A very long chain fatty acid responsive transcription factor, MYB93, regulates lateral root development in Arabidopsis

Uemura, Yoshio; Kimura, Saori; Ohta, Tomomichi; Suzuki, Takamasa; Mase, Kosuke; Kato, Hiroyuki; Sakaoka, Satomi; Komine, Yuki; Shimizu, Motoyuki; Morikami, Atsushi; Tsukagoshi, Hironaka

doi:10.1101/2022.05.24.493307

Cited by 2 publications

(7 citation statements)

References 56 publications

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“…For YFP-cMYB50, YFP-cPMEI8, YFP-cUPB1 and CFP-cUPB1 cloning, the cMYB50, cPMEI8, and cUPB1 cDNA regions were amplified using forward and reverse primers containing the BamHI site just before the MYB50, PMEI8, or UPB1 termination codon. The cMYB50-Bam HI, cPMEI8-Bam HI, and cUPB1-Bam HI fragments were cloned into Aor51H1, and the Bam HI sites of YFP-Aor51H1-Bam HI-pDONR201 plasmids [16] and CFP-Aor51H1-Bam HI-pDONR201 plasmids [20], respectively.…”

Section: Plasmid Construction and Plant Transformationmentioning

confidence: 99%

AtMYB50 regulates root cell elongation by upregulatingPECTIN METHYLESTERASE INHIBITOR 8inArabidopsis thaliana

Mase

Mizuno

Nakamichi

et al. 2023

Preprint

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Plant root development is regulated by several signal transduction pathways. Among them, plant phytohormones, like auxin and cytokinin, are well characterized for their molecular mechanisms of action. Reactive oxygen species (ROS) play important roles as signaling molecules in controlling root development. Under these signal transduction pathways, the gene regulatory network, which is controlled by transcription factors, is the key to regulating root growth. We have previously reported an important transcription factor, UP BEAT1 (UPB1), that regulates ROS homeostasis at the root tip, further controlling the transition from cell proliferation to differentiation. Although UPB1 directly regulates the expression of several peroxidases that control ROS homeostasis, UPB1 still targets genes other than peroxidases. This indicates that UPB1 may regulate root growth through different ROS signals. Here, we investigated the function of the transcription factor MYB50, a direct target of UPB1, in Arabidopsis thaliana. We then examined whether UPB1 regulates MYB50 expression in the roots using an induction expression system and imaging of multiple fluorescent proteins. We also performed RNA-Seq analysis using MYB50 estradiol induction lines and ChIP-seq analysis to identify the MYB50 regulatory gene network. Integrating these analyses with UPB1 regulatory network revealed that MYB50 regulates the expression of PECTIN METHYLESTERASE INHIBITOR 8 (PMEI8). These data suggest that MYB50 is a new root growth regulator under the UPB1 gene regulatory network, which differs from the control of ROS homeostasis. Our study presents a model including a new transcriptional network under MYB50 into UPB1 regulatory root growth system and will provide novel insights into the cell elongation controlled by pectin modification.

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Section: Plasmid Construction and Plant Transformationmentioning

confidence: 99%

AtMYB50 regulates root cell elongation by upregulatingPECTIN METHYLESTERASE INHIBITOR 8inArabidopsis thaliana

Mase

Mizuno

Nakamichi

et al. 2023

Preprint

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“…Recently, we reported that very long-chain fatty acids (VLCFAs) are involved in LR development through the regulation of the expression of the transcription factor MYB93 (Uemura et al, 2023). In addition to MYB93, ATML1, which has a START domain (a lipid-binding region), reportedly binds to very long-chain ceramides and plays a key role in epidermal differentiation (Abe et al, 2003; Lu et al, 1996; Sessions et al, 1999).…”

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confidence: 99%

“…The VLCFA levels decreased in kcs1-5 mutants, indicating that MYB93 is a novel transcription factor whose expression responds to VLCFA levels. MYB93 expression shows a specific response to fatty acid carbon chain length, with no response at C18 but a response to C20–C24 VLCFAs (Uemura et al, 2023). Moreover, genetic analysis has revealed that MYB93 is involved in the late stages of LR development by regulating the expression of several cell wall remodelling genes, such as expansins (Uemura et al, 2023).…”

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confidence: 99%

“…MYB93 expression shows a specific response to fatty acid carbon chain length, with no response at C18 but a response to C20–C24 VLCFAs (Uemura et al, 2023). Moreover, genetic analysis has revealed that MYB93 is involved in the late stages of LR development by regulating the expression of several cell wall remodelling genes, such as expansins (Uemura et al, 2023).

Figure 1. Comparison of conventional lateral root primordia (LRP) stage analysis methods (gravistimulation method) with a time-lapse imaging followed by a deep neural network (DNN) analysis method.…”

mentioning

confidence: 99%

“…Significant difference from Col-0 was determined using the Welch’s t -test (** P = 0.004). The data were retrieved from Uemura et al (2023). (b) Schematic model of the gravistimulation method.…”

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Quantitative analysis of lateral root development with time-lapse imaging and deep neural network

Uemura,

Tsukagoshi

2024

Quant Plant Bio.

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During lateral root (LR) development, morphological alteration of the developing single LR primordium occurs continuously. Precise observation of this continuous alteration is important for understanding the mechanism involved in single LR development. Recently, we reported that very long-chain fatty acids are important signalling molecules that regulate LR development. In the study, we developed an efficient method to quantify the transition of single LR developmental stages using time-lapse imaging followed by a deep neural network (DNN) analysis. In this ‘insight’ paper, we discuss our DNN method and the importance of time-lapse imaging in studies on plant development. Integrating DNN analysis and imaging is a powerful technique for the quantification of the timing of the transition of organ morphology; it can become an important method to elucidate spatiotemporal molecular mechanisms in plant development.

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A very long chain fatty acid responsive transcription factor, MYB93, regulates lateral root development in Arabidopsis

Cited by 2 publications

References 56 publications

AtMYB50 regulates root cell elongation by upregulatingPECTIN METHYLESTERASE INHIBITOR 8inArabidopsis thaliana

AtMYB50 regulates root cell elongation by upregulatingPECTIN METHYLESTERASE INHIBITOR 8inArabidopsis thaliana

Quantitative analysis of lateral root development with time-lapse imaging and deep neural network

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