Two-dimensional electrophoretic protein patterns of reciprocal hybrids of the mouse strains DBA and C57BL

Vogel, Tilman; Klose, Joachim

doi:10.1007/bf02399813

Cited by 11 publications

(5 citation statements)

References 43 publications

(34 reference statements)

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“…The use of offspring from reciprocal crosses between inbred strains of mice provides a methodology to examine parent of origin effects that relies on underlying gene polymorphisms and not upon a genetic manipulation. Reciprocal cross strategies have been employed to demonstrate parental effects on a number of phenotypes at the behavioural (McGill & Manning 1976;Banko et al 1997), cellular (Bander et al 1989;McDonald & Jackson 1994), and molecular level (Vogel & Klose 1992). In addition, we also observed POE differences in weight between our reciprocal cross F 1 mice (A. R. Isles, M. J. Baum, D. Ma, A. Szeto, E. B.…”

Section: Discussionsupporting

confidence: 66%

A possible role for imprinted genes in inbreeding avoidance and dispersal from the natal area in mice

Isles

Baum

et al. 2002

Proc. R. Soc. Lond. B

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The expression of a subset of mammalian genes is subject to parent of origin effects (POE), most of which can be explained by genomic imprinting. Analysis of mutant animals has demonstrated that a number of imprinted genes influence brain development and behaviour. Here we provide evidence for POE on olfactory related behaviour and sensitivity to maternal odour cues. This was investigated by examining the odour preference behaviour of reciprocal cross F(1) mice made by embryo transfer to genetically unrelated foster parents. We determined that both adult males and females show an avoidance of female urinary odours of their genetic maternal but not paternal origin. This was found not to be due to any previous exposure to these odours or due to self-learning, but may be related to direct effects on the olfactory system, as reciprocal F(1) males show differential sensitivity to female odour cues. Currently the most robust theory to explain the evolution of imprinting is the conflict hypothesis that focuses on maternal resource allocation to the developing foetus. Kinship considerations are also likely to be important in the selection of imprinted genes and we discuss our findings within this context, suggesting that imprinted genes act directly on the olfactory system to promote post-weaning dispersal from the natal area.

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Section: Discussionsupporting

confidence: 66%

A possible role for imprinted genes in inbreeding avoidance and dispersal from the natal area in mice

Isles

Baum

et al. 2002

Proc. R. Soc. Lond. B

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“…The present study is part of a long-term endeavor aimed at the identification of murine strain-specific protein variants, which reveal a mode of inheritance compatible with the concept of genomic imprinting, i.e., a maternal or paternal transmission [33,34]. Silver-stained 2-DE patterns of cytosolic liver proteins were investigated in 8 parental mice (2 mice of each gender) of the 2 subspecies M. musculus musculus (inbred strain: Calcinato B) and M. musculus domesticus (inbred strain: Aarhus) and in their offspring (25 F 1 hybrids: 14 females, 11 males).…”

Section: Resultsmentioning

confidence: 99%

Selenium‐binding protein 2, the major hepatic target for acetaminophen, shows sex differences in protein abundance

Mattow¹,

Demuth²,

Haeselbarth³

et al. 2006

Electrophoresis

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Liver samples from female and male mice of two subspecies, Mus musculus musculus and Mus musculus domesticus, were investigated by a combination of 2-DE and MALDI-MS. The image analysis of the generated 2-DE patterns revealed several protein spots with significant differences in intensity/abundance between the sexes. Seven protein spots, which were prominent in 2-DE patterns of male mice, but which showed very low intensities in females, were identified as selenium-binding protein 2 (SBP2) also known as 56-kDa acetaminophen-binding protein. Edman degradation indicated that at least three of these protein spots represent N-terminally truncated SBP2 variants. Furthermore, it was shown that the observed differences in SBP2 abundance correlate with sex differences in transcription of the gene encoding SBP2, selenbp2, as revealed by RT-PCR and restriction digest as well as sequence analysis of the products. Since SBP2 has been described as the major target for acetaminophen in mouse liver cytosol, these findings are discussed with respect to their possible relevance for sex differences in acetaminophen-mediated toxicity, which have been described in a variety of mammals including mice and rats.

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“…The 2-DE patterns of adult liver proteins and proteins of embryonic and extraembryonic tissues of day-10 embryos have been investigated in reciprocal hybrids of the two inbred mouse strains D2 and B6 in order to examine whether strain-specific protein variants occur which exhibit modes of inheritance compatible with the concept of genomic imprinting [5]. Three proteins of this type, forming particular groups of spots in 2-DE patterns, were found and analyzed in more detail (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…by collecting more material (amnions) and by increasing the sensitivity of the detection system (silver staining) for small restriction fragments. As a method for detecting parent-specific inheritance in mice, 2-DE of proteins was also employed by Vogel and Klose [5] and Romer 1361. The same strains of the mouse (D2 and B6) as used in the present study were investigated, including reciprocal hybrids.…”

Section: Discussionmentioning

confidence: 99%

A novel strategy to identify maternal and paternal inheritance in the mouse

et al. 1995

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A novel strategy for identifying proteins which reveal maternal or paternal inheritance in the mouse is presented. Using two-dimensional electrophoresis we investigated protein expression patterns of adult liver and different embryonic and extraembryonic tissue in C57BL/6Crl and in DBA/2Crl mice, as well as in their reciprocal hybrids. We found three groups of protein spots which showed maternal or paternal inheritance of quantitative variations. These proteins were characterized by N-terminal or internal amino acid sequencing, by determination of the amino acid composition, by glycoprotein staining and RNA expression analysis. The three proteins identified were: alpha-enolase, cyclophilin and beta-group hemoglobins. The parental effects observed for alpha-enolase and cyclophilin were found to be due to parent-specific post-translational modifications of these proteins. For the beta-group hemoglobins our results suggested parental effects on the transcriptional level.

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Two-dimensional electrophoretic protein patterns of reciprocal hybrids of the mouse strains DBA and C57BL

Cited by 11 publications

References 43 publications

A possible role for imprinted genes in inbreeding avoidance and dispersal from the natal area in mice

A possible role for imprinted genes in inbreeding avoidance and dispersal from the natal area in mice

Selenium‐binding protein 2, the major hepatic target for acetaminophen, shows sex differences in protein abundance

A novel strategy to identify maternal and paternal inheritance in the mouse

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