Two-dye based arrayed primer extension for simultaneous multigene detection in lipid metabolism

Toehold-Mediated Strand Displacement Reaction for Dual-Signal Electrochemical Assay of Apolipoprotein E Genotyping

“…The apoE genotyping involves the discrimination of the single mismatched sequences at codons 112 and 158 as well as the sequence detection. The methods for the discrimination of the apoE genotypes include the polymerase chain reaction (PCR) based on TaqMan probe recognition, primer extension, and gene amplification and cleavage . The TaqMan probe-based PCR uses a TaqMan probe as the polymorphic allele, which is recognized by the site-specific PCR primer .…”

“…The TaqMan probe-based PCR uses a TaqMan probe as the polymorphic allele, which is recognized by the site-specific PCR primer . The primer extension and gene cleavage involve enzyme recognition and digestion of the mismatched sites, respectively. , Though commonly used, these methods usually involve multiple steps and longer assay time. Thus, the development of simple, convenient, and highly specific methods for apoE genotyping is still challenging.…”

Dual-Channel Surface Plasmon Resonance for Quantification of ApoE Gene and Genotype Discrimination in Unamplified Genomic DNA Extracts

“…8 These methods usually involve tedious assay procedures, longer assay time, and highly trained operators, which limits their practical genotyping applications. Microarray offers multiplexed and parallel identification of apoE genotyping 7,9 but usually suffers from lower sensitivity and cross-reactivity. 10,11 Electrochemical methods are simple, rapid, and sensitive 12 and have been widely used in apoE genotyping.…”

“…Typically, traditional apoE genotyping protocols rely on hybridization-based genetic amplification, such as allele-specific polymerase chain reaction (PCR), , primer extension, and gene amplification and cleavage . These methods usually involve tedious assay procedures, longer assay time, and highly trained operators, which limits their practical genotyping applications.…”

“…These methods usually involve tedious assay procedures, longer assay time, and highly trained operators, which limits their practical genotyping applications. Microarray offers multiplexed and parallel identification of apoE genotyping , but usually suffers from lower sensitivity and cross-reactivity. , Electrochemical methods are simple, rapid, and sensitive and have been widely used in apoE genotyping. − Based on the modified Randles’ equivalent circuit, discrimination of the single-nucleotide mismatch in apoE alleles has been performed by comparing the difference in the charge transfer resistance before and after Ni 2+ addition . DNA electrochemical biosensors have been developed for the detection of apoE genotypes in PCR-amplified DNA extracted from human blood .…”