Two-dimensional gel studies of genetic variation in the plasma proteins of Amerindians and Japanese

Asakawa, Jun‐ichi; Takahashi, Norio; Rosenblum, Barnett B.; Neel, James V.

doi:10.1007/bf00273446

Cited by 64 publications

(38 citation statements)

References 25 publications

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“…The frequencies of the three common alleles, s2, ~3 and s4, are similar to those observed in Hiroshima (Asakawa et al, 1985). However, it is noteworthy that three out of 187 subjects (1.5~) were found to have apo E5 or apo E7 in heterozygous state.…”

Section: Resultssupporting

confidence: 76%

“…Three common alleles, designated s2, ~3 and ~4, have been demonstrated to exist in various populations Wardell et al, 1982;Ghiselli et al, 1982;Cumming and Robertson, 1984;Asakawa et al, 1985;Sing and Davignon, 1985). The three common alleles, ~2, e3 and e4, code for three common apo E isoforms, apo E2, apo E3 and apo E4, respectively and determine six apo E phenotypes (Zannis et al, 1981 ;Zannis et al, 1982;Utermann et al, 1982).…”

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confidence: 99%

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Frequencies of apolipoproteins E5 and E7 in apparently healthy Japanese

et al. 1985

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SummaryHuman apolipoprotein (apo) E plays an important role in the metabolism of cholesterol and other lipids. Apo E5 and apo E7 are genetic variants of apo E and have been detected in about 5~ of Japanese patients with hyperlipidemia and ischemic heart disease. The existence of apo E5 and apo E7, however, had not been reported in apparently healthy individuals except for a few family members of the patients with apo E5 or apo E7. It has been suggested that apo E5 and apo E7 are closely related to the development of atherosclerosis. The purpose of this study is to investigate the frequency of apo E5 and apo E7 in apparently healthy Japanese and to analyze serum lipid levels of the individuals with apo E5 or apo E7.The apo E phenotypes of 197 apparently healthy Japanese adults were determined by two-dimensional gel electrophoresis. The gene frequencies ofapo E were: ~3, 0.843; ~4, 0.112; ~2, 0.038; e5, 0.006; ~7, 0.0035. Three out of 187 subjects (1.5~) were found to have apo E5 or apo E7 in heterozygous state. Two of them were heterozygous with apo E3 (apo E3/5 and apo E3/7) and the both had normal serum lipid levels, though they were more than 50 years old. The other individual was heterozygous with apo E2 (apo E2/5) and had mild hypertriglyceridemia. As to myocardial infarction, angina pectoris and cerebral infarction, no clinically abnormal findings were detected in all the three individuals with apo E5 or apo E7.The data suggest that the frequency of the individuals with apo E5 or apo E7 is of the order of 1 X and much higher than that of the homozygotes with apo E2/2 in Japanese. The data also indicate that further genetic, epidemiologic and clinical studies are required to determine whether ~5 and ~7 act as a dominant major gene, as a recessive major gene, or as

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Section: Resultssupporting

confidence: 76%

mentioning

confidence: 99%

Frequencies of apolipoproteins E5 and E7 in apparently healthy Japanese

et al. 1985

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“…In Japanese, the s3 allele is the most common and the s4 allele is the second commonest, as in other populations studied thus far (Asakawa et al, 1985;Tsuchiya et aI., 1985). In Caucasian populations, there is growing evidence that the s4 allele might be involved in predisposing one to hyperlipidemia (Ghiselli et al, 1982a(Ghiselli et al, , 1982bUtermann et al, 1984;Sing and Davignon, 1985).…”

Section: Introductionmentioning

confidence: 55%

“…Three common alleles, designated ~2, s3, and ~4, code for three common apo E isoforms, apo E2, apo E3 and apo E4, respectively, and determine six apo E phenotypes (Zannis et al, 1982). The three common alleles have been demonstrated to exist in various populations Wardell et al, 1982;Ghiselli et al, 1982a;Cumming and Robertson, 1984;Sing and Davignon, 1985;Asakawa et al, 1985;Tsuchiya et aI., 1985).…”

Section: Introductionmentioning

confidence: 99%

Apolipoprotein e phenotypes and serum lipid levels in apparently healthy male adults in Tokyo

et al. 1986

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SummaryThis study was performed to investigate whether the ~4 allele of the apolipoprotein (apo) E predisposes Japanese male adults in large cities to hyperlipidemia. The apo E phenotypes and serum total lipid levels were determined using blood samples obtained after an overnight fast from 85 apparently healthy Japanese male civil servants in Tokyo. The frequency of hyperlipidemia was 50.0~ in 20 subjects with the apo E4-positive phenotype and 25.8~ in 62 subjects with the apo E3/3 phenotype. The difference is statistically significant (p<0.05). The mean (_+SD) serum triglyceride levels, which were calculated on the subjects with serum triglyceride lower than 250 mg/dl, was 132.8_+5.1 mg/dl in 18 subjects with the apo E4-positive phenotype and 108.4_+43.0 mg/dl in 58 subjects with the apo E3/3 phenotype. The difference is also statistically significant (p<0.05). The data suggest that the e4 allele might predispose Japanese adults in large cities to hyperlipidemia.

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“…In the apo E gene, there are three common alleles, designated s2, s3 and s4, which code for apo E2, apo E3, and E4, respectively (Zannis et al, 1982). The apo E3 allele is the most common and the apo E4 allele is the second commonest in Japanese (Asakawa et al, 1985;Tsuchiya et al, 1985;Eto et al, 1986). The apo E4 allele is present in about 20~ of populations.…”

Section: Introductionmentioning

confidence: 99%

Association of the apolipoprotein E4 allele with hypercholesterolemia in apparently healthy male adults in Tokyo

et al. 1987

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SummaryThis study was performed to investigate whether the apolipoprotein (apo) E4 allele is associated with hypercholesterolemia in Japanese male adults in large cities. The apo E phenotypes and serum lipid levels were analyzed on 142 apparently healthy male civil servants working in Tokyo. The prevalence of hypercholesterolemia (serum total cholesterol level>250 mg/dl) were 4.7~ in 106 apo E4-absent subjects and 19.4~ in 36 apo E4-present subjects (p=0.012). The prevalence of hypercholesterolemia were 5.3~o in 94 subjects with the apo E3/3 phenotype and 20.0~ in 30 subjects with the apo E3/4 phenotype (p=0.023). In addition, mean serum total cholesterol levels were significantly higher in the apo E4-present subjects than in the apo E4-absent subjects (213_+29.1 versus 199_+36.0, p <0.05). The data suggest that the apo E4 allele is associated with hypercholesterolemia in Japanese male adults working in large cities.

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Two-dimensional gel studies of genetic variation in the plasma proteins of Amerindians and Japanese

Cited by 64 publications

References 25 publications

Frequencies of apolipoproteins E5 and E7 in apparently healthy Japanese

Frequencies of apolipoproteins E5 and E7 in apparently healthy Japanese

Apolipoprotein e phenotypes and serum lipid levels in apparently healthy male adults in Tokyo

Association of the apolipoprotein E4 allele with hypercholesterolemia in apparently healthy male adults in Tokyo

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