processing on antibacterial capacity of Sahara honey (SH). Various thermal treatments were carried out at 25°C, 50°C, 75°C and 100°C for 15 min, 30 min, and 60 min, and the parameters were determined: colour intensity, phenolic contents and antibacterial activity of two species of bacteria (Staphylococcus aureus and Pseudomonas aeruginosa). The total phenolic contents in honey samples varied from 0.55 and 1.54 mg of gallic acid equivalent (GAE) in gram of honey. MIC values respectively 3.12 to 12.5 mg/ml and zone of growth inhibition respectively 2.65 mm to 19 mm. Exposures of SH at 75°C and 100°C, no inhibition of Staphylococcus aureus and Pseudomonas aeruginosa growth was detected. Statistical analysis demonstrated positive correlations between color intensity and polyphenol contents and negative correlations with antibacterial capacity. Data from present results revealed that unheated and heat-treated Sahara honey showed growth inhibitory effect against Staphylococcus aureus and Pseudomonas aeruginosa with variable degrees.

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“…Nilgiris honeys showed Zone Diameter of Inhibition (ZDIs) of 20-21 mm for S. aureus, 15-16 mm for P. aeruginosa and 13-14 mm for E. coli (Rajeswari et al, 2010). RS and Manuka honeys killed B. subtilis, E. coli, P. aeruginosa and S. aureus after 24 h of incubation (Kwakman et al, 2011) using disc diffusion method. In this study using disc diffusion method, it was observed that Tualang and Acacia honeys showed higher inhibitory activity compared to Nilgiris honeys, but comparable with the activity from Egyptian clover honey.…”

Section: Ajasmentioning

confidence: 99%

Potency of Honey as Antibacterial Agent Against Multiple Antibiotic Resistant Pathogens Evaluated by Different Methods

Aween¹,

Hassan²,

Huda-Faujani³

et al. 2014

American Journal of Applied Sciences

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Honey is rich with complex natural components which could be useful as antibacterial agents or as preservative. Honey contains high concentration of sugars, low amount of water, high osmolality and often dark colour which influence its antibacterial activity. Disc diffusion, well method, micro dilution assay are methods commonly used to determine the antibacterial activity of honey. In this study, microtiter and microbial plate count were included to ascertain the potency of honey as antibacterial agent against multiple antibiotic resistant pathogenic bacteria (Staphylococcus aureus, Salmonella Typhimurium, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa) with concentration of 0.2 g mL −1 . Results found that well diffusion method tends to give higher inhibitory zone than disc diffusion method but there was no correlation among the bacteria was observed except for S. Typhimurium, E. coli (R = 0.310, 0.505 and 0.316, respectively). Nan photometer assay and microtiter plates assay showed comparable results with moderately strong correlation (R 2 = 0.681 and 0.767, respectively) for S. aureus and S. typhimurium, but poor correlation was found for E. coli, B. subtilis and P. aeruginosa (R 2 = 0.441, 0.308 and 0.383, respectively). Determining the number of survivors by plating on agar after nanophotometer assay or microtiter plate assay had confirmed the effectiveness of honey as antimicrobial agent against target bacteria; which confirmed that honey has the potency to inhibit pathogens even at low concentration.

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Two Major Medicinal Honeys Have Different Mechanisms of Bactericidal Activity

Cited by 235 publications

References 37 publications

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Colour Intensity, Polyphenol Content and Antibacterial Capacity of Unheated and Heat-Treated Sahara Honey

Potency of Honey as Antibacterial Agent Against Multiple Antibiotic Resistant Pathogens Evaluated by Different Methods

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