Using loofah reinforced chitosan-collagen hydrogel based scaffolds in-vitro and in-vivo; healing in cartilage tissue defects

Baysan, Gizem; Gunes, Oylum Colpankan; Turemis, Cihangir; Akokay Yilmaz, Pınar; Husemoglu, R. Bugra; Kara Ozenler, Aylin; Perpelek, Merve; Albayrak, Aylin Ziylan; Havitcioglu, Hasan; Cecen, Berivan

doi:10.1016/j.mtla.2023.101881

Cited by 4 publications

(1 citation statement)

References 41 publications

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“…On the other hand, as the nanofibrous structure, PHBV was used as it has good spinnability under wet-electrospinning conditions as well as it is biocompatible and biodegradable [34]. In our previous studies, wet electrospun PHBV nanofibers were used together with the macro-fibrous loofah mat in the design of scaffolds for both meniscus and osteochondral tissue regeneration [13,32]. These fibrous structures not only reinforced the hydrogels but also, they became favorable sites for cell attachment.…”

Section: Fabrication Of Lcpg Lcpg-h and Lcpg-e Scaffoldsmentioning

confidence: 99%

Loofah and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nano-fiber-reinforced chitosan hydrogel composite scaffolds with elderberry (Sambucus nigra) and hawthorn (Crataegus oxyacantha) extracts as additives for osteochondral tissue engineering applications

Baysan,

Yilmaz,

Albayrak

et al. 2024

Polym. Bull.

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In recent years, people have had more expectations from the developed technology in medicine, especially in the field of orthopedics and traumatology. Tissue engineers are interested in techniques that benefit from patients’ cells and biomaterials, instead of prostheses and implants. On the other hand, researchers have begun to use various medicinal plants for regeneration and anti-cancer studies. In the present study, we aimed to produce cartilage and bone inductive scaffolds for osteochondral tissue engineering applications with the addition of hawthorn or elderberry extracts. Firstly, wet electro-spun poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) fibers were integrated with a loofah mat. Then, they were impregnated into chitosan solution with/without hawthorn or elderberry extract. Composite hydrogel scaffolds were obtained by cross-linking with 0.3% (w/v) genipin. Fabricated scaffolds had more than 90% porosity and showed swelling capacity in the range of 1500–2200%. Based on the in vitro biocompatibility analyses using mesenchymal stem cells (MSCs), all the fabricated scaffolds were found to be biocompatible by WST-1, ALP activity, and GAG content analysis. Also, histological/immunohistochemical analyses showed that hawthorn and elderberry extract addition increased MSCs proliferation and collagen type I and II positivity. Consequently, all the scaffolds showed promising features for osteochondral tissue engineering applications. Graphical abstract

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Section: Fabrication Of Lcpg Lcpg-h and Lcpg-e Scaffoldsmentioning

confidence: 99%

Loofah and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nano-fiber-reinforced chitosan hydrogel composite scaffolds with elderberry (Sambucus nigra) and hawthorn (Crataegus oxyacantha) extracts as additives for osteochondral tissue engineering applications

Baysan,

Yilmaz,

Albayrak

et al. 2024

Polym. Bull.

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A comprehensive review on hydrogel-based bio-ink development for tissue engineering scaffolds using 3D printing

Gogoi,

Kumar,

Singh

2024

Annals of 3D Printed Medicine

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In Vitro and In Vivo Evaluations of Loofah Micro- and PHBV Nano-Fiber Integrated Hydrogel Scaffolds for Meniscus Regeneration

Baysan,

Akdogan,

Gunes

et al. 2024

Preprint

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Meniscus is a tissue that has vital properties for knee stabilization, shock absorption, axial load distribution, joint lubrication, and nutrition of articular cartilage. This study aims to produce loofah micro- and poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nano-fiber integrated collagen and chitosan polymer-based composite hydrogel scaffolds crosslinked with three different concentrations of genipin (0.1, 0.3, and 0.5%) for meniscus regeneration. The scaffold crosslinked with 0.3% genipin, which exhibites the highest compression strength with high water absorption and damping capacities, is chosen as the optimal scaffold for further in vitro and in vivo analyses. In vitro biocompatibility studies are conducted by using rabbit bone marrow-derived mesenchymal stem cells. Accordingly, the composite hydrogel scaffold is found to be non-toxic, and capable of promoting cell adhesion and proliferation as well as collagen immunopositive, especially for type II collagen. In vivo analysis is performed by using 24 adult male New Zealand rabbits in three groups (empty defect, cell-free and cell-laden implanted scaffolds) with a standardized meniscus regeneration model. In conclusion, the cell-laden scaffold implanted group shows better meniscal healing based on the post-implantation biomechanical, histological, immunohistochemical, and Micro-CT evaluations.

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Using loofah reinforced chitosan-collagen hydrogel based scaffolds in-vitro and in-vivo; healing in cartilage tissue defects

Cited by 4 publications

References 41 publications

Loofah and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nano-fiber-reinforced chitosan hydrogel composite scaffolds with elderberry (Sambucus nigra) and hawthorn (Crataegus oxyacantha) extracts as additives for osteochondral tissue engineering applications

Loofah and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nano-fiber-reinforced chitosan hydrogel composite scaffolds with elderberry (Sambucus nigra) and hawthorn (Crataegus oxyacantha) extracts as additives for osteochondral tissue engineering applications

A comprehensive review on hydrogel-based bio-ink development for tissue engineering scaffolds using 3D printing

In Vitro and In Vivo Evaluations of Loofah Micro- and PHBV Nano-Fiber Integrated Hydrogel Scaffolds for Meniscus Regeneration

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