Two interferon sensitive steps in the replication cycle of rous sarcoma virus

Zens, Wolfgang; Degen, Hans-Joachim; Barnekow, Angelika; Gelderblom, Hans R.; Jungwirth, C.

doi:10.1016/0042-6822(89)90623-5

Cited by 4 publications

(1 citation statement)

References 35 publications

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“…In the avian retrovirus system, both early effects at or before proviral DNA synthesis and late effects on virus maturation have been noted (20,29). Zens et al (28) have recently shown that uncoating and reverse transcription are inhibited in de novo infection of chicken embryo fibroblasts by Rous sarcoma virus.…”

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Inhibition of human immunodeficiency virus type 1 morphogenesis in T cells by alpha interferon

Smith

Thresher

Pagano

1991

Antimicrob Agents Chemother

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Some murine retroviruses exhibit altered release of virus when cells are treated with alpha interferon (IFN-oa), resulting in the accumulation of intraceUlular virions in cytoplasmic vacuoles. In studies of the inhibitory effect of IFN-ac (Welferon) on acute human immunodeficiency virus type 1 infection of human T-cell lines, we found that in C3 cells, the 50% effective concentration was 9 U/ml and the 90% effective concentration was 310 U/ml. There was no apparent accumulation of intracelular particles detected by p24 antigen levels or by processing the cells for electron microscopy. ExtraceUlular reverse transcriptase activity and p24 levels decreased in parallel with increasing IFN, whereas the intracellular viral proteins decreased only slightly. By electron microscopy, cells treated with higher concentrations of IFN (512 U/mi) disclosed very few particles budding into extracellular spaces; no intracellular particles could be seen, despite nearly normal levels of intracellular viral protein detected by the p24 antigen assay and correct processing detected by Western blot (immunoblot) analysis. Thus in human immunodeficiency virus-infected cells, the major block produced by IFN-a appeared to be late in the viral cycle at the morphogenesis stage of virion production. Chronically infected Jurkat cells treated with IFN appeared to be inhibited in growth rate, as virus production decreased proportionally with cell number.

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confidence: 99%