Two Layered Scaffolds (Loofah/PLLA/Cellulose/Chitin) for Repair of Osteochondral Defect

Çeçen, Berivan; Kozacı, Leyla Didem; Yüksel, Münevver; Kara, Aylin; Ersoy, Nevin

doi:10.4172/2157-7552.1000210

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…Fibrin glue (FG), also called brin sealant, generally consists of two main components: brinogen and thrombin. Fibrin is an activated form of brinogen that is activated by the thrombin, and the activated brin glue has a certain viscosity, which is conducive to blood coagulation and wound closure 12,13 . Fibrin is composed of linked brinogens, they play important roles in coagulation, in ammation, brinolysis, tissue remodeling, cellular and matrix interactions, and wound healing.…”

Section: Introductionmentioning

confidence: 99%

The healing capacity and osteogenesis pattern of Demineralized Dentin Matrix (DDM)-Fibrin Glue (FG) compound

Bao

et al. 2023

Preprint

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Demineralized dentin matrix (DDM) is an osteoconductive and osteoinductive material, which was successfully performed in sinus floor augmentation and alveolar ridge augmentation in clinical application. It releases bone morphogenetic protein (BMP) and other growth factors which make DDM a suitable grafting material. However, the granular particle of DDM makes it difficult to anchor into the bone defect area. The aim of this study was to investigate biological effects as well as an osteoinductivity of the combination of DDM and Fibrin Glue (FG) at an optimal ratio on bone healing from critical bone defect in an animal model. Mouse osteoblastic cell line (MC3T3-E1) was co-cultured with various ratios of DDM and FG to examine their effects on osteoblast proliferation and differentiation indicated by alkaline phosphatase (ALP) activity, osteocalcin (OC) production and mineralized nodules formation. The optimal ratio was then chosen to further study with rabbit calvarial defective model which they were implanted with DDM or DDM-FG1 (1:0.1) and DDM-FG2 (1:0.5) compounds (w/v) or blank for 2, 4, 8 and 12weeks for investigating soft tissue and new bone regeneration. Micro-CT and histology analysis were used to evaluate the total grafting properties according to the different healing periods. The result from in vitro studies demonstrated that the ratio of 1:0.1 induced more ALP activity, mineralized nodules at the same time, 1: 0.5 (DDM-FG combined) induced more Osteocalcin (OC) at given days. In animal model, the 3D new bone volume in all DDM-FG treatment were significantly greater than blank group at 2, 4, 8 and 12 weeks. Furthermore, the new bone volume was greater in DDM-FG2 when compared to other groups at early week of the healing period. In histological analysis, numbers of osteoblasts were formed as a cluster adjacent to the DDM particles where we could observe a newly formed bone in all groups suggesting an osteoinductive property of DDM. Moreover, the greater new collagen synthesis observed at 4 weeks suggested that the early bone healing was induced in a group treated with DDM-FG2. This study demonstrates that at optimal ratio of DDM-FG compound enhances the osteogenic activities and bone regeneration.

show abstract

Section: Introductionmentioning

confidence: 99%

The healing capacity and osteogenesis pattern of Demineralized Dentin Matrix (DDM)-Fibrin Glue (FG) compound

Bao

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Fibrin glue (FG), also called fibrin sealant, generally consists of two main components: fibrinogen and thrombin. Fibrin is an activated form of fibrinogen that is activated by the thrombin, and the activated fibrin glue has a certain viscosity, which is conducive to blood coagulation and wound closure 12 , 13 . Fibrin is composed of linked fibrinogens, they play important roles in coagulation, inflammation, fibrinolysis, tissue remodeling, cellular and matrix interactions, and wound healing.…”

Section: Introductionmentioning

confidence: 99%

The healing capacity and osteogenesis pattern of demineralized dentin matrix (DDM)-fibrin glue (FG) compound

Bao,

Fu,

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Demineralized dentin matrix (DDM) is an osteoconductive and osteoinductive material that has been successfully used in sinus floor augmentation and alveolar ridge augmentation in clinical applications. It releases bone morphogenetic proteins (BMPs) and other growth factors, making DDM a suitable grafting material. However, the granular particle of DDM makes it difficult to anchor into the bone defect area. The aim of this study was to investigate the biological effects and osteoinductivity of the combination of DDM and Fibrin Glue (FG) at an optimal ratio on bone healing from a critical bone defect in an animal model. The mouse osteoblastic cell line (MC3T3-E1) was co-cultured with various ratios of DDM and FG to examine their effects on osteoblast proliferation and differentiation, as indicated by alkaline phosphatase (ALP) activity, osteocalcin (OC) production and mineralized nodules formation. The optimal ratio was then chosen for further study with a rabbit calvarial defective model, in which they were implanted with DDM or DDM-FG1 (1 g: 0.1 ml) and DDM-FG2 (1 g: 0.5 ml) compounds, or left blank for 2, 4, 8 and 12 weeks to investigate soft tissue and new bone regeneration. Micro-CT and histology analysis were used to evaluate the total grafting properties according to the different healing periods. The result from in vitro studies demonstrated that the ratio of 1:0.1 induced more ALP activity and mineralized nodules, while the ratio of 1: 0.5 (DDM-FG combined) induced more osteocalcin (OC) at specific time points. In the animal model, the 3D new bone volume in all DDM-FG treatment groups was significantly greater than that in the blank group at 2, 4, 8 and 12 weeks. Furthermore, the new bone volume was greater in DDM-FG2 when compared to the other groups during the early weeks of the healing period. In histological analysis, clusters of osteoblasts were formed adjacent to the DDM particles, and newly formed bone was observed in all groups, suggesting an osteoinductive property of DDM. Moreover, the greater new collagen synthesis observed at 4 weeks suggested that early bone healing was induced in the DDM-FG2 group. This study demonstrated that at an optimal ratio, the DDM-FG compound enhances osteogenic activities and bone regeneration.

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Using loofah reinforced chitosan-collagen hydrogel based scaffolds in-vitro and in-vivo; healing in cartilage tissue defects

Baysan,

Gunes,

Turemis

et al. 2023

Materialia

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Two Layered Scaffolds (Loofah/PLLA/Cellulose/Chitin) for Repair of Osteochondral Defect

Cited by 4 publications

References 24 publications

The healing capacity and osteogenesis pattern of Demineralized Dentin Matrix (DDM)-Fibrin Glue (FG) compound

The healing capacity and osteogenesis pattern of Demineralized Dentin Matrix (DDM)-Fibrin Glue (FG) compound

The healing capacity and osteogenesis pattern of demineralized dentin matrix (DDM)-fibrin glue (FG) compound

Using loofah reinforced chitosan-collagen hydrogel based scaffolds in-vitro and in-vivo; healing in cartilage tissue defects

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