Two distinct adhesion systems are responsible for EDTA-sensitive adhesion in Dictyostelium discoideum

Fontana, Donna R.

doi:10.1111/j.1432-0436.1993.tb00702.x

Cited by 21 publications

(9 citation statements)

References 38 publications

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“…There are four adhesion systems in D. discoideum , three of which are well-characterized [22]. EDTA-sensitive, Ca 2+ -dependent adhesion sites are mediated by the protein DdCAD-1, which is encoded by the gene cadA , while the two EDTA-insensitive systems include the gp80 protein, encoded by csaA, and gp150 encoded by tgrC1 [23–26]. The importance of the latter two adhesion systems in social evolutionary analyses in D. discoideum has been widely recognized, as csaA is the first reported single green-beard gene, and tgrC1 is known to mediate kin discrimination in this social microbe [12, 13, 27].…”

Section: Resultsmentioning

confidence: 99%

Sociogenomics of self vs. non-self cooperation during development of Dictyostelium discoideum

Buttery

Thompson

et al. 2014

BMC Genomics

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BackgroundDictyostelium discoideum, a microbial model for social evolution, is known to distinguish self from non-self and show genotype-dependent behavior during chimeric development. Aside from a small number of cell-cell recognition genes, however, little is known about the genetic basis of self/non-self recognition in this species. Based on the key hypothesis that there should be differential expression of genes if D. discoideum cells were interacting with non-clone mates, we performed transcriptomic profiling study in this species during clonal vs. chimeric development. The transcriptomic profiles of D. discoideum cells in clones vs. different chimeras were compared at five different developmental stages using a customized microarray. Effects of chimerism on global transcriptional patterns associated with social interactions were observed.ResultsWe find 1,759 genes significantly different between chimera and clone, 1,144 genes associated significant strain differences, and 6,586 genes developmentally regulated over time. Principal component analysis showed a small amount of the transcriptional variance to chimerism-related factors (Chimerism: 0.18%, Chimerism × Timepoint: 0.03%). There are 162 genes specifically regulated under chimeric development, with continuous small differences between chimera vs. clone over development. Almost 60% of chimera-associated differential genes were differentially expressed at the 4 h aggregate stage, which corresponds to the initial transition of D. discoideum from solitary life to a multicellular phase.ConclusionsA relatively small proportion of over-all variation in gene expression is explained by differences between chimeric and clonal development. The relatively small modifications in gene expression associated with chimerism is compatible with the high level of cooperation observed among different strains of D. discoideum; cells of distinct genetic backgrounds will co-aggregate indiscriminately and co-develop into fruiting bodies. Chimeric development may involve re-programming of the transcriptome through small modifications of the developmental genetic network, which may also indicate that response to social interaction involves many genes with individually small transcriptional effect.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-616) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Sociogenomics of self vs. non-self cooperation during development of Dictyostelium discoideum

Buttery

Thompson

et al. 2014

BMC Genomics

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“…Six-hour-starved cells were prepared by washing non-starved cells twice in phosphate buffer (10 mM KH 2 PO 4 , 10 mM K 2 HPO 4 , pH 6.8), re-suspending them in phosphate buffer at 110 7 -210 7 cells/ml and shaken at 120 rpm for 6 h. To record the multi-cellular development of cells, nonstarved cells were washed twice in phosphate buffer and plated onto either phosphate-buffered or H 2 O 1 % non-nutrient agar at 510 5 Measurement of cell-cell adhesion. Non-starved and 6-h-starved cells were washed once in phosphate buffer, thoroughly pipetted and re-suspended in phosphate buffer at a cell density of 110 7 cells/ml.…”

Section: Methodsmentioning

confidence: 99%

“…At least four different cell-cell adhesion systems are employed by Dictyostelium cells after the onset of starvation. Two of these systems appear shortly after and a few hours after the onset of starvation and are defined by their requirement for Ca 2+ or Mg 2+ , respectively [4,5]. While CadA is the first to appear and is responsible for the Ca 2+ -mediated cell-cell adhesion system, the adhesion molecule responsible for the slightly later appearing Mg 2+ -mediated cell-cell adhesion system, remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Loss of FrmA leads to increased cell-cell adhesion and impaired multi-cellular development of Dictyostelium cells

Patel

Brunton

2008

Cell. Mol. Life Sci.

