Two jasmonate-responsive factors, TcERF12 and TcERF15, respectively act as repressor and activator of tasy gene of taxol biosynthesis in Taxus chinensis

Zhang, Meng; Li, Shutao; Lin, Na; Chen, Qingpu; Xu, Xiangping; Yu, Liang; Fu, Chunhua

doi:10.1007/s11103-015-0382-2

Cited by 55 publications

(66 citation statements)

References 57 publications

(63 reference statements)

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“…Our results seems to support the suggestion by Lenka et al (2012) that the taxane biosynthesis capacity is controlled rather by genes induced early post-MJ addition, but genes not only involved in the biosynthesis itself but engaged in the total metabolism. Moreover, in T. chinensis two jasmonate-responsive elements acting in the opposite way were discovered (Zhang et al 2015). Among them the TcERF12 acted as the repressor, while TcRF15 as an activator of TXS gene expression.…”

Section: Resultsmentioning

confidence: 99%

Comparison of elicitor-based effects on metabolic responses of Taxus × media hairy roots in perfluorodecalin-supported two-phase culture system

et al. 2018

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Key messageTwo lines of Taxus × media hairy roots harbouring or not the TXS transgene demonstrated diverse gene expression and taxane yield during cultivation in PFD-supported two liquid-phase culture system.AbstractTwo lines of Taxus × media hairy roots were subjected to single or twice-repeated supplementation with methyl jasmonate, sodium nitroprusside, l-phenylalanine, and sucrose feeding. One line harboured transgene of taxadiene synthase (ATMA), while the second (KT) did not. Both hairy root lines were cultured in two-phase culture systems containing perfluorodecalin (PFD) in aerated or degassed form. The relationship between TXS (taxadiene synthase), BAPT (baccatin III: 3-amino, 3-phenylpropanoyltransferase), and DBTNBT (3′-N-debenzoyl-2-deoxytaxol-N-benzoyltransferase) genes and taxane production was analysed. The ATMA and KT lines differed in their potential for taxane accumulation, secretion, and taxane profile. In ATMA biomass, both paclitaxel and baccatin III were detected, while in KT roots only paclitaxel. The most suitable conditions for taxane production for ATMA roots were found in single-elicited supported with PFD-degassed cultures (2 473.29 ± 263.85 µg/g DW), whereas in KT roots in single-elicited cultures with PFD-aerated (470.08 ± 25.15 µg/g DW). The extracellular levels of paclitaxel never exceeded 10% for ATMA roots, while for KT increased up to 76%. The gene expression profile was determined in single-elicited cultures supported with PFD-degassed, where in ATMA roots, the highest taxane yield was obtained, while in KT the lowest one. The gene expression pattern in both investigated root lines differed substantially what resulted in taxane yield characterized particular lines. The highest co-expression of TXS, BAPT and DBTNBT genes noted for ATMA roots harvested 48 h after elicitation corresponded with their higher ability for taxane production in comparison with the effects observed for KT roots.Electronic supplementary materialThe online version of this article (10.1007/s00299-018-2351-0) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Comparison of elicitor-based effects on metabolic responses of Taxus × media hairy roots in perfluorodecalin-supported two-phase culture system

et al. 2018

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“…Taxol is the anticancer compoundand has also been functionally correlated with plant defense against oomycete fungi Phytophthora capsici [61]. Two ERF TFs, TcERF12 and TcERF15 were identified to act as the negative or positive regulator of taxol biosynthesis, respectively [62]. Both of TcERF12 and TcERF15 were bound to the cis-element GCC-box on the tasy gene promoter to regulate taxol biosynthesis.…”

Section: Ap2/erf Tfsmentioning

confidence: 99%

Transcriptional Factors Regulate Plant Stress Responses Through Mediating Secondary Metabolism

