Two-Level Micro-to-Nanoscale Hierarchical TiO2 Nanolayers on Titanium Surface

Abstract

Joint replacement is being actively developed within modern orthopedics. One novel material providing fast implantation is bioactive coatings. The synthesis of targeted nanocoatings on metallic nanotitanium surface is reported in this paper. TiO2-based micro- and nanocoatings were produced by sol-gel synthesis using dip-coating technology with subsequent fast (shock) drying in hot plate mode at 400 °C. As a result of shock drying, the two-level hierarchical TiO2 nanolayer on the nanotitanium was obtained. This two-level hierarchy includes nanorelief of porous xerogel and microrelief of the micron-sized “defect” network (a crack network). The thickness of TiO2 nanolayers was controlled by repeating dip-coating process the necessary number of times after the first layer deposition. The state of the MS3T3-E1 osteoblast cell line (young cells that form bone tissue) on the two-level hierarchical surface has been studied. Particularly, adhesion character, adhesion time and morphology have been studied. The reported results may serve the starting point for the development of novel bioactive coatings for bone and teeth implants.

Bioactive Coating with Two-Layer Hierarchy of Relief Obtained by Sol-Gel Method with Shock Drying and Osteoblast Response of Its Structure

“…Using this approach, we fabricated a nanoporous layer with micron-sized defects. This article continues our previous work [21], in which we showed the effect of acceleration of osteoblast-like cell adhesion after this kind of modification. More detailed evaluation of the efficiency was based on a deep cytological analysis that included the study of the marker levels of the osteoblast-like cell differentiation, meaning the mineralization of the peri-implant area.…”

“…Thus, xerogel films were obtained. As we showed in our earlier work [21], this method leads to anatase.…”

“…We noted recently [21] that the samples with a developed relief demonstrate enhanced surface coverage by osteoblast-like cells and adhesion. In addition to this, we investigated the alkaline phosphatase activity and osteopontin expression that indicated the mineralization of the pre-implant area for short and long incubation periods.…”

Improved osseointegration properties of hierarchical microtopographic/nanotopographic coatings fabricated on titanium implants

“…In the current study, we applied the method of ML-ALD that provided the possibility to efficiently and precisely obtain the required surface microtopographic/nanotopographic parameters ( Figure 4; Table 3). The efficacy of this approach could be compared to the currently employed methods including anodic oxidation, plasma deposition, chemical vapor deposition, sol-gel synthesis, 43 thermal spray deposition, and electrostatic spray. 31,34 In the series of in vitro experiments, we clearly demonstrated that hierarchical microtopographic/ nanotopographic coatings significantly enhanced osteogenic cell differentiation of mouse MC3T3-E1 osteoblasts ( Figures 5 and 6).…”

A Feasible Strategy for Fabricating Surface Porous Network in Fe-Si Ribbons

“…These composites with excellent performance are promising for application in electrode/collector materials [ 16 , 17 ]. The transition metal oxides [ 18 , 19 , 20 ] are considered to be promising candidates for anode materials owing to their high electrochemical capacities. Many studies on iron oxides have also been performed with the aim of improving the battery performance of cathode materials [ 21 , 22 ] and removing anionic and aromatic dyes from wastewater [ 23 ].…”