Two Expansin Genes, AtEXPA4 and AtEXPB5, Are Redundantly Required for Pollen Tube Growth and AtEXPA4 Is Involved in Primary Root Elongation in Arabidopsis thaliana

Liu, Weimiao; Xu, Liai; Lin, Hui; Cao, Jiashu

doi:10.3390/genes12020249

Cited by 43 publications

(27 citation statements)

References 57 publications

(70 reference statements)

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“…Previous studies have shown that genes encoding expansin protein are closely related to plant wall development and fertility (Liu et al ., 2021). Here, to investigate whether TaEXPB5 is related to pollen wall development and male fertility as previously described, we conducted the following experiments, including observing the external morphology, outer epidermis, and microspores of Tns anthers by SEM after TaEXPB5 silencing (Fig.…”

Section: Resultsmentioning

confidence: 99%

TaEXPB5 is responsible for male fertility in thermo-sensitive male-sterility wheat with Aegilops kotschyi cytoplasm

Geng

Wang

et al. 2021

Preprint

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Thermo-sensitive male sterility is of vital importance to heterosis, or hybrid vigor in crop production and hybrid breeding. Therefore, it is meaningful to study the function of the genes related to pollen development and male sterility, which is still not fully understand currently. Here, we conducted comparative analyses to screen fertility related genes using RNA-seq, iTRAQ, and PRM-based assay. A gene encoding expansin protein in wheat, TaEXPB5, was isolated in KTM3315A, which was in the cell wall and preferentially upregulated expression in the fertility anthers. The silencing of TaEXPB5 displayed pollen abortion, the declination or sterility of fertility. Further, cytological investigation indicated that the silencing of TaEXPB5 induced the early degradation of tapetum and abnormal development of pollen wall. These results revealed that the silencing of TaEXPB5 could eliminate the effects of temperature on male fertility, and resulting in functional loss of fertility conversion, which implied that TaEXPB5 may be essential for anther or pollen development and male fertility of KTM3315A. These findings provide a novel insight into molecular mechanism of fertility conversion for thermo-sensitive cytoplasmic male-sterility wheat, and contribute to the molecular breeding of hybrid wheat in the future.

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Section: Resultsmentioning

confidence: 99%

TaEXPB5 is responsible for male fertility in thermo-sensitive male-sterility wheat with Aegilops kotschyi cytoplasm

Geng

Wang

et al. 2021

Preprint

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“…Among the MYB93-regulated genes, several expansin genes were detected. Expansins are known as the cell wall loosening enzymes (McQueen-Mason and Cosgrove, 1994; Cosgrove, 2005) and play variety roles for plant development such as hypocotyl elongation, root development, and pollen tube growth (Son et al, 2012; Ma et al, 2013; Lee and Kim, 2013; Lee et al, 2013; Liu et al, 2021). The detailed involvement of EXP17 in LR development has been reported for Arabidopsis (Lee and Kim, 2013, Lee et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

A very long chain fatty acid responsive transcription factor, MYB93, regulates lateral root development in Arabidopsis

Uemura

Kimura

Ohta

et al. 2022

Preprint

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Lateral roots (LRs) are critical to rhizosphere development in plants. Although the molecular mechanisms by which auxin regulates LR development has been studied extensively, many additional regulatory systems are thought to be involved. Based on the expression analysis of LTPG1 and 2, we found that they were specifically expressed at the developing LR primordium (LRP), and the number of LRs were reduced in these mutants. Because LTPG is a protein that transports Very Long Chain Fatty Acids (VLCFAs), we hypothesized that VLCFAs regulated LR development. We revealed that late LRP development was stunted when VLCFA levels were reduced in the synthetic mutant, kcs1-5. In this study, we established a method to analyze the LRP development stages with high temporal resolution using a deep neural network. We identified a VLCFA responsible transcription factor, MYB93, from transcriptome analysis of kcs1-5. Interestingly, MYB93 showed a carbon chain length-specific expression response after treatment of VLCFA. Furthermore, myb93 transcriptome analysis suggested that MYB93 regulated the expression of cell wall organization genes. Our results indicated that VLCFA is a regulator of LRP development through transcription factor-mediated regulation of gene expression.

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“…For example, AT4G01700-encoding protein belongs to Chitinase family which is related to cellulose biosynthesis and cell wall remodeling [128]. The EXPA proteins are responsible for controlling cell wall extension and developmental processes, including cell dissociation and separation [129][130][131][132]. LTP1 and LTP2 belong to a family of lipid transfer proteins, and LTP2 plays a role in maintaining the integrity of the cuticle-cell wall interface [133].…”

Section: Discussionmentioning

confidence: 99%

AtSYP71 Is Important for Plant Cell Wall Biosynthesis and Stress Adaptation.

Kou

Zhang

Zhao

et al. 2021

Preprint

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Background: SYP71, the plant-specific Qc-SNARE protein, is reported to regulate vesicle trafficking. SYP71 is localized on the ER, endosome, plasma membrane and cell plate, suggesting its multiple functions. Lotus SYP71 is essential for symbiotic nitrogen fixation in nodules. AtSYP71, GmSYP71 and OsSYP71 are implicated in plant resistance to pathogenesis. To date, SYP71 regulatory role on plant development remain unclear.Results: AtSYP71-knockout mutant atsyp71-4 was lethal at early development stage. Early development of AtSYP71-knockdown mutant atsyp71-2 was delayed, and stress response was also affected. Confocal images revealed that protein secretion was blocked in atsyp71-2. Transcriptomic analysis indicated that metabolism, response to environmental stimuli pathways and apoplast components were influenced in atsyp71-2. Moreover, the contents of lignin, cellulose and flavonoids as well as cell wall structures were also altered.Conclusion: Our findings suggested that AtSYP71 is essential for plant development. AtSYP71 probably regulates plant development, metabolism and environmental adaptation by affecting cell wall homeostasis via mediating secretion of materials and regulators required for cell wall biosynthesis and dynamics.

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Two Expansin Genes, AtEXPA4 and AtEXPB5, Are Redundantly Required for Pollen Tube Growth and AtEXPA4 Is Involved in Primary Root Elongation in Arabidopsis thaliana

Cited by 43 publications

References 57 publications

TaEXPB5 is responsible for male fertility in thermo-sensitive male-sterility wheat with Aegilops kotschyi cytoplasm

TaEXPB5 is responsible for male fertility in thermo-sensitive male-sterility wheat with Aegilops kotschyi cytoplasm

A very long chain fatty acid responsive transcription factor, MYB93, regulates lateral root development in Arabidopsis

AtSYP71 Is Important for Plant Cell Wall Biosynthesis and Stress Adaptation.

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