Two-Dimensional Gel Electrophoresis and Mass Spectrometry in Studies of Nanoparticle-Protein Interactions

Karlsson, Helén; Ljunggren, Stefan; Ahrén, Maria; Ghafouri, Bijar; Lindahl, Mattias; Ljungman, Anders

doi:10.5772/38085

Cited by 3 publications

(3 citation statements)

References 58 publications

(52 reference statements)

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“…Since metal exposure may result in detectable metals in blood and urine, it is highly important to study metal related health effects in the vascular system. Studies of plasma protein-nanoparticle interactions have shown that the HDL protein apo A-I is able to interact with metal particles in plasma [ 23 ]. Therefore, apo A-I analysis was included in this study ( Table 4 ).…”

Section: Discussionmentioning

confidence: 99%

“…Besides its protective effects against cardiovascular disease due to the reverse cholesterol transport, apo A-I situated on high-density lipoprotein (HDL) has important effects in immune defense [ 20 , 21 ]. Furthermore, apo A-I is present on most studied nanoparticles in plasma [ 22 , 23 ]. Therefore, apo A-I may be involved in nanoparticles clearance via the scavenger receptor B-I on hepatocytes, followed by distribution via bile to feces similar to excess cholesterol and endotoxins, making it interesting for metal occupational exposure.…”

Section: Introductionmentioning

confidence: 99%

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Metal additive manufacturing and possible clinical markers for the monitoring of exposure-related health effects

et al. 2021

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Additive manufacturing (AM) includes a series of techniques used to create products, in several different materials, such as metal, polymer or ceramics, with digital models. The main advantage of AM is that it allows the creation of complex structures, but AM promises several additional advantages including the possibility to manufacture on demand or replacing smaller worn parts by directly building on an existing piece. Therefore, the interest for and establishment of AM is rapidly expanding, which is positive, however it is important to be aware that new techniques may also result in new challenges regarding health and safety issues. Metals in blood and possible clinical effects due to metal exposure were investigated in AM operators at one of the first serial producing AM facilities in the world during two consecutive years with implementation of preventive measures in-between. As comparison, welders and office workers as control group were investigated. Health investigations comprised of surveys, lung function tests, antioxidant activity and vascular inflammation as well as renal- and hepatic function analysis. AM operators had significantly reduced nickel levels in blood (10.8 vs 6.2 nmol/L) as well as improved lung function (80 vs 92% of predicted) from year 1 to year 2. This is in line with previously published results displaying reduced exposure. Blood cobalt and nickel levels correlated with previously reported urinary levels, while blood chromium did not. Multivariate modelling showed that blood cobalt, antioxidant/inflammatory marker serum amyloid A1/serum paraoxonase/arylesterase 1 activity and the hepatic markers aspartate transaminase, alanine transaminase, and alkaline phosphatase were higher in AM operators compared to controls. The study show that the selected clinical analyses could function as a complement to metal analyses in biological fluids when investigating exposure-related health effects in AM operators. However, validation in larger cohorts is necessary before more definite conclusions could be drawn.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metal additive manufacturing and possible clinical markers for the monitoring of exposure-related health effects

et al. 2021

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“…Thus the biodistribution of nanoparticles is significantly influenced by their interaction with plasma proteins, notably HDL proteins apoA-I and apoE. These, amongst others, have been found to recognise and interact with carbon nanotubes, a number of metal oxides (Karlsson et al 2012) and PLGA (poly(lactic-co-glycolic acid)) nanoparticles (Sempf et al 2013) in in vitro plasma models. There are, as yet, no detailed studies confirming HDL neutralisation and clearance of engineered nanoparticles.…”

Section: Metal Oxides Carbon Nanotubes and Plga Nanoparticlesmentioning

confidence: 99%

Functionality of HDL: Antioxidation and Detoxifying Effects

Karlsson

Kontush

James

2014

Handbook of Experimental Pharmacology

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Genotoxicity and gene expression analyses of liver and lung tissues of mice treated with titanium dioxide nanoparticles

Yan

Ding

et al. 2016

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Titanium dioxide nanoparticles (TiO NPs) are used in paints, plastics, papers, inks, foods, toothpaste, pharmaceuticals and cosmetics. However, TiO NPs cause inflammation, pulmonary damage, fibrosis and lung tumours in animals and are possibly carcinogenic to humans. Although there are a large number of studies on the toxicities of TiO NPs, the data are inconclusive and the mechanisms underlying the toxicity are not clear. In this study, we used the Comet assay to evaluate genotoxicity and whole-genome microarray technology to analyse gene expression pattern in vivo to explore the possible mechanisms for toxicity and genotoxicity of TiO NPs. Mice were treated with three daily i.p. injections of 50 mg/kg 10 nm anatase TiO NPs and sacrificed 4 h after the last treatment. The livers and lungs were then isolated for the Comet assay and whole genome microarray analysis of gene expression. The NPs were heavily accumulated in liver and lung tissues. However, the treatment was positive for DNA strand breaks only in liver measured with the standard Comet assay, but positive for oxidative DNA adducts in both liver and lung as determined with the enzyme-modified Comet assay. The genotoxicity results suggest that DNA damage mainly resulted from oxidised nucleotides. Gene expression profiles and functional analyses revealed that exposure to TiO NPs triggered distinct gene expression patterns in both liver and lung tissues. The gene expression results suggest that TiO NPs impair DNA and cells by interrupting metabolic homeostasis in liver and by inducing oxidative stress, inflammatory responses and apoptosis in lung. These findings have broad implications when evaluating the safety of TiO NPs used in numerous consumer products.

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Two-Dimensional Gel Electrophoresis and Mass Spectrometry in Studies of Nanoparticle-Protein Interactions

Cited by 3 publications

References 58 publications

Metal additive manufacturing and possible clinical markers for the monitoring of exposure-related health effects

Metal additive manufacturing and possible clinical markers for the monitoring of exposure-related health effects

Functionality of HDL: Antioxidation and Detoxifying Effects

Genotoxicity and gene expression analyses of liver and lung tissues of mice treated with titanium dioxide nanoparticles

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