Meraj

Raza

et al. 2020

Genes

167

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Plants are adapted to sense numerous stress stimuli and mount efficient defense responses by directing intricate signaling pathways. They respond to undesirable circumstances to produce stress-inducible phytochemicals that play indispensable roles in plant immunity. Extensive studies have been made to elucidate the underpinnings of defensive molecular mechanisms in various plant species. Transcriptional factors (TFs) are involved in plant defense regulations through acting as mediators by perceiving stress signals and directing downstream defense gene expression. The cross interactions of TFs and stress signaling crosstalk are decisive in determining accumulation of defense metabolites. Here, we collected the major TFs that are efficient in stress responses through regulating secondary metabolism for the direct cessation of stress factors. We focused on six major TF families including AP2/ERF, WRKY, bHLH, bZIP, MYB, and NAC. This review is the compilation of studies where researches were conducted to explore the roles of TFs in stress responses and the contribution of secondary metabolites in combating stress influences. Modulation of these TFs at transcriptional and post-transcriptional levels can facilitate molecular breeding and genetic improvement of crop plants regarding stress sensitivity and response through production of defensive compounds.

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“…The roles of these TFs were analyzed here on the basis of studies on several known Taxus TFs [33][34][35][36]. TcERF15, TcMYC2a, TcJAMYC1/2/4, and TcWRKY1/ 8/20/26/47/52 were highly correlated with taxol biosynthesis genes with Pearson coefficients greater than 0.9 (Fig.…”

Section: Tfs Are Important Targets Of Mirnasmentioning

confidence: 99%

“…These results are highly consistent with results from functional studies. TcMYC2a from Taxus chinensis and TcJAMYC1/2/4 from Taxus cuspidata function as positive and negative regulators in taxol biosynthesis, respectively [34].…”

Section: Tfs Are Important Targets Of Mirnasmentioning

confidence: 99%

Transcriptional reprogramming strategies and miRNA-mediated regulation networks of Taxus media induced into callus cells from tissues

et al. 2020

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Background: Taxus cells are a potential sustainable and environment-friendly source of taxol, but they have low survival ratios and slow grow rates. Despite these limitations, Taxus callus cells induced through 6 months of culture contain more taxol than their parent tissues. In this work, we utilized 6-month-old Taxus media calli to investigate their regulatory mechanisms of taxol biosynthesis by applying multiomics technologies. Our results provide insights into the adaptation strategies of T. media by transcriptional reprogramming when induced into calli from parent tissues.Results: Seven out of 12 known taxol, most of flavonoid and phenylpropanoid biosynthesis genes were significantly upregulated in callus cells relative to that in the parent tissue, thus indicating that secondary metabolism is significantly strengthened. The expression of genes involved in pathways metabolizing biological materials, such as amino acids and sugars, also dramatically increased because all nutrients are supplied from the medium. The expression level of 94.1% genes involved in photosynthesis significantly decreased. These results reveal that callus cells undergo transcriptional reprogramming and transition into heterotrophs. Interestingly, common defense and immune activities, such as "plant-pathogen interaction" and salicylic acid-and jasmonic acidsignaling transduction, were repressed in calli. Thus, it's an intelligent adaption strategy to use secondary metabolites as a cost-effective defense system. MiRNA-and degradome-sequencing results showed the involvement of a precise regulatory network in the miRNA-mediated transcriptional reprogramming of calli. MiRNAs act as direct regulators to enhance the metabolism of biological substances and repress defense activities. Given that only 17 genes of secondary metabolite biosynthesis were effectively regulated, miRNAs are likely to play intermediate roles in the biosynthesis of secondary metabolites by regulating transcriptional factors (TFs), such as ERF, WRKY, and SPL.

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Two jasmonate-responsive factors, TcERF12 and TcERF15, respectively act as repressor and activator of tasy gene of taxol biosynthesis in Taxus chinensis

Cited by 55 publications

References 57 publications

Comparison of elicitor-based effects on metabolic responses of Taxus × media hairy roots in perfluorodecalin-supported two-phase culture system

Comparison of elicitor-based effects on metabolic responses of Taxus × media hairy roots in perfluorodecalin-supported two-phase culture system

Transcriptional Factors Regulate Plant Stress Responses Through Mediating Secondary Metabolism

Transcriptional reprogramming strategies and miRNA-mediated regulation networks of Taxus media induced into callus cells from tissues

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