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Cell-cell adhesion is a critical property of all multi-cellular organisms and its correct regulation is critical during development, differentiation, tissue building and maintenance, and many immune responses. The multi-talin-like FERM domain containing protein, FrmA, is required during starvation-induced multi-cellular development of Dictyostelium cells. Loss of FrmA leads to increased cell-cell adhesion and results in impaired multi-cellular development, slug migration and fruiting bodies. Further, mixing experiments show that FrmA null cells are excluded from the apex of wild-type mounds, to which cells that normally form the organising centre known as the tip sort. These data suggest a critical role for FrmA in regulating cell-cell adhesion, multi-cellular development and, in particular, the formation of the organising centre known as the tip.

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“…The adhesion receptors are typically connected to the cytoskeleton via adapter proteins, such as the catenins that link cadherin complexes to the actin cytoskeleton (11). In Dictyostelium, cell-cell contacts are first formed by the Ca 2ϩ -dependent adhesion molecule DdCAD-1 (12)(13)(14) and distinct Mg 2ϩ -dependent adhesion sites (15). The Ca 2ϩ /Mg 2ϩ -independent adhesion molecule gp80 is expressed at the onset of the chemotactic migration stage (16) and mediates strong cell-cell adhesion (17,18) via trans-homophilic interaction (19,20).…”

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confidence: 99%

Cytoskeleton Interactions Involved in the Assembly and Function of Glycoprotein-80 Adhesion Complexes in Dictyostelium

Harris¹,

Ravandi²,

Awrey³

et al. 2003

Journal of Biological Chemistry

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Adhesion complexes typically assemble from clustered receptors that link to the cytoskeleton via cytoplasmic adapter proteins. However, it is unclear how phospholipid-anchored adhesion molecules, such as the Dictyostelium receptor gp80, interact with the cytoskeleton. gp80 has been found to form adhesion complexes from raftlike membrane domains, which can be isolated as a Triton X-100-insoluble floating fraction (TIFF). We report here that the actin-binding protein ponticulin mediates TIFF-cytoskeleton interactions. Analysis of gp80-null cells revealed that these interactions were minimal in the absence of gp80. During development, gp80 was required to enhance these interactions as its adhesion complexes assembled. Whereas ponticulin and gp80 could partition independently into TIFF, gp80 was shown to recruit ponticulin to cell-cell contacts and to increase its partitioning into TIFF. However, these proteins did not co-immunoprecipitate. Furthermore, sterol sequestration abrogated the association of ponticulin with TIFF without affecting gp80, suggesting that sterols may mediate the interactions between ponticulin and gp80. In ponticulin-null cells, large gp80 adhesion complexes assembled in the absence of ponticulin despite the lack of cytoskeleton association. We propose that such nascent gp80 adhesion complexes produce expanded raftlike domains that recruit ponticulin and thereby establish stable cytoskeleton interactions to complete the assembly process.

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Two distinct adhesion systems are responsible for EDTA-sensitive adhesion in Dictyostelium discoideum

Cited by 21 publications

References 38 publications

Sociogenomics of self vs. non-self cooperation during development of Dictyostelium discoideum

Sociogenomics of self vs. non-self cooperation during development of Dictyostelium discoideum

Loss of FrmA leads to increased cell-cell adhesion and impaired multi-cellular development of Dictyostelium cells

Cytoskeleton Interactions Involved in the Assembly and Function of Glycoprotein-80 Adhesion Complexes in Dictyostelium

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