Two-dimensional gel electrophoresis of dog plasma proteins: Genetic polymorphism of an α1-protease inhibitor and another postalbumin

Abstract

Two‐dimensional electrophoretic analysis of dog plasma proteins was done by a first‐dimension separation in agarose gel (pH 5.4) followed by a second‐dimension separation in horizontal poly‐acrylamide gel (pH 9.0). Two groups of α1‐globulins, designated Pi‐1 and Pi‐2, were found to be protease inhibitors. Extensive genetic polymorphism of Pi‐1 and of another unidentified α‐globulin, tentatively designated postalbumin (Pa), was observed. Family data supported the hypothesis that Pi‐1 and Pa polymorphisms were controlled by codominant, autosomal alleles. Three Pi‐1 alleles and two Pa alleles were observed in 60 breeds (1119 dogs) studied. Each of the Pi‐1 alleles gave rise to a single strong fraction while each of the Pa alleles showed a major and a minor anodic fraction. The present study, along with earlier reports, showed that some of the proteins and enzymes (albumin, transferrin, eserine resistant esterase, α1‐protease inhibitor and postalbumin) in plasma exhibit considerably more polymorphism than that reported for hemoglobin and some of the red cell and tissue enzymes of the domestic dog.

Three new plasma protein polymorphisms in domestic foxes, detected by a simple method of 2D horizontal electrophoresis

“…It is apparent that the 2D patterns of silver and arctic fox plasma proteins are extremely similar. The 2D patterns of fox plasma proteins are also much similar to those previously reported for dog plasma proteins by the same method (JUNEJA et al 1981. The relative mobilities of ten different plasma proteins are shown.…”

“…1 and 2), but there was no evidence for a genetic polymorphism of this protein. The fox plasma Pi1 and Pi2 are apparently homologus, respectively, to dog plasma Pi1 and Pi2 proteins reported by JUNEJA et al (1981). Three other, as yet midentitied plasma proteins of foxes, were tentatively designated as postalbumin 1 (Pal), pretransferrin 1 (Prtl) and pretransferrin 2 (Prt2) ( Fig.…”

Polymorphism of a serum protease inhibitor system in Alaskan wolves

“…Twenty-five wolves had a type which resembled the heterozygous FI type of dogs (JUNEJA et al 1981). In three wolves, the most cathodal type of dogs, called S (JUNEJA et al 1981), was indicated. The remainder of the 123 wolves did not appear with Pi types which could be safely diagnozed.The 11 samples collected in 1986 gave, however, Fig.…”

“…Among the wolves, 34 had aphenotype similar to the F type in dogs. Twenty-five wolves had a type which resembled the heterozygous FI type of dogs (JUNEJA et al 1981). In three wolves, the most cathodal type of dogs, called S (JUNEJA et al 1981), was indicated.…”

“…Many of the samples, however, had too weak and indistinct zones. In the corresponding system of dogs, six phenotypes explained by three alleles have been reported (JUNEJA et al 1981). Among the wolves, 34 had aphenotype similar to the F type in dogs.…”